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North Spore Shop: Bulk Substrate, Injection Grain Bag

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OfflineShrmanator
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are they okay?
    #934623 - 10/05/02 03:42 PM (18 years, 11 months ago)

id like to know if it would be okay to re-use jars that had contaminated substrate in them befor. like if i can just clean them out and sterlize them. any insight on this would be appciated


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OfflineAlbino_Jesus
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Re: are they okay? [Re: Shrmanator]
    #934644 - 10/05/02 03:55 PM (18 years, 11 months ago)

if they're sterilized, which means all the contams are killed, then of course.
if they're good glass, rather than boiling them, if you don't have an autoclave or anything, sticking them in the oven at 300+ for a while should work better because you're getting even up above boiling temp and making sure those jars are squeaky clean


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OfflineSeussA
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Re: are they okay? [Re: Albino_Jesus]
    #934881 - 10/05/02 06:03 PM (18 years, 11 months ago)

sticking them in the oven at 300+ for a while should work better because you're getting even up above boiling temp

This is a common misconception... dry heat sterilization (such as in an oven) works, but requires much longer times than steam sterilization. Steam transfers heat much better than dry air. If you use the oven, be sure to increase your sterilization time by a factor of two or three to compensate.


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Offlinebaraka
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Re: are they okay? [Re: Seuss]
    #935331 - 10/05/02 10:45 PM (18 years, 11 months ago)

my friend had bacteria in jars and just dumped the stuff out, rinsed and when thru dish washer.

They colonized fine.


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OfflineLSAuser
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Re: are they okay? [Re: Shrmanator]
    #935864 - 10/06/02 02:40 AM (18 years, 11 months ago)

I would throw em away without opening em as not to spread contams in the air. if you really want to reuse jars, open them outside far away from your house and wear a gas mask first then open the jars inside a bucket of bleach and let em sit in the bleach for a few hours


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North Spore Shop: Bulk Substrate, Injection Grain Bag


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