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InvisibleOmnicracker
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LONG TERM STORAGE: LC->Vacutainer + sterile water
    #9401888 - 12/09/08 07:06 PM (15 years, 3 months ago)

ive been exploring my options for these vacutainers i have, for long term storage of my master cultures.

those familiar should know Agar's tek for replacing the leftover LC in the vacutainer with sterile water.

http://www.shroomery.org/forums/showflat.php/Number/4213129#4213129

My addition here is more of a subtraction actually.  Why even inject the vacutainer and wait for it to settle out?

First, Sterilize a syringe full of water or aspirate out of a PCed jar.  Stick it in the vacutainer and it will automatically pull in a few ccs of H2O. 

Then aspirate from your LC( with a new/sterile syringe), recap the syringe and let it sit upright (needle pointing down) for a day.  All the myc will will settle down over the needle and will be the first thing to come out.  just a drop or two.  no need to build any air-port  or aspirate OUT of a vacutainer.  until its time to use of course.

thought id share that.  seems like a good idea to me.


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InvisibleFooMan
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: Omnicracker]
    #9402002 - 12/09/08 07:25 PM (15 years, 3 months ago)

Simple. I like it :thumbup:


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InvisibleOmnicracker
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: FooMan]
    #9402064 - 12/09/08 07:40 PM (15 years, 3 months ago)

yeah, so simple im amazed Agar didnt think of it.  he is the king of simplified DIY.


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OfflineRogerRabbitM
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: Omnicracker]
    #9402101 - 12/09/08 07:48 PM (15 years, 3 months ago)

Give your idea a try.  It will be interesting in a few years to see how the cultures do without any air.  I have some cultures on distilled water now, but in mason jars with filtered gas exchange.  They're approaching three years old and still viable.
RR


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InvisibleOmnicracker
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: RogerRabbit]
    #9403204 - 12/09/08 10:22 PM (15 years, 3 months ago)

i could buy some of these later on for air exchange
<img>http://www.shroomery.org/forums/thumbs/07-004/946355084-thumb_air_ports.jpg</img>

add them in a few months and it shouldnt be a problem.  as for now, i just need to get some things in a more permanent storage.  isolation is too much work/space do have to redo every time i move around. 

FooMan, im watching the 'addendum' right now...
when i seen the first one i crapped my pants.


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InvisibleOmnicracker
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: Omnicracker]
    #9405026 - 12/10/08 10:01 AM (15 years, 3 months ago)

thats fucked up... everytime i try to watch the movie, my mac crashes halfway through.  seriously, ive tried 4 times and then the cpu freezes and the screen goes to CMYK test pattern.  im freaking out, someone doesnt want that video to be seen.


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InvisibleFooMan
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: Omnicracker]
    #9411583 - 12/11/08 09:09 AM (15 years, 3 months ago)

They're watching :tinfoil:


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Offlinesolumvita
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Re: LONG TERM STORAGE: LC->Vacutainer + sterile water [Re: FooMan]
    #9411693 - 12/11/08 09:39 AM (15 years, 3 months ago)

Great idea, and simple too!


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