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ch40sth30ry
Stranger
Registered: 10/11/08
Posts: 49
Loc: Rocky Mountains
Last seen: 14 years, 11 months
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Re: 4 days after inoculation no sign of mycelium [Re: J3illy]
#9183427 - 11/04/08 04:16 PM (15 years, 4 months ago) |
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It doesn't get any better. After they colonize, you have to wait another week. After that week, you birth them, then wait another week to two weeks for pins ... then wait for fruits.
It just goes on and on and on ...
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The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 7 months, 3 days
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Re: 4 days after inoculation no sign of mycelium [Re: sixsixsix]
#9187143 - 11/05/08 02:41 AM (15 years, 4 months ago) |
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Quote:
sixsixsix said: I lowered the temp and I guess I'm just going to be patient and give it a week. I just can't stand waiting I just want to see something happen already
Not to be mean my brother, but if you are THAT IMPATIENT, this hobby is going to freakin KILL YOU!!!!
Inoculate your jars.... place in dark place, (room temp), come back and take a peek in about 10 days. By then, you will identify what jars are "Golden", and what jars are contaminated, (If any.).;
The Shroomy 1
-------------------- AMU Q&A thread.
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Spongiform
Some Cow
Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
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Re: 4 days after inoculation no sign of mycelium [Re: The shroomy 1]
#9187453 - 11/05/08 06:49 AM (15 years, 4 months ago) |
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Here's a quote by RR that pretty much sums this up ->
Quote:
And I've been trying to correct that disinformation for years. It's all based on a chart somebody mailed to stamets many years ago showing 86F to be the peak temperature for growth of cubensis on a petri dish, and everybody just accepts it as though Moses carried it down from the mountain on a tablet of stone. However, every single experiment I did to try to duplicate that with extremely accurate temperature monitoring was unable to verify that bogus 86F figure. What I have repeatedly found regardless of strain is that cubensis colonization remains rather flat from about 75F through 81F. Beginning at 83F, the rate of growth falls off sharply. By 86F, growth has slowed down nearly 50% what it was between 75f and 81F. These experiments were conducted on petri dishes that produce little to no heat because of the very thin layer of mycelium. In jars, up to several degrees of heat is produced by the colonizing mycelium; so definitely don't go over 80F to 81F if you're looking for maximum rate of growth. Furthermore, bacteria and thermophilic molds such as Mucor, the black pin mold are stimulated by higher temperatures. Therefore using an incubator set to 86F is certainly favoring bacteria and molds, while slowing down mushroom mycelium growth. Below is a picture of one of my colonization shelves. It sits in my bedroom at normal room temperature and quart jars of rye berries colonize fully in ten days, and pf jars colonize fully in 14 to 21 days, but usually closer to 14. How often do we see posts where people have incubators set at 86F, and they're asking why their jars aren't colonized after four to five weeks, and they have large spots of yellow liquid forming? The liquid is metabolites that the mycelium secretes in response to stress, usually from competitor molds and/or bacteria. What has happened, is they've slowed down the mycelium while stimulating the competitors.
I felt infinitely smarter after reading this collection of RR quotes.
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: 4 days after inoculation no sign of mycelium [Re: Spongiform]
#9187496 - 11/05/08 07:18 AM (15 years, 4 months ago) |
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Quote:
Spongiform said: Here's a quote by RR that pretty much sums this up ->
Quote:
And I've been trying to correct that disinformation for years. It's all based on a chart somebody mailed to stamets many years ago showing 86F to be the peak temperature for growth of cubensis on a petri dish, and everybody just accepts it as though Moses carried it down from the mountain on a tablet of stone. However, every single experiment I did to try to duplicate that with extremely accurate temperature monitoring was unable to verify that bogus 86F figure. What I have repeatedly found regardless of strain is that cubensis colonization remains rather flat from about 75F through 81F. Beginning at 83F, the rate of growth falls off sharply. By 86F, growth has slowed down nearly 50% what it was between 75f and 81F. These experiments were conducted on petri dishes that produce little to no heat because of the very thin layer of mycelium. In jars, up to several degrees of heat is produced by the colonizing mycelium; so definitely don't go over 80F to 81F if you're looking for maximum rate of growth. Furthermore, bacteria and thermophilic molds such as Mucor, the black pin mold are stimulated by higher temperatures. Therefore using an incubator set to 86F is certainly favoring bacteria and molds, while slowing down mushroom mycelium growth. Below is a picture of one of my colonization shelves. It sits in my bedroom at normal room temperature and quart jars of rye berries colonize fully in ten days, and pf jars colonize fully in 14 to 21 days, but usually closer to 14. How often do we see posts where people have incubators set at 86F, and they're asking why their jars aren't colonized after four to five weeks, and they have large spots of yellow liquid forming? The liquid is metabolites that the mycelium secretes in response to stress, usually from competitor molds and/or bacteria. What has happened, is they've slowed down the mycelium while stimulating the competitors.
I felt infinitely smarter after reading this collection of RR quotes.
good read that thread A
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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LoveOverAll
HigherSpiritEvolving
Registered: 07/02/08
Posts: 1,837
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Re: 4 days after inoculation no sign of mycelium [Re: veda_sticks]
#9187608 - 11/05/08 08:13 AM (15 years, 4 months ago) |
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i dont know why i keep clicking on these question, i know the outcome is always the same. there should be a mandatory faq and sticky reading before noobs are allowed to post questions*
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sixsixsix
New
Registered: 09/29/08
Posts: 15
Last seen: 14 years, 3 months
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Re: 4 days after inoculation no sign of mycelium [Re: Spongiform]
#9187676 - 11/05/08 08:41 AM (15 years, 4 months ago) |
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I meant I just want to know that I'm doing it right, I grew hydroponics pot before this and that takes about 3 months but every day you can see change. I just can't stand thinking something is not going right or something was done wrong
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nenl
Strangest
Registered: 07/16/08
Posts: 683
Last seen: 12 years, 4 months
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Re: 4 days after inoculation no sign of mycelium [Re: sixsixsix]
#9187686 - 11/05/08 08:46 AM (15 years, 4 months ago) |
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It's a hard lesson to learn for some of us - myself included. However, once things get rolling, it seems to move almost too fast for me. If you're in this hobby for the long run, I'd recommend starting the process, whether pf tek or grains, each week and you'll almost have constant change from day to day and plenty to keep you busy with nocing, fruiting, harvesting, printing. Then if you get into agar, that adds another dimension to the matrix and can easily take over all your free time if you let it.
-------------------- hehe
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: 4 days after inoculation no sign of mycelium [Re: sixsixsix]
#9187690 - 11/05/08 08:48 AM (15 years, 4 months ago) |
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pot and shrooms are worlds apart like The shroomy 1 said put them away for 10 days or so go on with your normal daily routine then check them what ever you do take our advice or not good luck and keep us posted
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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sixsixsix
New
Registered: 09/29/08
Posts: 15
Last seen: 14 years, 3 months
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Re: 4 days after inoculation no sign of mycelium [Re: noobieshroomie]
#9187704 - 11/05/08 08:51 AM (15 years, 4 months ago) |
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Thats why I found this forum for help, books and everything else does compare to people that have already had success
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