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Offlinejahfeelirie
meatwad

Registered: 10/05/02
Posts: 531
Last seen: 15 years, 10 months
longest colonization period, EVER.
    #934350 - 10/05/02 01:16 PM (18 years, 9 months ago)

Hey everyone,

I have 5 jars of PF and Indian strains colonizing for the past 5-6 weeks now. They havent even gotten halfway done. There are HUGE white areas in the jars, but they have either slowed to unnoticable growth, or they have stopped all together. I read thru the faq about temps and why they might be slowed/stopped. but im not sure that i have foudn the answer. Is 74/75 too low a temp for them? I used a very smalll amt when innoculating.... like 1 cc per jar hole. For the longest time they didnt show any growth, then they started colonizing, and well.. now they arent.

Maybe I should crumble/case? If so, might someone explain the tek to me? or point me in the right direction?

thx


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Offlinemyco_holic
mycologicalexplorer
Registered: 08/20/02
Posts: 138
Loc: SoCal
Last seen: 18 years, 4 months
Re: longest colonization period, EVER. [Re: jahfeelirie]
    #934362 - 10/05/02 01:24 PM (18 years, 9 months ago)

well....have you tried raising the temp a tad? maybe to the low 80's....also have you tried flipping your cakes?


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I believe in making the world safe for our children, but not our children's children, because I don't think children should be having sex.

Jack Handey.


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Offlinejahfeelirie
meatwad

Registered: 10/05/02
Posts: 531
Last seen: 15 years, 10 months
Re: longest colonization period, EVER. [Re: myco_holic]
    #934364 - 10/05/02 01:26 PM (18 years, 9 months ago)

Actually, I just moved one of the cakes to the top of my computer, and flipped it upside down. I will leave it for a few days and monitor its growth.

thx... any other ideas?


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OfflineTravelin man
Wonderer
Registered: 09/30/02
Posts: 10
Loc: Earth
Last seen: 18 years, 8 months
Re: longest colonization period, EVER. [Re: jahfeelirie]
    #934590 - 10/05/02 03:24 PM (18 years, 9 months ago)

Make sure moiture content is good, and allow for air exchange. if the mycelium can't get air, it cant grow


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Offlinejahfeelirie
meatwad

Registered: 10/05/02
Posts: 531
Last seen: 15 years, 10 months
Re: longest colonization period, EVER. [Re: Travelin man]
    #934768 - 10/05/02 04:54 PM (18 years, 9 months ago)

I do have masking tape over my jar holes... You think that might have caused a depletion of oxygen and slowed mycellium growth?

I will make sure to remove the tape when i get home and flip the cakes over.

any other suggestions?



thx
jfi


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OfflinedIZZY_ALpH
Living Dead

Registered: 09/23/02
Posts: 33
Last seen: 18 years, 7 months
Re: longest colonization period, EVER. [Re: jahfeelirie]
    #936295 - 10/06/02 12:55 PM (18 years, 9 months ago)

Yes, masking tape with inhibit the growth. If there is masking tape on the holes then the jars cant get rid of enough Co2. Also, I noticed wth one of my jars once that too much moisture will stop growth for sure.
~ALpH~


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"Well that's what he gets for not hailing to the chimp" - Homer Simpson-


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Offlinejahfeelirie
meatwad

Registered: 10/05/02
Posts: 531
Last seen: 15 years, 10 months
Re: longest colonization period, EVER. [Re: dIZZY_ALpH]
    #941197 - 10/07/02 10:04 PM (18 years, 9 months ago)

yup, that worked, i have a mark on teh jar to indicate its spread at last checking, and after removing the tape adn flipping them, it has shown marked improvements, spreading more rapidly... i am also raisin the tem as the jars are a bit cold...


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OfflineTeKn0
†hè
Male User Gallery

Registered: 08/30/02
Posts: 801
Loc: ŵįţĥįń ? ?ŧąţĩ...
Last seen: 1 year, 9 months
Re: longest colonization period, EVER. [Re: jahfeelirie]
    #941259 - 10/07/02 10:35 PM (18 years, 9 months ago)

I wouldnt think that 1cc per jar hole is to little.
Ive used 1cc per jar, and had colonization take only 2 weeks.

:confused: 


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Listen, or your tongue will keep you deaf. :wink:
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OfflineUncleCavebear
Dirty ClothesHamper

Registered: 09/23/02
Posts: 24
Last seen: 18 years, 5 months
Re: longest colonization period, EVER. [Re: jahfeelirie]
    #941589 - 10/08/02 03:55 AM (18 years, 9 months ago)

1 cc of solution per hole is a HUGE amount in my opinion (but I'm sure it would work fine). When I am trying to conserve solution, I use just a drop or two per hole; just enough to confirm there is indeed a drop of moisture coming from the syringe. I am able to do approximately 30 4-holed jars with this method, and I would say 97% percent of sites innoculated form mycelium in 3-4 days. These are clear syringes purchased from a vendor as well; no spores visible to the naked eye.

However, if I have solution to spare, I will use more per site to speed colinization time.


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When I was a kid my favorite relative was Uncle Caveman.
After school we'd all go play in his cave, and every once in
a while he would eat one of us. It wasn't until later that I
found out that Uncle Caveman was a bear.


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