Home | Community | Message Board


High Mountain Compost
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Autoclave, Malt Extract, Petri Dish, pH Test Strips, Potato Dextrose

Jump to first unread post. Pages: 1
OfflineDISCGOLFNDOSE
Chain Ringer
Registered: 09/16/02
Posts: 167
Loc: International Waters (Oil...
Last seen: 15 years, 4 days
Agar ???? Help
    #928157 - 10/03/02 12:06 PM (15 years, 10 months ago)

What would ? need to add to this VVV to make agar for petri dishes??







Should ? follow this recipe??

Malt Extract Agar (MEA) solution that is shipped to me in a powderform. Mix the agar solution according to the directions:

-Mix 50 grams of MEA (7 Tablespoons) for each liter of water.
-Autoclave (pressure cook/can) at 15psi for 45 minutes.
-Allow the MEA solution to cool to 150 degrees before pouring .


--------------------
Leave me alone I'm a Botanist not a Mycologist.

gRiP-n-RiP



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
Last seen: 9 years, 9 months
Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928162 - 10/03/02 12:08 PM (15 years, 10 months ago)

make up a batch of pda to use as a nutrient base for the agar.


--------------------
Derrayld and out of control


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928166 - 10/03/02 12:09 PM (15 years, 10 months ago)

Yes follow directions , if you want to make less , just divide the recipe in equal parts . :cool: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDISCGOLFNDOSE
Chain Ringer
Registered: 09/16/02
Posts: 167
Loc: International Waters (Oil...
Last seen: 15 years, 4 days
Re: Agar ???? Help [Re: tripndicular]
    #928193 - 10/03/02 12:19 PM (15 years, 10 months ago)

This bag of malt extract dosn't have all of the nutrients needed??


--------------------
Leave me alone I'm a Botanist not a Mycologist.

gRiP-n-RiP



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
Last seen: 9 years, 9 months
Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928205 - 10/03/02 12:23 PM (15 years, 10 months ago)

the malt extract should be fine


--------------------
Derrayld and out of control


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDISCGOLFNDOSE
Chain Ringer
Registered: 09/16/02
Posts: 167
Loc: International Waters (Oil...
Last seen: 15 years, 4 days
Re: Agar ???? Help [Re: DERRAYLD]
    #928222 - 10/03/02 12:31 PM (15 years, 10 months ago)

So does the malt extract need agar also or not VVVV??

These recipes are designed for agar maintenance of Psilocybe species. All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure. 500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.

Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water



--------------------
Leave me alone I'm a Botanist not a Mycologist.

gRiP-n-RiP



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: Agar ???? Help [Re: DISCGOLFNDOSE]
    #928227 - 10/03/02 12:33 PM (15 years, 10 months ago)

Yes you will need to add agar to that.
MEA contains malt extract and agar, you could also add some yeast extract to make MYA



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Autoclave, Malt Extract, Petri Dish, pH Test Strips, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* agar-and nutrient or just ..the nutrient i found. Ass_Pirate 293 0 05/11/05 09:28 PM
by Ass_Pirate
* Agar? Maddoc 862 2 07/15/01 10:47 PM
by aluminum_can
* might be an advance agar question
( 1 2 all )
fugu 5,818 33 02/05/03 08:45 AM
by fugu
* Potato Dextrose Agar X Malt Extract Agar Alegalize 2,270 8 02/25/05 01:03 PM
by agar
* right malt extract? scrname 1,082 11 04/12/05 09:35 AM
by ohmatic
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 2,782 8 02/14/05 09:57 AM
by Roadkill
* test tubes agar fungophiliac 1,277 7 12/13/04 11:34 PM
by fungophiliac
* Will spores germinate in agar? MycoJunkie 6,473 10 04/01/05 02:28 PM
by derx

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
814 topic views. 24 members, 88 guests and 15 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
SoulSpeciosa Kratom
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.006 seconds on 19 queries.