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monkiman
human being


Registered: 06/30/10
Posts: 1,030
Loc: somewhere in space time.
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13442153 - 11/06/10 03:41 AM (13 years, 2 months ago) |
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flowhood? i build ducting for extraction systems as one of my jobs so i suppose i could whip something similar up. does it do away with a glove box if you have one of these?
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total
Post Office Tyvek Advocator




Registered: 10/03/04
Posts: 11,405
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: monkiman]
#13442241 - 11/06/10 05:32 AM (13 years, 2 months ago) |
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A glovebox still has its uses... For example... If you have a badly contaminated plate, you want to work in a glove box.... that way your flow hood isnt blowing the contams around off the plate... still air would be better in that case...
Sorry for sounding stupid atm...its late...and
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amungus
Scag Baron




Registered: 10/12/10
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13443952 - 11/06/10 03:25 PM (13 years, 2 months ago) |
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hi , i was wondering if anyone could help me out , i ordered some jars and filters from mushbox and they sent filters that were too small , is there anyway i can rig that , or do i need to just get some more filters that fit,this is the only thing keeping me from starting up, thank you
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     "Seems so sick to the hypocrite norm Running their boring drills But we are an elite race of our own The stoners, junkies, and freaks" - Layne Staley
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total
Post Office Tyvek Advocator




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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: amungus]
#13444017 - 11/06/10 03:40 PM (13 years, 2 months ago) |
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You should contact them in the Sponsor's Forum You'll get a nice quick speedy response there! Goodluck
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amungus
Scag Baron




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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13444529 - 11/06/10 05:16 PM (13 years, 2 months ago) |
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thanks man
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     "Seems so sick to the hypocrite norm Running their boring drills But we are an elite race of our own The stoners, junkies, and freaks" - Layne Staley
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MartincountyYahoo
Wasted Rock Ranger



Registered: 10/20/10
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: 13shrooms]
#13445855 - 11/06/10 10:25 PM (13 years, 2 months ago) |
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Got treasure coast syringe over a week now since enoculating am i screwed does it take long for colonization B+ was like 5 days someone help a cracker out
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monkiman
human being


Registered: 06/30/10
Posts: 1,030
Loc: somewhere in space time.
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13446389 - 11/07/10 12:51 AM (13 years, 2 months ago) |
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hi there, i just inoculated my jars and have few questions. should i now tighten the jar lids?(im doing rubber side up) the micro-pore tape was very sticky when i was taking it off to inoculate, i had a hard time getting it off and ended up putting new tape on after i inoculated, should i just leave the tape on in the early stages of incubation?(i have a dry verm layer) also i might have done a few holes twice as in 2ml instead of 1ml does this matter?
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total
Post Office Tyvek Advocator




Registered: 10/03/04
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: monkiman]
#13446536 - 11/07/10 01:36 AM (13 years, 2 months ago) |
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Id love to answer your questions! 
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should i now tighten the jar lids?(im doing rubber side up)
You should be good, as long as the lid is on there holding your contents in...i assume your doing BRF because of you talking about the verm layer?
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the micro-pore tape was very sticky when i was taking it off to inoculate, i had a hard time getting it off and ended up putting new tape on after i inoculated, should i just leave the tape on in the early stages of incubation?(i have a dry verm layer)
Micropore tape is abit overkill on BRF jars...the vermiculite layer you speak of acts as your contam barrier...your acctually inhibiting gas exchange... On your next batch, try it without it...I bet you get just as good results, and save some $ on micropore and avoid messing with it's stickyness 
Quote:
also i might have done a few holes twice as in 2ml instead of 1ml does this matter?
If i recall correctly when i did PF-Tek, it called for 4 holes around the outside of your jar... In each hole the directions were to put 1/4cc/ml into each hole, for a combined total of 1cc/ml per jar... If you put 2cc/ml per 4 holes, that 8cc/ml per jar.... You did tooo much man...tooo much
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monkiman
human being


Registered: 06/30/10
Posts: 1,030
Loc: somewhere in space time.
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13446577 - 11/07/10 01:54 AM (13 years, 2 months ago) |
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cool picture, i must have read wrong that it was 1cc per hole, actually now that i think about it was probably more like .5cc/ml per hole that i used, because just by looking at the solution coming out i figured that 1cc was a bit much.my brf mix was pretty dry to start off with and i dont notice any water collecting at the bottom of the jar so i will have to wait and see.i am going to remove the tape now too. thanks for all the help.
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total
Post Office Tyvek Advocator




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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: monkiman]
#13446589 - 11/07/10 01:03 AM (13 years, 2 months ago) |
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Quote:
monkiman said:
cool picture, i must have read wrong that it was 1cc per hole, actually now that i think about it was probably more like .5cc/ml per hole that i used, because just by looking at the solution coming out i figured that 1cc was a bit much.my brf mix was pretty dry to start off with and i dont notice any water collecting at the bottom of the jar so i will have to wait and see.i am going to remove the tape now too. thanks for all the help.
Glad I could help! I wouldnt worry about using TOO many spores  There are disadvantages to it, but none that are going to shine thru on a n00b cube grow  As for the tape...I wouldnt both to "remove" it...
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Dragonaut


Registered: 06/24/04
Posts: 6,190
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13447550 - 11/07/10 09:22 AM (13 years, 2 months ago) |
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Quote:
total said: If you put 2cc/ml per 4 holes, that 8cc/ml per jar.... You did tooo much man...tooo much
That's a bunch of BS. Works fine, maybe even better. Especially say, if your jars were prepped on the dry side, or sat for an extended period (more than 2 weeks) uninoculated. As long as you have enough spore solution...go nuts. Your jars just shouldn't have any standing water pooled in the bottom. More spore water works better, particularly if your solution is light.
Edited by Dragonaut (11/07/10 09:29 AM)
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total
Post Office Tyvek Advocator




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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: Dragonaut]
#13448091 - 11/07/10 11:53 AM (13 years, 2 months ago) |
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Quote:
Dragonaut said:
That's a bunch of BS. Works fine, maybe even better. Especially say, if your jars were prepped on the dry side, or sat for an extended period (more than 2 weeks) uninoculated. As long as you have enough spore solution...go nuts. Your jars just shouldn't have any standing water pooled in the bottom. More spore water works better, particularly if your solution is light.
I can see the dry side...But just let me say, that Dragonaut is completely wrong...Ill post some quotes from some trusted cultivators
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Cervantes said: Anything more than 2cc in a half pint jar is really overkill. Those jars are small. They will colonize. Hell, 5 drops will work. A quart of grain will colonize in a week to 10 days with .5-1cc injected.
Also, you will get many conflicting substrains growing in your jar if you use a lot of spores. This can sometimes cause stalled cakes or uneven growth... but that isn't usually a problem.
Cakes don't need that much water. The more cc's of water you use, the more likely you are to inject contaminants.
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mycofile said: I've personally noticed that darker syringes aren't better for three reasons. 1) dark sryinges have large clumps of spores which make them visible. I've noticed that syringes with more evenly distributed spores provide better results. Some actually blend their spore solution, and add stabilizers to keep the spores from clumping. This results in a syringe which isn't as dark, but which is "hot" meaning no dead inoculation points. and 2) If it's not a matter of large clumps of spores, but of total spore load in a syringe, I don't think the more spores the merrier. In fact, I've noticed that having too many spores produces more tomentose/fluffy mycelium than rhizomorphic mycelium. This IMO not only slows growth, but hinders fruiting. Apparently, up to a certain spore load, the rhizomorphic strains quickly outrace the tomentose ones. But increase the spore load too much and there are more tomentose strains that the rhizomorphs have a harder time outracing. As we all know rhizo's grow faster and fruit better than fluffies. 3) This one's not quite as important, it's theoretical and not based on direct observance, rather deduction. The darker the syriges, the more spore prints used. The more spore prints used the higher the threat of contamination. Also, to a certain extent, the more prints a vendor needs, the higher his overhead which may be passed on to consumers or simply lead to vendor disgruntlement at aquiring all those prints.
There are just two copy and pastes...you can UTFSE yourself and see that too much spores are generally a BAD THING... Please disregard Dragonaut's post... (Not hating on you bro, your just tellin the n00b bad info)
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: Dragonaut]
#13448414 - 11/07/10 01:04 PM (13 years, 2 months ago) |
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Quote:
Dragonaut said:
Quote:
total said: If you put 2cc/ml per 4 holes, that 8cc/ml per jar.... You did tooo much man...tooo much
That's a bunch of BS. Works fine, maybe even better. Especially say, if your jars were prepped on the dry side, or sat for an extended period (more than 2 weeks) uninoculated. As long as you have enough spore solution...go nuts. Your jars just shouldn't have any standing water pooled in the bottom. More spore water works better, particularly if your solution is light.
Dragonaut, I thought you knew better. 
if your using LC for inoculation then yeah go nuts as long as you dont fudge up the moisture content of your sub/grain. but when it comes to ms inoculations less is more. 
its about the genetic variety that happens with MS as opposed to inoculating with a isolation/monoculture. 
they may be the same substrain using MS but they still compete so to speak for area/food and you might not get as many compatible knots as you would have using iso/mono cultures. 
Im pretty baked so I hope that came out through my fingers correctly
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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monkiman
human being


Registered: 06/30/10
Posts: 1,030
Loc: somewhere in space time.
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: 13shrooms]
#13451263 - 11/07/10 10:34 PM (13 years, 2 months ago) |
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the print that i used was quite light and on paper and it was pretty small too (20mm), i made about 8 syringes out of it(my first time making syringes).the spore solution was very clear but i could see spores in it. so i did not understand all the technical talk above, but would i be right in thinking that if the spore solution has less spores the area /food thing would not be an issue because there is more water but a lot less spores than a dark syringe. i could also be talking shit as i am a noob and i
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total
Post Office Tyvek Advocator




Registered: 10/03/04
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: monkiman]
#13451319 - 11/07/10 10:47 PM (13 years, 2 months ago) |
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Its really nothing too bad... There are 2 things that worry me about mass usage of spore solution.
1. More spores having ....So more substrains 2. More usage of home made media...More potential for contams
#1 doesnt really shine through in cubes though...so you should be good!
goodluck total
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monkiman
human being


Registered: 06/30/10
Posts: 1,030
Loc: somewhere in space time.
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13451427 - 11/07/10 11:13 PM (13 years, 2 months ago) |
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thanks total, i will update in a few days.
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misfit78
Critter



Registered: 06/07/10
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: monkiman]
#13458085 - 11/09/10 09:39 AM (13 years, 2 months ago) |
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ok i sealed a filter patch bag with a lighter will it be ok
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total
Post Office Tyvek Advocator




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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: misfit78]
#13458801 - 11/09/10 12:43 PM (13 years, 2 months ago) |
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Quote:
misfit78 said: ok i sealed a filter patch bag with a lighter will it be ok 
Did you do this before/after sterilization? Id suggest just tying/rubberbanding it... That way you can reuse your bags as well
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misfit78
Critter



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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: total]
#13463053 - 11/10/10 09:17 AM (13 years, 2 months ago) |
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before sterilization?
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hamloaf
Loaf of Fam.

Registered: 12/23/09
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Re: The Shroomery and mushroom cultivation, a newcomers guide. [Re: misfit78]
#13463071 - 11/10/10 09:24 AM (13 years, 2 months ago) |
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Quote:
misfit78 said: before sterilization?

If you don't have a sealer, you can use a curling iron or a clothing iron to seal bags with.
If you were to seal the bags after sterilization, unless you had a flowhood, used tyvek wrist sleeves or some other form of seal less measure to sterilize your bags, how would the bags stay sterile?
Edited by liquidmyce (11/10/10 12:06 PM)
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