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OfflineDISCGOLFNDOSE
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Registered: 09/16/02
Posts: 167
Loc: International Waters (Oil...
Last seen: 20 years, 9 months
Agar ???? Help
    #928157 - 10/03/02 10:06 AM (21 years, 8 months ago)

What would ? need to add to this VVV to make agar for petri dishes??







Should ? follow this recipe??

Malt Extract Agar (MEA) solution that is shipped to me in a powderform. Mix the agar solution according to the directions:

-Mix 50 grams of MEA (7 Tablespoons) for each liter of water.
-Autoclave (pressure cook/can) at 15psi for 45 minutes.
-Allow the MEA solution to cool to 150 degrees before pouring .


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Leave me alone I'm a Botanist not a Mycologist.

gRiP-n-RiP


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OfflineDERRAYLD
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Registered: 05/13/02
Posts: 10,427
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Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928162 - 10/03/02 10:08 AM (21 years, 8 months ago)

make up a batch of pda to use as a nutrient base for the agar.

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928166 - 10/03/02 10:09 AM (21 years, 8 months ago)

Yes follow directions , if you want to make less , just divide the recipe in equal parts . :cool: 


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OfflineDISCGOLFNDOSE
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Registered: 09/16/02
Posts: 167
Loc: International Waters (Oil...
Last seen: 20 years, 9 months
Re: Agar ???? Help [Re: tripndicular]
    #928193 - 10/03/02 10:19 AM (21 years, 8 months ago)

This bag of malt extract dosn't have all of the nutrients needed??


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Leave me alone I'm a Botanist not a Mycologist.

gRiP-n-RiP


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OfflineDERRAYLD
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Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928205 - 10/03/02 10:23 AM (21 years, 8 months ago)

the malt extract should be fine

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OfflineDISCGOLFNDOSE
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Registered: 09/16/02
Posts: 167
Loc: International Waters (Oil...
Last seen: 20 years, 9 months
Re: Agar ???? Help [Re: DERRAYLD]
    #928222 - 10/03/02 10:31 AM (21 years, 8 months ago)

So does the malt extract need agar also or not VVVV??

These recipes are designed for agar maintenance of Psilocybe species. All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure. 500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.

Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water



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Leave me alone I'm a Botanist not a Mycologist.

gRiP-n-RiP


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Invisiblezeta
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Registered: 05/24/02
Posts: 3,972
Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928227 - 10/03/02 10:33 AM (21 years, 8 months ago)

Yes you will need to add agar to that.
MEA contains malt extract and agar, you could also add some yeast extract to make MYA


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