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Offlinetheidiotnoob
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transplanting PF jars to Rye....
    #9228837 - 11/12/08 06:26 AM (15 years, 4 months ago)

Having really hard time getting PF jars to colonize quickly. It has been about 6 weeks and only 2 out of 15 jars are 100%. A few days ago I took a few of the jars, cracked them open and removed the Mycelium and transplanted it into qt. jars of rye and they immediately took off. I mean took off! today my rye jars are fully colonized. No contam or anything. All books I read on Mycology stated that you should do PF tek a few times b4 attempting bulk. Although I have not grown a single "one" yet, I find that it is WAY more exciting looking at grain jars going so fast, rather than watching PF jars do barely anything. I hope I have as much luck spawning as I have with Rye. I have  10 or so bulk jars


--------------------
If I were a dog,
And Trich were a flower,
I would lift my leg,
And give them a shower :grin:

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Offlinedead
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Registered: 08/28/08
Posts: 2,761
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Re: transplanting PF jars to Rye.... [Re: theidiotnoob]
    #9228844 - 11/12/08 06:30 AM (15 years, 4 months ago)

Wait, you took an uncolonized PF cake out from it's jar?

:banghead:

Don't do that. You were very very very lucky not to get contaminations.


--------------------
"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."

Links:
:regularshroom:Nibin's Guide for Noobs
:regularshroom:some easy teks on bulk & grain prep. (my journal)

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Offlineveda_sticks
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Re: transplanting PF jars to Rye.... [Re: dead]
    #9228852 - 11/12/08 06:33 AM (15 years, 4 months ago)

Doesnt sound good, 2 days colinisation time is far to fast for colinisation. Even from doing a grain to grain.

If your pf teks are taking ages to colinise. your doing something wrong.

overly wet substrate
Incubation temps to hight
no gas exchange
contamination


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinetheidiotnoob
skedooosh!
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Registered: 09/23/08
Posts: 53
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Re: transplanting PF jars to Rye.... [Re: veda_sticks]
    #9228867 - 11/12/08 06:43 AM (15 years, 4 months ago)

Basically, I removed the cakes at about 75%. There was no growth in them for over a week. I figured that rather than wait for contam to show up i would experiment. I put it in my glove box and cut away the mycelium. Then transferred to rye. It has been like 4 days now and they seem to be taking right off, big time. I cut the mycelium into like 1cm pieces and put them and required peroxide into jars. Incubated at 80 degrees, they seem to be doing fine. I have to think that an uncolonized pf jar would contam after 6wks of growth so figured I would have nothing to lose, right?


--------------------
If I were a dog,
And Trich were a flower,
I would lift my leg,
And give them a shower :grin:

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Offlinedead
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Re: transplanting PF jars to Rye.... [Re: theidiotnoob]
    #9228879 - 11/12/08 06:50 AM (15 years, 4 months ago)

You could have just removed the uncolonized parts and fruited it.

A cake not colonizing properly is usually either not enough GE, too much FAE (starts pinning) or bacteria. If it's bacteria, congrats you just transfered bacteria to your grain...


--------------------
"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."

Links:
:regularshroom:Nibin's Guide for Noobs
:regularshroom:some easy teks on bulk & grain prep. (my journal)

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Offlineveda_sticks
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Re: transplanting PF jars to Rye.... [Re: dead]
    #9228884 - 11/12/08 06:52 AM (15 years, 4 months ago)

I would expect to see alot of metobolites if there was bacteria present.

Quote:

A large amount of metabolites generally means a bit of bacteria in the jar. The metabolites are antibiotic secretions, so when they're in large amounts, it usually means something is up. High colonization temps will also lead to metabolite production, so remember to colonize jars at room temperature.

A jar with a good filter will contain any contaminants that are within, so don't toss it out. Don't use any jar with a lot of metabolites for grain to grain transfers, but if they colonize fine, they're good to use for spawning to bulk.
RR




--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineArtex
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Re: transplanting PF jars to Rye.... [Re: theidiotnoob]
    #21369579 - 03/06/15 07:25 AM (9 years, 15 days ago)

I'm hoping you're still active on this forum.  What was the result of this experiment?

I'm considering using some of my successful PF Tek mycelium to transfer to one of my grain jars as an experiment, but I would prefer not to waste time and space (pun intended).

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: Artex]
    #21369600 - 03/06/15 07:34 AM (9 years, 15 days ago)

Did this a week ago and now I have 12 fully colonized quarts of rye from 3 pf cakes. Just did it as an experiment and it worked great.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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OfflineArtex
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Re: transplanting PF jars to Rye.... [Re: theidiotnoob]
    #21372257 - 03/06/15 07:57 PM (9 years, 15 days ago)

Did you cut from the cakes or use the mycelium covered base of mushrooms (just saw a video where the latter was used to grow oyster mushrooms in coffee grounds)?

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Offlinerandoman
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Re: transplanting PF jars to Rye.... [Re: Artex]
    #21372296 - 03/06/15 08:06 PM (9 years, 15 days ago)

Old thread damn!:wellidunno:


--------------------
The possibility of physical and mental collapse is now very real. No sympathy for the Devil, keep that in mind. Buy the ticket, take the ride. Hunter S. Thompson
The Noob Forum:jah:Getting started:bow2::rockon::rockon:
Wanna Trade?

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OfflineArtex
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Re: transplanting PF jars to Rye.... [Re: randoman]
    #21373592 - 03/07/15 08:09 AM (9 years, 14 days ago)

It was, but I've already gotten useful verification of what I was looking for.

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Offlinerandoman
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Re: transplanting PF jars to Rye.... [Re: Artex]
    #21373609 - 03/07/15 08:15 AM (9 years, 14 days ago)

:thumbup: makes sense to me bud.


--------------------
The possibility of physical and mental collapse is now very real. No sympathy for the Devil, keep that in mind. Buy the ticket, take the ride. Hunter S. Thompson
The Noob Forum:jah:Getting started:bow2::rockon::rockon:
Wanna Trade?

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: randoman]
    #21374949 - 03/07/15 03:17 PM (9 years, 14 days ago)

i put the pf cake in a ziplock bag and mashed it into tiny bits, then transferred into quart jars of rye berries in an sab, just like how you would do a normal g2g. it worked just fine.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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Offlinerandoman
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Re: transplanting PF jars to Rye.... [Re: shroom_sensai]
    #21374964 - 03/07/15 03:22 PM (9 years, 14 days ago)

I use a cheese grater! I have never tried without for a comparison though.


--------------------
The possibility of physical and mental collapse is now very real. No sympathy for the Devil, keep that in mind. Buy the ticket, take the ride. Hunter S. Thompson
The Noob Forum:jah:Getting started:bow2::rockon::rockon:
Wanna Trade?

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: randoman]
    #21374978 - 03/07/15 03:24 PM (9 years, 14 days ago)

hell you could probably do it with a knife on a cutting board and still get good results.

i just tried this out on a lark because i had an old syringe sitting around and hadnt played with pf cakes in forever.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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Offlinerandoman
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Re: transplanting PF jars to Rye.... [Re: shroom_sensai]
    #21374984 - 03/07/15 03:25 PM (9 years, 14 days ago)

The grater is a pain in the ass. I wonder if it is really worth the effort.


--------------------
The possibility of physical and mental collapse is now very real. No sympathy for the Devil, keep that in mind. Buy the ticket, take the ride. Hunter S. Thompson
The Noob Forum:jah:Getting started:bow2::rockon::rockon:
Wanna Trade?

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Offlinerandoman
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Re: transplanting PF jars to Rye.... [Re: randoman]
    #21374994 - 03/07/15 03:26 PM (9 years, 14 days ago)



This is after 2 days though! Coir and verm via bucket tek.


--------------------
The possibility of physical and mental collapse is now very real. No sympathy for the Devil, keep that in mind. Buy the ticket, take the ride. Hunter S. Thompson
The Noob Forum:jah:Getting started:bow2::rockon::rockon:
Wanna Trade?

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: randoman]
    #21375005 - 03/07/15 03:29 PM (9 years, 14 days ago)

yeah, i doubt the grater is worth it.

i also used some of the cakes (12 of them actually) to spawn to cvg in a 27 qt mini mono and ended up harvesting 6 ounces dry, much better than you would ever expect from 12 pf cakes in an sgfc.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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Offlinespacechildo
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Re: transplanting PF jars to Rye.... [Re: shroom_sensai]
    #21375014 - 03/07/15 03:31 PM (9 years, 14 days ago)

Quote:

shroom_sensai said:
i put the pf cake in a ziplock bag and mashed it into tiny bits, then transferred into quart jars of rye berries in an sab, just like how you would do a normal g2g. it worked just fine.




did you use modified lids or did you dispose of the dry verm barrier in the SAB?

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: spacechildo]
    #21375039 - 03/07/15 03:40 PM (9 years, 14 days ago)

actually, i dumped the dry verm barrier, then dropped the cake out of the jar and into a ziploc bag and sealed it. then transferred the bag into my sab. once the cake is colonized its pretty hard to contaminate it.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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Offlinerandoman
KING
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Re: transplanting PF jars to Rye.... [Re: shroom_sensai]
    #21375045 - 03/07/15 03:41 PM (9 years, 14 days ago)

6oz:wellhellothere:


--------------------
The possibility of physical and mental collapse is now very real. No sympathy for the Devil, keep that in mind. Buy the ticket, take the ride. Hunter S. Thompson
The Noob Forum:jah:Getting started:bow2::rockon::rockon:
Wanna Trade?

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: randoman]
    #21375050 - 03/07/15 03:43 PM (9 years, 14 days ago)

no joke man, 6 flushes total, i couldnt believe it. im an oak ridge believer right now.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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Offlinespacechildo
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Re: transplanting PF jars to Rye.... [Re: shroom_sensai]
    #21375474 - 03/07/15 05:21 PM (9 years, 14 days ago)

Quote:

shroom_sensai said:
actually, i dumped the dry verm barrier, then dropped the cake out of the jar and into a ziploc bag and sealed it. then transferred the bag into my sab. once the cake is colonized its pretty hard to contaminate it.




but the cake itself can pick up contams from the air that can germinate on
the fresh and uncolonized grains.

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Offlineshroom_sensai
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Re: transplanting PF jars to Rye.... [Re: spacechildo]
    #21375811 - 03/07/15 07:03 PM (9 years, 14 days ago)

this is true, but i had no problems with it, ultimately its only in the open air for less than a second.


--------------------
My Adventures Cultivating Mescaline Bearing Cacti

“Animals are something invented by plants to move seeds around. An extremely yang solution to a peculiar problem which they faced.”
– Terence McKenna

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