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OfflineQuitDrinking
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Registered: 09/04/02
Posts: 52
Loc: SC
Last seen: 21 years, 4 months
Myco Liquid Innoculation, does this sound right?
    #910572 - 09/26/02 07:13 PM (21 years, 8 months ago)

I had some good looking dextrose liquid (+2cc of spore syringe)iin a jar for about 10 days (at room temp) then shook like hell and added 2cc of h2o2 for about a half pint and innoculated pf style jars with about 2-3 cc per jar. Have not seen any sign of growth yet (48 hours later, heard it would be fast) but I shot the main load right down the middle, with a little squirt on the side for visual effects. Never used this method before (have only used spore syringe) am I being impatient? How long before I should see anything or did I screw up the liquid tek?


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Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.

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InvisibleAlkaloids
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Re: Myco Liquid Innoculation, does this sound righ [Re: QuitDrinking]
    #910744 - 09/26/02 08:25 PM (21 years, 8 months ago)

how much innoculant did you add the H2O2 too? That sounds like it might be a bit strong. Vatoloco is right about the adjustment, but my concern would be if you added enough to retard the growth severely.
good luck


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OfflineQuitDrinking
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Re: Myco Liquid Innoculation, does this sound righ [Re: Alkaloids]
    #911632 - 09/27/02 05:05 AM (21 years, 8 months ago)

I went by the club 99 honey water tek which says add 2cc per 1/2 pint . I use the el cheapo 3% h202. Anybody had any experience with this?


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Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.

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OfflineAgarico
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Registered: 09/23/02
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Re: Myco Liquid Innoculation, does this sound righ [Re: QuitDrinking]
    #911664 - 09/27/02 05:57 AM (21 years, 8 months ago)

I'm trying to do the same, I trust CluB99 completely.
I'm sure it will work. :grin:

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OfflineDERRAYLD
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Re: Myco Liquid Innoculation, does this sound righ [Re: Agarico]
    #911673 - 09/27/02 06:07 AM (21 years, 8 months ago)

it works
but really this has been covered and over-covered in this forum.
Just page down , or look 4 posts down there`s great refernce to this exact topic.
I personally don`t see the value of the h2o2.
I`ve made jars of liquid solution that have lasted for ages and ages.
I open the lid leave the liquid exposed to the air while I suck up some solution, I haven`t had contamination problems with my mycelium solution yet.
and it`s been 6 months since I had to make more.

Edited by DERRAYLD (09/27/02 06:10 AM)

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OfflineAgarico
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Re: Myco Liquid Innoculation, does this sound righ [Re: DERRAYLD]
    #911718 - 09/27/02 06:52 AM (21 years, 8 months ago)

Did you inoculate it with a spore solution? I know this tek could also be used to clone a mushroom, I don' t think you can avoid h2o2 then :confused: 

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OfflineQuitDrinking
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Re: Myco Liquid Innoculation, does this sound righ [Re: Agarico]
    #912069 - 09/27/02 11:01 AM (21 years, 8 months ago)

Yes, 2cc of PF in a 1/2 Pint jar of h2o and 1 tsp. dextrose. It grew fine and I agitated everyday. After 10-11 days I added 2cc of 3% h2o2 and innoculated jars. I figured h2o2 would help avoid contams in the transfer process.
Sorry if I'm just being impatient.
I saved a few cc's(of the myco liquid) in the syringes and I'll try to make a new jar and see if their viable still. I can see the wispy white stuff floating all around and nothing unusual looking....


--------------------
Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.

Edited by QuitDrinking (09/27/02 11:17 AM)

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OfflineAgarico
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Re: Myco Liquid Innoculation, does this sound righ [Re: QuitDrinking]
    #913396 - 09/27/02 08:38 PM (21 years, 8 months ago)

I'll tell you a story:
I inoculated 3 PF jars with a spore solution, afrer three weeks I didn't see any germination so i thought the syringe was'n viable anymore. In need of an empty syringe I spit all the syringe in an honey/water solution without sterilize anything and put this in a nasty case in the kitchen with no hope; after a week l saw some spores had germinated so l put a little h2o2 in the jar.
As now it seems healthy mycelium is growing, no sign of contam, l'll keep you updated on this :wink: 

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OfflineAgarico
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Re: Myco Liquid Innoculation, does this sound righ [Re: Agarico]
    #913404 - 09/27/02 08:41 PM (21 years, 8 months ago)

If it works, it's genious :grin: it's a strain l'm missing......

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OfflineDERRAYLD
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Re: Myco Liquid Innoculation, does this sound righ [Re: Agarico]
    #913977 - 09/28/02 12:26 AM (21 years, 8 months ago)

Well when dealing with spores h202 might be detrimental to the developing mycelium.
But cloning is a different story.
That is allready estalbished mycelium which can deal with h202

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OfflineQuitDrinking
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Registered: 09/04/02
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Loc: SC
Last seen: 21 years, 4 months
Eureka !!!! [Re: QuitDrinking]
    #914406 - 09/28/02 07:11 AM (21 years, 8 months ago)

I just went and checked and I found a little fuzzy white growth in 2 of my jars (2 out of 26). I also put about 3 cc of the myc-liquid in a dextrose water jar as I said I would. Is refrigeration an important part of making that stuff eeven if you use it in a couple weeks? cause I didn't refrigerate at all. I'm having a beer to celebrate my success!
Man I gotta quit drinking....


--------------------
Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.

Extras: Filter Print Post Top
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