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Amazon Shop: ½ Pint Jars, Vermiculite

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Offlinepimster987
journeyman
Registered: 10/02/02
Posts: 73
Last seen: 11 years, 4 months
about sterilizing and incubation
    #934262 - 10/05/02 12:17 PM (14 years, 7 months ago)

ok first when i sterilize with the boiling water method do i still need to put a hole in the lid.and do i put foil o the jar then screw then lid back on then put another peice of foil then steam it.second when incubatin i just incubate through the hole in the lid?cause i was thining wouldnt that cause contamination.or do i close the hole up.


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Offlinecerebral gortex
Prying open my third eye.

Registered: 09/30/02
Posts: 467
Loc: Patriots Nation
Last seen: 6 years, 1 month
Re: about sterilizing and incubation [Re: pimster987]
    #934270 - 10/05/02 12:29 PM (14 years, 7 months ago)

i just used 4 pieces of duck tape to cover the holes during sterilization/incubation period.
make 4 holes in the cover for innoculating.
cover the top of the jar with tin foil
sterilize for about 1 hour
let cool off for at least 3 hours, i let them cool overnight (if there still hot, it will kill the spores)
take the tin foil off, and innoculate thru the four holes, sterilizing the needle after each innoculation (use a lighter to heat up the needle, and let cool for 2-3 minutes in between)
when your done, place tinfoil back on the top of the jars, and place them into your incubator, closet, or wherever you plan to leave them to colonize.

P.S. like i said, i like to use the duck tape, because it adheres to the jars, instead of just being wrapped around it, like tin foil. (and you can boil the jars with the tape on it)
if you plan on handling the jars often to monitor its growth, (like i do!) i would recommend the duck tape.

i hope this helps


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Offlinepimster987
journeyman
Registered: 10/02/02
Posts: 73
Last seen: 11 years, 4 months
Re: about sterilizing and incubation [Re: cerebral gortex]
    #934273 - 10/05/02 12:31 PM (14 years, 7 months ago)

thanks


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InvisibleCheeba
the shroomyqueen

Registered: 09/23/02
Posts: 158
Loc: atop my secret mountain
Re: about sterilizing and incubation [Re: pimster987]
    #934304 - 10/05/02 12:49 PM (14 years, 7 months ago)

my iguana has a tecnique that essentially acts as a barrier...
after he puts his substrate in the jar...he puts a square of foil over and presses down just to the foil is right over the substrate...then he fills the hollow in the foil with vermiculite before he puts the lid on. Then he pressure cooks away...has worked beautifully for him...he's a awsome iguana!!


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Theres one sweet whirld out there!!
Now remember, you are unique! Just like everybody else!


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Amazon Shop: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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