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 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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 LateForTheFuture
Old Hand
 
Registered: 02/24/03
Posts: 845
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 Re: incoulation Time !  [Re: J3illy]
#9265740 - 11/18/08 12:42 PM (15 years, 4 months ago) |
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It's not misleading, as its TRUE!
In fact, when I once was a noob, I didn't have the same resources I have now, and I NEVER, to this day, flame sterilized!
We can argue to the death here, but I'm the one who's done it and knows it works, so why are you arguing?
Again, a chirping bird, your wasting this post and its getting wayyy off topic, why dont you start your own debate thread about this and stop arguing and let this guy, or any other "noob", read it, and they shouldn't have a problem.
All I have to say is : I've never used flame sterilization and never will!  Everyone goes about things differently here, and I gave my input of how I go about things, and its pretty understandable to anyone, even "noobs", who read my post what I was getting at. Why did I edit it immediately after posting? Because I knew some kid like you would try to make a big debate about flame sterilization.
My post is in NO WAY misleading, because in the past, I didn't flame sterilize and colonization went on fine! And this was with "dirty" "used" needles, which I boiled and filled with boiling water. Maybe I'm a clean guy, and I naturally fight off all molds in my house! HAH.
In fact, my post is quite helpful, and your posts are making this thread a big pain in the ass. By the way, where the hell did you get that 10 minute number? Maybe the doctor should let my skin sit for 10 minutes after he wipes it to make sure its sterile, huh?
Bloody hell.
Can someone give me some pot? Woo.
Good day, I hear the birds chirping...
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure 
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Re: incoulation Time ! [Re: J3illy]
#9265774 - 11/18/08 12:48 PM (15 years, 4 months ago) |
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what late is trying to say is
"if you use a sterile needle fresh from the wrapper every time"
there is no need to flame the needle
-noobie-
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AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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OK 1st of all you're giving info that's completely contradictory to what everyone does - as EVERYONE flame sterilized their needles. If it's brand new, it's obviously not necessary. If you're using it after that, you SHOULD flame sterilize it, and suggesting someone NOT to is just wrong.
And alcohol is NOT a sterilizer, it's a sanitizer. It has SOME sterilizing ability, but there are many things it will not kill like mold/endospores. And to even be effective as a partial sterilizer, it needs time to do it. If you don't believe me, go read up on it some more.
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 The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 7 months, 21 days
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Re: incoulation Time ! [Re: J3illy]
#9265797 - 11/18/08 12:52 PM (15 years, 4 months ago) |
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OOps how did that happen.
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AMU Q&A thread.
Edited by The shroomy 1 (11/18/08 12:53 PM)
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LateForTheFuture
Old Hand
Registered: 02/24/03
Posts: 845
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Re: incoulation Time ! [Re: J3illy]
#9265801 - 11/18/08 12:53 PM (15 years, 4 months ago) |
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RR, someone, lock this shit before I become a heated bitch.
I've only had two times where Ive had problems on this forum:
Arguing with someone about "hydroponic mushrooms"
and you.
IGNORE.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Quote:
noobieshroomie said:
what late is trying to say is
"if you use a sterile needle fresh from the wrapper every time"
there is no need to flame the needle
I completely agree with this, but I'm just saying the way he posted it with, "I have never used flame sterilization and find it pointless if you're using rubbing alcohol." at the top of the post - that could easily be interpreted by a n00b that they never have to flame sterilize. Not everyone uses a new needle every time.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Quote:
LateForTheFuture said:
and you.
IGNORE.
You're the one causing all this drama! All I said was his point was misleading, and he rates me a 0 then starts sending me PM's telling me I'm "beefing" with him.
You're point was misleading!
The majority of people will not be using a brand new needle every time - so flame sterilizing is not going to be "pointless" as you say, it's going to be necessary.
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: incoulation Time ! [Re: J3illy]
#9265860 - 11/18/08 01:06 PM (15 years, 4 months ago) |
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it was i little misleading but what he IS saying is with
a new needle you do not need to flame it just wipe it down with alcohol he does not mention reusing any of the needles
-noobie-
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AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 7 months, 21 days
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Re: incoulation Time ! [Re: J3illy]
#9265878 - 11/18/08 01:10 PM (15 years, 4 months ago) |
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Quote:
J3illy said:
OK 1st of all you're giving info that's completely contradictory to what everyone does - as EVERYONE flame sterilized their needles. If it's brand new, it's obviously not necessary. If you're using it after that, you SHOULD flame sterilize it, and suggesting someone NOT to is just wrong.
And alcohol is NOT a sterilizer, it's a sanitizer. It has SOME sterilizing ability, but there are many things it will not kill like mold/endospores. And to even be effective as a partial sterilizer, it needs time to do it. If you don't believe me, go read up on it some more.
PERFECTLY PUT! You don't have to explain yourself anymore J3illy You contributed well to this community with the above statement.
The Shroomy 1
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AMU Q&A thread.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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LateForTheFuture
Old Hand
Registered: 02/24/03
Posts: 845
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Quote:
LateForTheFuture said:
I have never used flame sterilization and find it pointless if you're using rubbing alcohol. I don't ever see my doctor busting out the flame for my injections.
I just keep a wet paper towel of rubbing alcohol and keep the syringe tip wrapped in that, ensuring I quickly wrap it back up after each injection.
Just my experience. Not bashing it or anything, just found I've never had to do it, so don't get too concerned about it.
But, hey, do what works for you! 
"Obviously the safest way would be to flame it and wipe it w/ alcohol before its stuck into any innoc point, same jar or not... Flaming is for sterilizing the inside hollow portion of the needle, alcohol is to sterilize the outside of the needle and to cool it after flaming... With that knowledge, do what you thinks best for your particular situation... GL"
I'm just going to repost what I said, for some more MISINFORMATION and to clear things up.
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Joker003
Lets put a Smile on that Face !
Registered: 10/13/08
Posts: 130
Loc: USA
Last seen: 15 years, 9 days
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Jacking my therad to fight like bitcheez,
get over it Shit
its not even that big
of a deal ! 
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Lets put a Smile on that Face !
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Joker003
Lets put a Smile on that Face !
Registered: 10/13/08
Posts: 130
Loc: USA
Last seen: 15 years, 9 days
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Re: incoulation Time ! [Re: Joker003]
#9267679 - 11/18/08 05:56 PM (15 years, 4 months ago) |
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if you want to fight and complain at each other do it on your own post dont jack one to argue about a lil stupid problem. 
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Lets put a Smile on that Face !
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: incoulation Time ! [Re: Joker003]
#9267687 - 11/18/08 05:57 PM (15 years, 4 months ago) |
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The 1st post got your point across, thanks.
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Re: incoulation Time ! [Re: Joker003]
#9267697 - 11/18/08 05:59 PM (15 years, 4 months ago) |
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And anyway, recycling is good for the environment! It hurts the nature to be wasting needles like that, getting a new needle for each inoculation... go green and recycle your needles - flame them! Inoculation needles don't need to be sharp, so you can use the same needles for the rest of your life!
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
 Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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holyongos
anarchy is the way of the future
Registered: 09/28/08
Posts: 80
Loc: land of the morons, I mea...
Last seen: 6 years, 4 months
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Re: incoulation Time ! [Re: Joker003]
#9267755 - 11/18/08 06:05 PM (15 years, 4 months ago) |
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Quote:
Joker003 said:
i started a Fire in my Glove boX ! It was crazy and it scared me. LOL it was like a mini Fire ball in there
I've donethe same thing. alcohol fumes caught and burnt all the hair off my arms. it was a still air not a glove box
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I ate something funny, or at least thats how it made me feel about it.
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Joker003
Lets put a Smile on that Face !
Registered: 10/13/08
Posts: 130
Loc: USA
Last seen: 15 years, 9 days
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Re: incoulation Time ! [Re: holyongos]
#9267805 - 11/18/08 06:12 PM (15 years, 4 months ago) |
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Quote:
holyongos said:
Quote:
Joker003 said:
i started a Fire in my Glove boX ! It was crazy and it scared me. LOL it was like a mini Fire ball in there
I've donethe same thing. alcohol fumes caught and burnt all the hair off my arms. it was a still air not a glove box
lol i kno, it scared the shit out of me ! my arms also got there hair brunt off lol. so i continued it in the same room just not in the box lol. i am a lil worryed becouse it was my 1st time and things didnt go to good with the plan or the box. i was really nervous and it was hard to keep steady and still. i made 5 jars and i hope to see all 5 fruit, but im no expert and i had not one to help me excpet the help form the Good comunity of the shroomery and resarech. so hope i at least get 1 to colinzed and if im lucky then all 5 will. 
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Lets put a Smile on that Face !
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holyongos
anarchy is the way of the future
Registered: 09/28/08
Posts: 80
Loc: land of the morons, I mea...
Last seen: 6 years, 4 months
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Re: incoulation Time ! [Re: Joker003]
#9268371 - 11/18/08 07:35 PM (15 years, 4 months ago) |
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I'm still quite the novice myself. alot of trial and error but doing it constantly for over a year now i'm starting to get the hang of things. i like to use alcohol a lot when knocking up jars. when i started i only flamed and got more contams. or maybe i've just been getter better with my sterilizing, I couldn't say. but I support alcohol use and abuse.
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I ate something funny, or at least thats how it made me feel about it.
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