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OfflineHallucinogenist
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Syringe tips...
    #9418563 - 12/12/08 01:43 PM (15 years, 3 months ago)

Okay, I've read the teks and all, and I just received an OI print.  Any PERSONAL pointers you all'd recommend when I make my syringe?

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OfflineDamion5050
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Re: Syringe tips... [Re: Hallucinogenist]
    #9418576 - 12/12/08 01:45 PM (15 years, 3 months ago)


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Offlinekrypto2000
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Re: Syringe tips... [Re: Damion5050]
    #9418827 - 12/12/08 02:17 PM (15 years, 3 months ago)

Here's what I do:

Put the print in a ziplock bag (it should be sterile enough out of the box ime) and put ur print inside.

Boil some water and suck it into your syringe and empty it out a few times to make sure the syringe is sterile, then let the final pull cool to room temp. Once cool squirt the sterile water into the ziplock and seal it shut.

Now rub the print inside the bag to get it mixed up well into the water. Choose a corner of the bag and wipe it down with some alcohol and do the same for the needle. It's a good idea to flame the tip of the needle too just in case.

Now shove that fucker through the corner and suck in your water. Congratulations, you have a spore syringe.

This method has always worked for me and I've yet to get a contaminated syringe. I also just make my spore print in open air and don't use a glove box for any of this. I've only done about 3-5 syringes though, but so far so good. :thumbup:

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OfflineDamion5050
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Re: Syringe tips... [Re: krypto2000]
    #9418850 - 12/12/08 02:20 PM (15 years, 3 months ago)

Quote:

krypto2000 said:
Here's what I do:

Put the print in a ziplock bag (it should be sterile enough out of the box ime) and put ur print inside.

Boil some water and suck it into your syringe and empty it out a few times to make sure the syringe is sterile, then let the final pull cool to room temp. Once cool squirt the sterile water into the ziplock and seal it shut.

Now rub the print inside the bag to get it mixed up well into the water. Choose a corner of the bag and wipe it down with some alcohol and do the same for the needle. It's a good idea to flame the tip of the needle too just in case.

Now shove that fucker through the corner and suck in your water. Congratulations, you have a spore syringe.

This method has always worked for me and I've yet to get a contaminated syringe. I also just make my spore print in open air and don't use a glove box for any of this. I've only done about 3-5 syringes though, but so far so good. :thumbup:




Nice and Easy.. :thumbup:

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InvisibleShroominit
Part Time Mycologist


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Registered: 11/29/08
Posts: 4,662
Re: Syringe tips... [Re: Damion5050]
    #9419283 - 12/12/08 03:40 PM (15 years, 3 months ago)

My PERSONAL suggestion is to be clean about it,  as krypto said, you don't have to be completely lab-sterile with it, just make sure you don't do anything stupid.

That being said, I use the method:
Sterilize a jar of water, syringes and shotglass. (in foil)
after they cool, move to glove box with your print.
take foil off, scrape some spores into the glass, I use a piece of wire in a loop (DIY inoculating loop) and then suck water from the jar, put it in the shotglass, then back into the syringe with it.

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