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Offlinetrippatient
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anybody grow invitro before?
    #9366314 - 12/04/08 03:14 PM (15 years, 2 months ago)

im considering growing invitro for the first time. this would be my third grow. my first two contamed and im not too eager to grow again only to get fucked by trich again.
so i read about invitro growing and its least likely to contam growing invitro due to lack of exposure to bad air or whatever... so that sparks my interest more than anything.

so my question is does anybody have any experience growing invitro? what are the pros and cons of growing like this?

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Invisiblearp180
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Registered: 10/17/08
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Re: anybody grow invitro before? [Re: trippatient]
    #9366382 - 12/04/08 03:25 PM (15 years, 2 months ago)

If you messed up the pf tek due to contams, the problem isn't which type of grow, it's your sterile procedure.  Once anything is fully colonized, it will be very resistant to contamination. What step where you getting contams at???


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


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OfflineCascadian
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Re: anybody grow invitro before? [Re: trippatient]
    #9366385 - 12/04/08 03:26 PM (15 years, 2 months ago)

100% colonized, new mycelium is very resistant to contamination.  I think you're more likely to see trich either during colonization, or after you've had a couple of flushes.

if your grows are getting triched before they ever flush, growing invitro isn't going to help you then.  Your problem is either in your substrate (not enough sterilization), your containers (improper/wet filters), or what you are using to inoculate with (spores/LC not sterile).

But I might be wrong.

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InvisibleJ3illy
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Registered: 10/18/08
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Re: anybody grow invitro before? [Re: trippatient]
    #9366388 - 12/04/08 03:27 PM (15 years, 2 months ago)

Well I've never* done it, but read about quite a few cases.  There's a few things that are considered invitro.  For example, you can take the tops right off the jars like this.



You can grow inside a jar that has a decent amount of room in it like this.



But if you're thinking about doing it in a PF-tek jar, it's not a good idea.  There's barely any room inside them, so there's no FAE.  It usually happens by accident if someone forget about their jars for a few days or something. 

It's not really recommend cuz the yields are very low, and who knows if and when they'll produce cuz you're cutting off a major pinning trigger.  If you really wanna do it,  I'd suggest like the 1st pic where you take the top of the lid off.

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Offlinegofudgeyourself
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Re: anybody grow invitro before? [Re: trippatient]
    #9366469 - 12/04/08 03:40 PM (15 years, 2 months ago)

YEAW BOY!!!

lol i do in vitro sometimes, heres some pics





invitro harvest, 26.5g wet.

they obviously arent as meaty and thick but its probably the laziest and easiest way to grow mushrooms, it also allows for alot of fun innovation (jar placement, riser construction, humidity and temp experiments).....alot of people knock it but if you just want shrooms without alot of work then its the best way.

as for contams, it would be more resistant imo...theres nothing landing on your cakes, i would think that if you made them in a clean place you would have a higher success rate. good luck


are we allowed to post links to thenook? my apologies if not but this is a VERY helpful and old school place for in vitro stuff.
http://www.thenook.org/archives/2386.html

Edited by gofudgeyourself (12/04/08 03:50 PM)

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Invisiblearp180
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Re: anybody grow invitro before? [Re: J3illy]
    #9366503 - 12/04/08 03:45 PM (15 years, 2 months ago)

jilly, glad to see you took the suggestion.  Best of luck with your next grow


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


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Offlinegrod31
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Registered: 12/06/07
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Re: anybody grow invitro before? [Re: arp180]
    #9367088 - 12/04/08 05:11 PM (15 years, 2 months ago)



--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread

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Offlinetrippatient
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Re: anybody grow invitro before? [Re: grod31]
    #9368723 - 12/04/08 09:04 PM (15 years, 2 months ago)

i ALWAYS contam when i put the fully colonized cake in the FC. it never happens immediately, only after sitting for a week or two. theres nothing wrong with the sterilization procedure if it contams after a while like that right?
should i give casing a try? how do the chances of failure compare to PF tek jars?

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InvisibleJ3illy
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Registered: 10/18/08
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Re: anybody grow invitro before? [Re: trippatient]
    #9368762 - 12/04/08 09:10 PM (15 years, 2 months ago)

If you have fully colonized cakes contam'ing that quick you gotta figure out what's up.  Casing layers will contam too, and I'd guess are more likely to contam than a fully colonized cake, which should be pretty contam-resistant.

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OfflineDamion5050
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Re: anybody grow invitro before? [Re: J3illy]
    #9368769 - 12/04/08 09:11 PM (15 years, 2 months ago)

If a cake that is fully colonized gets contaminated than my money is on the fact it is not fully colonized on the inside..

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Offlinetrippatient
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Re: anybody grow invitro before? [Re: trippatient]
    #9368828 - 12/04/08 09:19 PM (15 years, 2 months ago)

thanks for the links to the nook grod and fudge, really useful. i think im gonna do an invitro grow. why not? the pics on the nook show some pretty mighty shrooms so ehhh im doin it:)

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InvisibleJ3illy
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Registered: 10/18/08
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Re: anybody grow invitro before? [Re: trippatient]
    #9368890 - 12/04/08 09:28 PM (15 years, 2 months ago)

If you wanna do invitro why not just do Mycobags?  There'll be no contam risk and your yields would prob. be higher

www.themadseason.com has some really good premade bags. 
$12/ea for 1, $9/ea for 5, and $8/ea for 10

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