To make the manure extract just soak it in hot water for a couple hours, and then take that water and replace it with water in the making of both agar, and liquid innoculant.

Manure!=store bought. Go into a field and get it yourself. All my experiments with store bough failed miserably... why? Because it didn't have real straw in it, probably, it had all been finely screened.

aloha...BiO.

I got one more question for you... well 2 actually..

1. Do you process your manure in any way, ie pasteurize, sterilize, etc.

2. Why do you think the liquid works when you use manure water? My guess is that you are training the mycelium... Stamets mentions something like this in GGMM... The big vats of liquid innoculant contain some of the future substrate, so as to prepare them... I wonder if the manure water gives an advantage to strains capable of growing on the manure easily. Hmmm. I will have to check this out...

ThE JafF

DD

This was a very pesky strain for me to grow. I'm used to doing agar transfers without modification with Ps. Cubs when I started working with them. I found much to my chagrine that they really didn't like malt by itself. This took quite a bit of experimenting and quite a few fuck this I'm quiting type of attitudes, but finally I got it. They just want a bit of manure in EVERYTHING... weird, but that's been successful in my work.

Have fun jaffster...