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Invisibledjmako7
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The koh samui experiment
    #9189105 - 11/05/08 01:33 PM (15 years, 4 months ago)

About 4 weeks ago a little idea conjured in my head to experiment a little to learn more about the hardiness and resistance of cubensis.

I had months old straw still a little green but dry enough to burn instantly. I boiled for 3 hours and drained. Then stuck in a pillow case filled with 25% coffee 50% straw. I boiled for 90 minutes and let drain for an hour. I then added 25% verm. I stuck in jars and steamed like BRF. I inoculated 1cc multi spore in half pints. I threw most of the jars out because nothing happened but I kept 4. Well 1 is almost fully colonized with a very fragrant fungi smell mmm delicious.

So know I have the task of figuring out was to do with it. Should I spawn to coir straw and verm? should I just stick in the fc how it is?


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Edited by djmako7 (11/05/08 01:35 PM)

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Invisibledjmako7
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Re: The koh samui experiment [Re: djmako7]
    #9191452 - 11/05/08 08:45 PM (15 years, 4 months ago)

Any idea on how I go about spawning this?


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Offlineveda_sticks
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Re: The koh samui experiment [Re: djmako7]
    #9191484 - 11/05/08 08:48 PM (15 years, 4 months ago)

Theres no point in using trying to sterilised contaminated substrates since the contamination will have eating alot of the food that would have been benificial to mycelium.

I wouldnt do anything with it other than fruit it as a cake on its own. If you must spawn to at a low ratio with a bulk sub mixed in whatever proportions you see fit.,


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Invisibledjmako7
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Re: The koh samui experiment [Re: veda_sticks]
    #9194007 - 11/06/08 08:43 AM (15 years, 4 months ago)

Alright thanks. The mycelium is really strong with weird long root like rhizo the roots spread from one side of the jar to the next and I assume that this is good suggesting it is healthy and strong

here are some pics over time






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Invisibledoze
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Re: The koh samui experiment [Re: djmako7]
    #9194307 - 11/06/08 09:40 AM (15 years, 4 months ago)

yeah i would spawn it to a little bit of bulk. i think it has those long rhizos because its looking for food. i could be wrong though so....


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But I'll bounce back for mine is a heart made of iron forged in the darkest smithy of despair! Alloyed with hate and melancholy! corroded by anger! oxidized by fury!

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Invisibledjmako7
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Re: The koh samui experiment [Re: doze]
    #9236327 - 11/13/08 09:34 AM (15 years, 4 months ago)

The straw cake is doing great, hear have a look




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Edited by djmako7 (11/13/08 09:36 AM)

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Invisibledoze
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Re: The koh samui experiment [Re: djmako7]
    #9236345 - 11/13/08 09:38 AM (15 years, 4 months ago)

cool. what strain?


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But I'll bounce back for mine is a heart made of iron forged in the darkest smithy of despair! Alloyed with hate and melancholy! corroded by anger! oxidized by fury!

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Offlinegrod31
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Re: The koh samui experiment [Re: djmako7]
    #9236358 - 11/13/08 09:41 AM (15 years, 4 months ago)

`dunk and roll!


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Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread

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Invisibledjmako7
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Re: The koh samui experiment [Re: djmako7]
    #9236366 - 11/13/08 09:42 AM (15 years, 4 months ago)

So with this said, This experiment I experienced a 90% failure rate when using straw as a substrate and injecting multispore. It has taken nearly a month and a half for this cake to look like this.
In my honest opinion, if the coffee grounds were not in the jar I do not believe this one would have been a success. As you can see in my previous picture I have some nice rhizome action happening. The center where it spawned was a coffee ground.
I do not expect any fruiting though because of the amount of energy the mycelium took to colonize the straw/verm/coffee mix.

In the future I will do the same thing again but using an LC and more coffee


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Invisibledjmako7
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Re: The koh samui experiment [Re: grod31]
    #9236373 - 11/13/08 09:42 AM (15 years, 4 months ago)

Quote:

grod31 said:
`dunk and roll!




If I dunk it, it will fall apart. I used straw as a substrate and it is quite weak at the bottom. The network connection is not as strong. I did cold shock the cake though


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Edited by djmako7 (11/13/08 09:45 AM)

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Offlinegrod31
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Re: The koh samui experiment [Re: djmako7]
    #9236380 - 11/13/08 09:45 AM (15 years, 4 months ago)

really?.... really that will fall apart? i dunk grain substrates i figure that is less stable then straw. it looks nice and strong.
edit- why in gods name would you have cold shocked?


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread

Edited by grod31 (11/13/08 09:45 AM)

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Invisibledjmako7
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Re: The koh samui experiment [Re: doze]
    #9236387 - 11/13/08 09:45 AM (15 years, 4 months ago)

Quote:

doze said:
cool. what strain?




Strain in the topic title, Koh Samui


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Invisibledoze
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Re: The koh samui experiment [Re: djmako7]
    #9236448 - 11/13/08 09:57 AM (15 years, 4 months ago)

o right. duh.

why not just do the pf tek though?


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But I'll bounce back for mine is a heart made of iron forged in the darkest smithy of despair! Alloyed with hate and melancholy! corroded by anger! oxidized by fury!

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Invisibledjmako7
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Re: The koh samui experiment [Re: grod31]
    #9236463 - 11/13/08 10:01 AM (15 years, 4 months ago)

I cold shocked it to gt it ready to fruit if it does at all. Because it  was in the incubator for so long I figured Id wake up those cells. I placed the mini fruiting chamber outside for one night. It worked quite well, the following 24 hours I noticed more cell expansion than division. Little round white bulbs popped up on the bottom edge of the cake although I do not think it is pins yet.
Like the title says it is an experiment so the procedures it took were and are abnormal.
I do have to say this is a good strain at resisting contams early in the colonization process I did see some motabolites in the jar


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Invisibledjmako7
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Re: The koh samui experiment [Re: doze]
    #9236480 - 11/13/08 10:04 AM (15 years, 4 months ago)

Quote:

doze said:
o right. duh.

why not just do the pf tek though?




I have that going too :smile: although its brazilian im doing for pf tek right now due to the speed of colonization I need atm.

When I grow i like to do a bit of every thing.


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Invisibledoze
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Re: The koh samui experiment [Re: djmako7]
    #9236504 - 11/13/08 10:11 AM (15 years, 4 months ago)

o ok. experimenting is fun.

i like brazilian strain spawned to a little bulk. its amazing what one cake can do when spawned to bulk. :eek:


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But I'll bounce back for mine is a heart made of iron forged in the darkest smithy of despair! Alloyed with hate and melancholy! corroded by anger! oxidized by fury!

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Offlinegrod31
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Re: The koh samui experiment [Re: doze]
    #9236593 - 11/13/08 10:29 AM (15 years, 4 months ago)

cold shocking is obsolete for cubensis. i still think that thing can be dunked. that would be the most beneficial for it.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread

Extras: Filter Print Post Top
Invisibledjmako7
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Re: The koh samui experiment [Re: grod31]
    #9246562 - 11/14/08 09:37 PM (15 years, 4 months ago)

So far I have seen about a half dozen clusters of hyphal knots.


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Invisibledoze
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Re: The koh samui experiment [Re: djmako7]
    #9246570 - 11/14/08 09:38 PM (15 years, 4 months ago)

:thumbup:


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But I'll bounce back for mine is a heart made of iron forged in the darkest smithy of despair! Alloyed with hate and melancholy! corroded by anger! oxidized by fury!

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Offlinedurka_durka420
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Re: The koh samui experiment [Re: doze]
    #9246815 - 11/14/08 10:32 PM (15 years, 4 months ago)

alot of my koh samui 1/4 pints i have ready to be dunked wendsday and thursday have the really long rhizos also and i followed pftek recipie perfect yes i know your doing a diff method but im just comenting on the long rhizos..they are indeed weird look like roots to me.......


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Invisibledjmako7
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Re: The koh samui experiment [Re: durka_durka420]
    #9254349 - 11/16/08 10:59 AM (15 years, 4 months ago)

Quote:

durka_durka420 said:
alot of my koh samui 1/4 pints i have ready to be dunked wendsday and thursday have the really long rhizos also and i followed pftek recipie perfect yes i know your doing a diff method but im just comenting on the long rhizos..they are indeed weird look like roots to me.......




I Kind of like the root like rhizo's it really shows the strains determination to continue cell division

Here are some pins :smile:




The pictures are kind of blurry due to the various depths of what I am taking a picture of.

The bottom pic has a nice pin but its quite blurry and unless you can make it out you wont see it.


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Edited by djmako7 (11/16/08 11:01 AM)

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Invisibledjmako7
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Re: The koh samui experiment [Re: djmako7]
    #9278040 - 11/20/08 11:01 AM (15 years, 4 months ago)

One specimen. More than I expected to come from straw and coffee sub



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Invisibledoze
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Re: The koh samui experiment [Re: djmako7]
    #9278063 - 11/20/08 11:11 AM (15 years, 4 months ago)

its a very pretty one though. are you going to print?


--------------------
But I'll bounce back for mine is a heart made of iron forged in the darkest smithy of despair! Alloyed with hate and melancholy! corroded by anger! oxidized by fury!

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Invisibledjmako7
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Re: The koh samui experiment [Re: doze]
    #9278082 - 11/20/08 11:17 AM (15 years, 4 months ago)

Quote:

doze said:
its a very pretty one though. are you going to print?




Its quite small. I think its due to lack of nutrition. Im just going to pick and dry along with the rest of the aborts. I got another syringe coming of this strain which I like so i'm going to do mono tubs with it then I will print expect giveaways in the future.


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Invisibleslicknick
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Re: The koh samui experiment [Re: djmako7]
    #9278701 - 11/20/08 01:19 PM (15 years, 4 months ago)

give-a-ways are awesome:D
nice experiment by the way


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learning

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Invisibledjmako7
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Re: The koh samui experiment [Re: slicknick]
    #9426585 - 12/13/08 08:23 PM (15 years, 3 months ago)

To conclude this I got 2 mushrooms off of it and they were very potent even for a thai strain. I ate them raw and had a fun morning.

here's the last shot



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