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Offlinecactusjack
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Quart jars...how many cc's per jar?
    #9227047 - 11/11/08 08:53 PM (15 years, 4 months ago)

I plan on innoculating quart-size jars filled with rye...how many ccs should I inject into each jar? Could 1 syringe be used for 10 jars?

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Offlinefungiamongi2
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Re: Quart jars...how many cc's per jar? [Re: cactusjack]
    #9227054 - 11/11/08 08:54 PM (15 years, 4 months ago)

yes perfect.  1 cc per jar.  10 cc syringe.


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Offlinemozartvicious
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Re: Quart jars...how many cc's per jar? [Re: fungiamongi2] * 1
    #9227085 - 11/11/08 09:01 PM (15 years, 4 months ago)

yeah, I concur, 1 cc, inoculate then shake the jar some to disperse spores.
Also, I'm getting ready to do my first G2G (grain to grain) transfers... taking 1/10th of a fully colonized jar and adding it to 10 different sterilized uninoculated substrate jars.
Which may interest you if you're new to grain such as I am.


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Offlinecactusjack
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Re: Quart jars...how many cc's per jar? [Re: mozartvicious]
    #9227127 - 11/11/08 09:10 PM (15 years, 4 months ago)

Thanks everyone. I only have 1 syringe and 12 jars, so a G2G or LC may be what I need for the last few jars.

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Offlinewidowmaker487
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Re: Quart jars...how many cc's per jar? [Re: cactusjack] * 1
    #9227207 - 11/11/08 09:21 PM (15 years, 4 months ago)

From my very limited but good experience,  I will never mess with straight spores ever again.  I think Lc is the best way to go period.  You can knock up and endless supply of lc with only 1cc, use that to knock about endless jars,  plus colonization is about twice as fast.  LC is da shit


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I am what I eat straight up Earth, right.
Nothing but a walking sack of Earth nice to meet you how do ya do.
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OfflineLightShedder
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Re: Quart jars...how many cc's per jar? [Re: widowmaker487]
    #9227252 - 11/11/08 09:30 PM (15 years, 4 months ago)

I use 3 ccs. Also, slowly squirt a stream across the inside of the jar. If that doesn't make sense, you wanna try and squirt the liquid across as much of the sides of the jar as possible. If you can manage, try and get the whole 3 ccs to last you until you have gone all the way around once while inside. You want as much of the circumfrence of the jar as possible to have liquid on it. This way, when the liquid begins to colonize, rather then just starting from one general inoculation point, the mycelium will start to grow from the whole side of the jar making total colonization time faster.


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Offlinenykon
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9227298 - 11/11/08 09:38 PM (15 years, 4 months ago)

I Use 1 CC per jar and do what he said!!^^^^^

also G2G is great, you just have to remember not to do it more than 2 generations down.  you will have to eventually start over.


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OfflineBoris
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9227306 - 11/11/08 09:40 PM (15 years, 4 months ago)

LightShedder, I wouldn't agree with you... Your opinion is kinda a misleading one. First, when innocing, you are also adding water, and you need to be very particular about your moisture content in your substrates... secondly, squiring the fluid around the jar in a certain matter is more of just some OCD manner you posses... why does the way that you introduce the spoors to grain matter, when you shake it all up after innocing any ways ?


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AMU

Terence McKenna Said:
"We have to create culture, don't watch TV, don't read magazines, don't even listen to NPR. Create your own roadshow. The nexus of space and time where you are now is the most immediate sector of your universe, and if you're worrying about Michael Jackson or Bill Clinton or somebody else, then you are disempowered, you're giving it all away to icons, icons which are maintained by an electronic media so that you want to dress like X or have lips like Y. This is shit-brained, this kind of thinking. That is all cultural diversion, and what is real is you and your friends and your associations, your highs, your orgasms, your hopes, your plans, your fears. And we are told 'no', we're unimportant, we're peripheral. 'Get a degree, get a job, get a this, get a that.' And then you're a player, you don't want to even play in that game. You want to reclaim your mind and get it out of the hands of the cultural engineers who want to turn you into a half-baked moron consuming all this trash that's being manufactured out of the bones of a dying world."

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OfflineThe shroomy 1
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9227307 - 11/11/08 09:40 PM (15 years, 4 months ago)

I personally like the idea of keeping the squirt perfectly in line with the holes.  I have 4 inoculation holes and expect to see 4 vertical lines of mycelial growth.  If I see ANY TYPE OF GROWTH outside of those 4 vertical lines I know instantly that I'm dealing with some type of contamination.

See what I mean, 

Also,  3cc's per qt really is a waste of spores.  The above picture was of quart jars with a total of 1cc per jar.

The Shroomy 1


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AMU Q&A thread.

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Invisiblenoobieshroomie
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9227316 - 11/11/08 09:41 PM (15 years, 4 months ago)

yea 1 cc per jar is fine
just make sure you shake the syringe to distribute the spores evenly
after inoculation shake jar to distribute the spores
let sit till 20-30% colonized then shake
let them go to 100% then give them 2-3 days after that


to make a lc you only need 2 or 3 drops theres enough spores there to get a healthy lc
make a lc that way you have more inoculate to do more jars later


  -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineLightShedder
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9228031 - 11/11/08 11:46 PM (15 years, 4 months ago)

I'm not going to say that your way is wrong and I would appreciate it if you would do the same because I assure you that the tip I provided is used successfully by many people. It doesn't throw the water content of any grain off doing what I described because 3 mls of liquid evenly shot around the hole perimeter of the jar is basically unnoticeable. LC is soooooo easy to create an ungodly amount of so why not speed things up by using a little extra? And you ask how my way makes it any faster when you shake??? Well, Ill explain it to you. If you squirt it all in one spot, or even 4 different spots, lets ay that that "spot" of mycelium takes up 2 square inches and is colonized over 100 kernels of XXX grain (just for arguments sake). Well when I spray the LC all around the jar, it will all reform and be shakeable mycelium in the same amount of time as your method but this way, approximately 12 sq inches and 600 kernels of XXX grain will be colonized and evenly distributed in the jar upon shaking. You tell me which one will be colonized quicker.

BTW use a cheap but effective SEALED still air glove box to virtually eliminate contaminations.


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Edited by LightShedder (11/11/08 11:51 PM)

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OfflineLightShedder
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Re: Quart jars...how many cc's per jar? [Re: noobieshroomie]
    #9228044 - 11/11/08 11:48 PM (15 years, 4 months ago)

Hey noob... if inoculating with LC, it is a bad idea to shake immediately after inoculation because it will cause the mycelium in the LC to kinda delay for a few days. I've experimented and found it to be faster overall if you just inoculate, wait for mycelium to form, then shake once. I don't know if this is the same for just spore diluted liquid but as far as live mycelium (in liquid) it kinda slows things down. Now if you are inoculating your qt. jars with agar then it definitely speeds things up to shake immediately after inoculating.

Edit: I thought the OP said it was LC but it looks like he might be talking about spores.


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Edited by LightShedder (11/11/08 11:52 PM)

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Invisiblenoobieshroomie
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9228090 - 11/11/08 11:56 PM (15 years, 4 months ago)

all the jars i have done with LC i just inoculated 1 cc of lc
gave them a shake then wait
usualy 10 days or less the jar is 100% colonized
then i wait 2 or 3 days after before i mix with my sub


  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineLightShedder
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Re: Quart jars...how many cc's per jar? [Re: noobieshroomie]
    #9228246 - 11/12/08 12:39 AM (15 years, 4 months ago)

Hmm that sounds good. Ill try that.


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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9228266 - 11/12/08 12:45 AM (15 years, 4 months ago)

with lc i have QT jars 100% in 10 days or less
inject 1 cc of lc and WAIT let it colonize
then spawn to bulk:thumbup:

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineLightShedder
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Re: Quart jars...how many cc's per jar? [Re: noobieshroomie]
    #9228271 - 11/12/08 12:47 AM (15 years, 4 months ago)

Thats the same time I get but Id much rather not have to go back to them 3 or 4 days later to shake them. Itd be better to just shake them after inoculating for sure. Ill see how it works.


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OfflineThe shroomy 1
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Re: Quart jars...how many cc's per jar? [Re: LightShedder]
    #9228344 - 11/12/08 01:03 AM (15 years, 4 months ago)

Quote:

LightShedder said:
I'm not going to say that your way is wrong and I would appreciate it if you would do the same because I assure you that the tip I provided is used successfully by many people. It doesn't throw the water content of any grain off doing what I described because 3 mls of liquid evenly shot around the hole perimeter of the jar is basically unnoticeable. LC is soooooo easy to create an ungodly amount of so why not speed things up by using a little extra? And you ask how my way makes it any faster when you shake??? Well, Ill explain it to you. If you squirt it all in one spot, or even 4 different spots, lets ay that that "spot" of mycelium takes up 2 square inches and is colonized over 100 kernels of XXX grain (just for arguments sake). Well when I spray the LC all around the jar, it will all reform and be shakeable mycelium in the same amount of time as your method but this way, approximately 12 sq inches and 600 kernels of XXX grain will be colonized and evenly distributed in the jar upon shaking. You tell me which one will be colonized quicker.

BTW use a cheap but effective SEALED still air glove box to virtually eliminate contaminations.




Was that meant for me?


--------------------


AMU Q&A thread.

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OfflineStimpy913
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Re: Quart jars...how many cc's per jar? [Re: The shroomy 1]
    #9228406 - 11/12/08 01:21 AM (15 years, 4 months ago)

i just eat shrooms then poop in big jars. It works if you eat nothing but whole grain for a few weeks.


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OfflineThe shroomy 1
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Re: Quart jars...how many cc's per jar? [Re: Stimpy913]
    #9228420 - 11/12/08 01:24 AM (15 years, 4 months ago)

Quote:

Stimpy913 said:
i just eat shrooms then poop in big jars. It works if you eat nothing but whole grain for a few weeks.




:rofl:  That was hilarious!

The Shroomy 1


--------------------


AMU Q&A thread.

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OfflineLightShedder
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Re: Quart jars...how many cc's per jar? [Re: The shroomy 1]
    #9232264 - 11/12/08 05:28 PM (15 years, 4 months ago)

No it was to Boris.


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