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How to innoculate a myco bag the correct way!
    #933754 -

I've been looking at the posts and browsing around, but every post I see about people starting out with myco bags is they get no growth at all. I've innoculated my bags before and no growth, I've been there, but then I reinjected them the proper way and then 5-7 days the mycelium is spreading.

First of all, let's think about a jar with brown rice substrate. Do you inject the spores directly in the center of the cake, NO! You injected it on the outside of the cake, you should see the spore water come out of the syringe through the glass. SAME THING WITH THE BAGS! You inject it on the outside of the substrate, you should see the needle through the bag, and you should see the water come out of the syringe and spread through the part of the substrate touching the bag. Just make sure you don't puncture the bag or your SOL.


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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #933853 -

cool.

although it seems logical, i assume that most newbies who play with mycobags wont think of it. i think this is a good post. okee. :smile:

Kykeon
athens, greece


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The living ghost of Kykeon

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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #934056 -

What about just pointing the needle straight up and having the spores land on top of the cake?

Also, will room temp work for those bags?

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Re: How to innoculate a myco bag the correct way! [Re: Stymee]
    #934063 -

how much honey water would be used? :confused:


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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #934187 -

Quote:
how much honey water would be used? 




:confused: :confused: :confused: :blush: :grin: :grin: :laugh:

Kykeon
athens, greece 


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The living ghost of Kykeon

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Re: How to innoculate a myco bag the correct way! [Re: kykeon]
    #934823 -

"What about just pointing the needle straight up and having the spores land on top of the cake?"
Dunno never tried it, it could probably still work, but you may need to compress the substrate into a nice rectangular cube.

"Also, will room temp work for those bags?"
depends what kind of strain. search around the message boards. I've read 80-85 degrees is good for colonizing, 75-80 for growing.

"how much honey water would be used?"
What the hell are you talking about? what honey water?


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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #935842 -

just wondering, people who did buy those myco bags, how many of you injected directly into the center of the substrate, and got 0 results?


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Edited by daussaulit (10/06/02 12:25 AM)

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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #936049 -

> "What about just pointing the needle straight up and having the spores land on top of the cake?"
> Dunno never tried it, it could probably still work, but you may need to compress the substrate into a nice rectangular cube.

What? Compress the substrate? Why? In my experience, that just slows down (or kills) mycelium growth.

> "how much honey water would be used?"
> What the hell are you talking about? what honey water?

I believe he's talking about club99's Honeywater Mycelium Tek, or some similar Tek. My guess would be that 5cc's would be adequate.

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Re: How to innoculate a myco bag the correct way! [Re: fungor]
    #937170 -

I had no problem when I compressed the substrate in the bags, they're fully colonized.
As for the honey water tek, didn't use it, so I have no idea.


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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937216 -

well i should corrct myself, there were spores injected as well, i wanted to just toss in as much as possible cause i never have any success with this, when i do it limited because of my sterility....yeah...
but this honey water was good and the spores came with the bags so at least on of them in there should have grown.........


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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #937222 -

did you inject them like I did as described in the 1st post? did you inject about 2cc of spores? Other than that, I'm out of ideas why you can't get it working.


--------------------
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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937248 -

I have ten of those fuckin bags and it has been 2 months. I followed the directions and everything. I injected into the substrate, this is messed up dont u think at least one of them would colonize??

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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #937256 -

There's your problem, are you even reading this post? your aren't suppose to inject into the substrate. Read the 1st post.


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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #937259 -

no injected 2ccs of spore into two of them
a week later i got impatient and tossed the myc. solt'n in all of them
I am writing to customer service and hopefully i'll get 4 new ones
if so, i am gonig to innoc using spores and just leave them next to my furnace, in the box
should be fine right?

What is the consistancy of the bag supposed to be like?
mine is like the seeds are coated in something hard and sticky, they just dont seem to be very hydrated......
when i get new bags, should i put in a few CCs of sterile water to be safe?


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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #937281 -

OK, let me make something extremely clear, if you inject the spores into the bag, and into the substrate, you will get no results, you have to inject it where the bag meets the substrate. You can reinject in the bag with spores and they will be fine if you follow proper sterilization techniques. You can also inject sterilized water into the bag if it looks dry. A rule of thumb that I follow is let the bag sit for a day in about a 85 degree place, and if condensation forms on the bag, your fine, if you don't see any water droplets, then you can add more water.

Also about the bag, if it's inconsistant, you can mix up the bag to make sure that everything is evenly mixed, just don't be too rough or your'll destroy the bag.


--------------------
Beers that I have had.  Total listed:2,302

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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #937285 -

You might think ur not suppose to inject into the substrate. But the directions say that this is how to get best results. :shocked: 

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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #937288 -

where does it say that?


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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937290 -

tahnks for that test using the water droplets, i'm using that from now on....
sterility wasnt an issue when doing these bags as it has been in the past for me...
I mixed the bag around A lot about a week ago cause it hadnt done anything.... i havent stretched or broken the plastic so i assume i wasnt to rough...
a question for these bags, when i get so impatient with this long wait, can i cut open the top, dump out grain, and then sterilize hydrate spawn, seal and pressure coo? I do like to live by the 3 R's....

*edit
about the side thing, i put enough in that some hit the side, some didnt go near it, point is, i should have had something to show for this amount of time waited


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Edited by blinkidiot (10/06/02 06:36 PM)

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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937294 -

It says that at the sporeworks.com

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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #937295 -

As long as it's clean I don't see a problem, but if your going to do that make your own substrate, I'm pretty sure the point of buying bags is because you don't have the supplies and/or time to make your own jars or bags.


--------------------
Beers that I have had.  Total listed:2,302

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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #937297 -

yeah, also see that it says for faster colonization to inject it at the sides?

"Inoculating twice, at opposing angles, towards the sides of the bag is recommended for faster colonization and even inoculant distribution. "


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
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Make your own Wine!

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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937300 -

yeah, but i will be getting new ones....
i want to at least salvage what i can from this attemp, hope fully get a head start on the next bags....
has anybody had overwhelming success with the bags?
I had a pod at one point and it worked well at the fruiting phase for cakes, i got what i payed for there.....these bags seem lacking


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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #937303 -

the online guide on how to use your shroom bags says to inject it directly into the substrate. does anyone live in a dry area using these shroom bags and have a problem with that cuz i think thats what happened to mine.

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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #937308 -

You can reinject the injected failed bag with more spores where the bag is touching the substrate, I did it and it works fine. If you want to see some overwhelming success, look at the grow log form.


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937311 -

re:just wondering, people who did buy those myco bags, how many of you injected directly into the center of the substrate, and got 0 results?

Yes, I am having this problem too what does your substrate look like and how long have you been waiting??

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Re: How to innoculate a myco bag the correct way! [Re: budsmokr]
    #937319 -

"the online guide on how to use your shroom bags says to inject it directly into the substrate."
You must have over looked the part after that, look a few posts up.

"Does anyone live in a dry area using these shroom bags and have a problem with that cuz i think thats what happened to mine."
If you think your bags need a more water, follow my rule of thumb, it's a few posts up. If it's dry, you can inject more water, as described a few posts up.

I'm just wondering guys, are you even reading the posts or do you jump on and post questions that have been answered numerous times?


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #937323 -

You want to see it, look at the grow log form. My grow log can be found here:
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Growlog&Number=875136&page=0&view=collapsed&sb=5&o=&fpart=1


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
Homebrewing(Extract with Specialty Grains)
Homebrewing(All-Grain)
Home wine making
Make your own Wine!

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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937354 -

>OK, let me make something extremely clear, if you inject the spores into the bag, and into the substrate, you will get no results,

I didn?t have that experience. In my case it didn?t really matter since you spread the mycelium anyway through substrate by "massaging" the bag.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: How to innoculate a myco bag the correct way! [Re: Anno]
    #937365 -

That's interesting anno, that's good to know, but from personal experience I didn't have any luck, even though I massaged the bags after a week, there was no mycelium for me. As for everyone else who have been saying the bags are terrible have injected directly into the substrate and got no results after 2 months. Well can you go into more detail about how you injected the bags and how you massaged them?


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
Homebrewing(Extract with Specialty Grains)
Homebrewing(All-Grain)
Home wine making
Make your own Wine!

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Re: How to innoculate a myco bag the correct way! *DELETED* [Re: daussaulit]
    #937373 -

Post deleted by blinkidiot

Reason for deletion: Im sorry



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Re: How to innoculate a myco bag the correct way! [Re: daussaulit]
    #937389 -

what i was wondering was if any1 in an extremely hot, dry enviroment has had problems with these grow bags, i think they may not be working because it isn't humid enough. by the way i live in arizona. is there any way i could get moisture back in the bag and get the mushroooms to grow in the same bag as before.

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Re: How to innoculate a myco bag the correct way! [Re: blink]
    #937398 -

>massaged using thumb and full palm
And some massage oil :wink:

No, like you described it.
i inoculated through a alcohol swab cleaned bag wall, and i put a tape over it afterwards. Stored at 80?F. 

Results: http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum2&Number=620143 .


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

Edited by Anno (10/06/02 07:13 PM)

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Re: How to innoculate a myco bag the correct way! [Re: budsmokr]
    #937408 -

Can you not read? Did you bother to read all the posts on this thread? Look at the post that was posted "10/07/02 05:29 AM", your question has already been answered.


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
Homebrewing(Extract with Specialty Grains)
Homebrewing(All-Grain)
Home wine making
Make your own Wine!

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Re: How to innoculate a myco bag the correct way! [Re: KrazyColors]
    #938639 -

One more thing, if you guys are impatient like me and don't want to wait long for results, why choose the longer method and inject into the substrate? It says to inject it at the edges for faster results.


--------------------
Beers that I have had.  Total listed:2,302

Make your own beer!
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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