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OfflineShroomer Smurf
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Registered: 11/02/08
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Used Syringe Sterilization Procedure?
    #9256942 - 11/16/08 10:31 PM (12 years, 3 months ago)

Hi fellas, I have some LC in the works and want to use my old spore syringe for the liquid culture. Whats the best way to sterilize the used syringe? Can it be PC'd?


Thanks In Advance. :stoned:


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InvisibleJ3illy
Trainee

Registered: 10/18/08
Posts: 3,344
Re: Used Syringe Sterilization Procedure? [Re: Shroomer Smurf]
    #9256945 - 11/16/08 10:32 PM (12 years, 3 months ago)

Yes.


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Offlinelittlediddy2001
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Registered: 03/18/08
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Re: Used Syringe Sterilization Procedure? [Re: Shroomer Smurf]
    #9256956 - 11/16/08 10:33 PM (12 years, 3 months ago)

Wrap it in tin foil and PC it.

However, syringes cost less than $1. A new one would be best.


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InvisibleJ3illy
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Registered: 10/18/08
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Re: Used Syringe Sterilization Procedure? [Re: littlediddy2001]
    #9256989 - 11/16/08 10:39 PM (12 years, 3 months ago)

Yea you can order a 100 pack for $25 on www.themadseason.com
18g 1.5"


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Offlinej.guit.err
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Registered: 10/17/08
Posts: 213
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Re: Used Syringe Sterilization Procedure? [Re: J3illy]
    #9257072 - 11/16/08 10:53 PM (12 years, 3 months ago)

I pick mine up at a pharmacy, never really had any questions either. Except at target...bastards.


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OfflineElixier
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Registered: 11/16/08
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Re: Used Syringe Sterilization Procedure? [Re: j.guit.err]
    #9257173 - 11/16/08 11:13 PM (12 years, 3 months ago)

I put mine in foil and pressure cook at 15 psi for 20 minutes.  I have heard you can boil them, can anyone confirm this and provide a simple writeup if true?


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Invisiblemusher_420
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Registered: 08/01/05
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Re: Used Syringe Sterilization Procedure? [Re: Elixier]
    #9257225 - 11/16/08 11:23 PM (12 years, 3 months ago)

Boiling works.  You can also suck up a few mL and then microwave them.  I always just PC them tho because it's pretty much just as easy as boiling and is 100% sure to be effective.

To boil I used to bring a pot of water to boil then I'd throw in my syringes with the needles attached and the plunders depressed.  I'd let them boil for about 5 minutes then suck up some water from the pot then squirt that into the sink just to clean the inside a bit.  I'd then throw them back into the water for another 10-20 minutes.  I'd put them into a large freezer zip-lock bag while they were still hot.


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OfflineVermonster420
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Re: Used Syringe Sterilization Procedure? [Re: Elixier]
    #9257230 - 11/16/08 11:25 PM (12 years, 3 months ago)

I just half-fill them with distilled h2O, wrap in foil neatly sealed seams, PC with my jars.  store unopened in fridge until used (butter compartment has been sanitized).


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Offlinebootedboy
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Re: Used Syringe Sterilization Procedure? [Re: Vermonster420]
    #9257275 - 11/16/08 11:33 PM (12 years, 3 months ago)

I boil some filtered tap water, like Brita, for about six to ten minutes and then suck some up in the syringe. Allow the hot water to sit in the syringe for two minutes and then shoot it in the sink. I then suck up water and immediately shoot it in the sink two to three more times. Finally I suck up some water and allow the water to cool. This is how I make spore syringes and it provides a sterile syringe and sterile water. This method could be used for LC, just shoot out the water just prior sucking up your LC and flame sterilize the needle after removing the water. This method would help block contaminants from entering the main body of the syringe. It never fails for me.


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Offlineveda_sticks
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Re: Used Syringe Sterilization Procedure? [Re: bootedboy]
    #9258612 - 11/17/08 07:28 AM (12 years, 3 months ago)

Quote:

bootedboy said:
I boil some filtered tap water, like Brita, for about six to ten minutes and then suck some up in the syringe. Allow the hot water to sit in the syringe for two minutes and then shoot it in the sink. I then suck up water and immediately shoot it in the sink two to three more times. Finally I suck up some water and allow the water to cool. This is how I make spore syringes and it provides a sterile syringe and sterile water. This method could be used for LC, just shoot out the water just prior sucking up your LC and flame sterilize the needle after removing the water. This method would help block contaminants from entering the main body of the syringe. It never fails for me.




I have successfully used this method for cleaning syringes prior to using LC.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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