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InvisibleCDS
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Registered: 09/05/08
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Accidentally mixed strains of grain in bulk sub, what will happen???
    #9429456 - 12/14/08 10:11 AM (15 years, 3 months ago)

So, hypothetically speaking, if one were to have some straw/coir/poo sub that he was inoculating with grain spawn (rye), and instead of having 6 layers of grain and 7 layers of sub of all the same strain, he accidentally added in one quart of a different strain, what, if any, adverse effects will happen?

The layers are arranged like so:

Sub Layer Top
Strain A
Sub Layer
Strain A
Sub Layer
***Strain B***
Sub Layer
Strain A
Sub Layer
Strain A
Sub Layer
Strain A
Sub Layer Bottom


I'm not sure where the strain B layer is in regards to the other layers, but I'm pretty sure it's not on top or on bottom...

Will this have detrimental effects on the rate of colonization and/or the yield?

Will the strain with the most mass (strain A) take over?; and therefore will all the fruits be strain A, or will they be a proportional mix of the two?



-Thanks

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Offlinegrod31
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Re: Accidentally mixed strains of grain in bulk sub, what will happen??? [Re: CDS]
    #9429464 - 12/14/08 10:14 AM (15 years, 3 months ago)

no way for sure to say what will happen. you will still get mushrooms though


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread

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Offlinetrich_cultivator
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Registered: 09/08/08
Posts: 469
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Re: Accidentally mixed strains of grain in bulk sub, what will happen??? [Re: grod31]
    #9429473 - 12/14/08 10:18 AM (15 years, 3 months ago)

yes you will get the two different mushrooms, since it seems like strain A is in most of the substrate, so you will get mostly A but there will be strain B in there as well. One of my friends did this with some jars I gave him, and he got all 3 different types in the same tub.


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Offlinedrjugglz
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Re: Accidentally mixed strains of grain in bulk sub, what will happen??? [Re: grod31]
    #9429475 - 12/14/08 10:18 AM (15 years, 3 months ago)

the strains will compete for nutrients and a dueling match till the death!


--------------------
“To use your head you have to go out of your mind.”
~ Timothy Leary

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OfflineRogerRabbitM
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Re: Accidentally mixed strains of grain in bulk sub, what will happen??? [Re: drjugglz]
    #9429506 - 12/14/08 10:24 AM (15 years, 3 months ago)

That's what happens every time you use multi-spore inoculation anyway.  A strain isn't defined by the name some moron wrote on the print.  A strain is a pairing of compatible hyphae.  There's at least two active threads right now showing that using multiple 'strains' will have no effect, either negative or positive.  Cubes are cubes. So-called strains of the same species don't compete, fight, or otherwise fuck with each other.  You'll never notice any difference from your 'mistake'.
RR

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