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OfflineIcAnFeeLthErain
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Registered: 05/24/08
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Rhizomorphicness
    #9151790 - 10/28/08 11:24 PM (15 years, 3 months ago)

What's the big deal about Rhizomorphic growth?  I tend to get plenty of rhizo mycelium in BRF jars, but my grain jars are always just puff clouds.  Grain jars colonize fast w/ LC (usually 8-10 days) but is there a certain benefit to rhizomorphic growth that I am unaware of?


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OfflineBrainChemistry
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Re: Rhizomorphicness [Re: IcAnFeeLthErain]
    #9152090 - 10/29/08 12:25 AM (15 years, 3 months ago)

It looks freakin sweet.


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Offlinephaseflux
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Re: Rhizomorphicness [Re: BrainChemistry]
    #9152882 - 10/29/08 05:25 AM (15 years, 3 months ago)

In my experience strong rhizomorphic growth leads to faster colonization. When i started making my BRF jars with less water, i noticed a lot of rhizomorphic growth, while the other jars that i did simultaneously with more water, had more cloudy looking growth. The rhizomorphic jars colonized much faster. I think its because those strands are shooting through the mix and expanding outwards from the strands, covering more area faster.
If you want to see more rhizomorphic growth in your jars just make the jars a bit on the dryer side.

I don't know about grain jars though.


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Offlinedothedew69
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Re: Rhizomorphicness [Re: phaseflux]
    #9152908 - 10/29/08 05:50 AM (15 years, 3 months ago)

Or you can isolate on Agar, but I am under the impression that rhizo growth is faster growth. I got lucky and one of my grain jars just looked amazing with all the rhizomorphic growth and colonized very quickly.


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Offlineveda_sticks
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Re: Rhizomorphicness [Re: phaseflux]
    #9152933 - 10/29/08 06:00 AM (15 years, 3 months ago)

Quote:

Spores from the same print are the norm. There's plenty of genetic diversity within a sporeprint. Rhizomorphic mycelium is strain, therefore genetic related. When isolating strains, isolate rhizomorphic sectors. Not all rhizomorphic sectors will be good producers, but nearly all cottony sectors will be poor producers.

Yes, rhizomorphic mycelium is a good sign that the hyphae have mated. Monokaryons won't configure into rhizomorphs.
RR

Actually, a drier substrate will result in more rhizomorphic growth, as well as faster growth.  Your observation was correct.
RR

ve made numerous transfers of cottony mycelium and it stayed cottony. I've made numerous transfers of rhizomorphic mycelium and it stayed rhizomorphic. I believe there's more involved than we currently know. I have many strains that I've isolated down to single sector rhizomorphs, and these remain rhizomorphic even years later when removed from the refrigerator and transferred to new substrates. I think a lot of confusion comes from ceasing to make transfers before getting down to single sector isolates, thus the process of anastomosis is still occurring which would explain the mycelium changing to different types.

I've found a difference in appearance based on the nutritive substance in the growing medium, with the strands much closer together when highly nutritive media was used, and the strands spread out more when lesser nutritive media was used, but they're still rhizomorphs when viewed under the microscope, even if they don't look so to the unaided eye.

I've attempted to fruit completely cottony isolates and the results were also the same every time. Either they didn't fruit at all, or fruited poorly. Rhizomorphic isolates have almost always produced nice fruitings with only one or two exceptions out of hundreds. I go through about 500 petri dishes per month, so believe me when I say I've repeated these experiments many times over.
RR






the search engine is great

I searched rhyzormorphic and username rogerrabbit, the search engine suggested rhizomorphic (correct spelling) and got a ton of results.


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Offlineairborne robot
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Registered: 10/07/08
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Re: Rhizomorphicness [Re: veda_sticks]
    #9153170 - 10/29/08 08:05 AM (15 years, 3 months ago)

Of the 6 WBS jars I knocked up with OI on my first go I had 1 stall out :frown: 4 grow with a mix of the puffy and branchy white stuff. After shaking the jars at about 40% the puffy jars took longer to get set back up and recover, but the nice stringy rizo growth jar after shaking, exploded with growth in about 10 hours after shaking. This is going to be my set aside jar to make my clones out of and get my future OI batches.
Hopefully the clones come along nice and I can be giving out some prints of the 2nd or 3rd gen OI isolated batches to whoever wants them.


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