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Invisiblearp180
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idea for glove box? is it original *DELETED*
    #9118093 - 10/22/08 08:43 PM (15 years, 5 months ago)

Post deleted by arp180

Reason for deletion: Some things are better left in the past



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OfflineCapnSkoo
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Re: idea for glove box? is it original [Re: arp180]
    #9118125 - 10/22/08 08:48 PM (15 years, 5 months ago)

The only one that springs to mind is that the operating principle behind a glove box is still air. Not to bash you. What you have there is something completely different.


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Invisiblemusher_420
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Re: idea for glove box? is it original [Re: arp180]
    #9118132 - 10/22/08 08:49 PM (15 years, 5 months ago)

There is no reason to inoculate inside of a glove box let alone worry about contamination while putting your jars into an incubator.

Also, that filter setup wont do much good.  I've tried it before.  Still air works better.


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OfflineRogerRabbitM
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Re: idea for glove box? is it original [Re: musher_420]
    #9118153 - 10/22/08 08:53 PM (15 years, 5 months ago)

Quote:

What you have there is something completely different.




Hardly.

These have been being made for many years and they actually raise the contamination rates.  Every grower who makes one, takes the fan off after a few uses.  You want STILL air.  Those cheap paper filters don't work or we wouldn't spend hundreds on 5 to 11 inch thick laminar flow filters.  In addition, even a proper filter would raise the contamination rate in a closed box by swirling the air around.  Take the fan and filter off and close the box up when you work, and you'll have great results.
RR


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InvisibleJ3illy
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Re: idea for glove box? is it original [Re: musher_420]
    #9118157 - 10/22/08 08:53 PM (15 years, 5 months ago)

Yea I don't understand the concept of the fan - with the filter on the outside it appears you're sucking air into it? why? i would think blowing air OUT of it would be a better idea - but then what would the point of the filter be?

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Invisiblearp180
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Re: idea for glove box? is it original [Re: musher_420]
    #9118163 - 10/22/08 08:55 PM (15 years, 5 months ago)

does the filter increase chance of contam, or just make working conditions harder? Because i can turn it off, the glove box is air tight besides the HEPA,
The top plexiglass is sealed with a releasable adhesive tape, so i can take it off and put it back on whenever i want and it seals instantly.

I just thought by putting the incubator below and make things easy and mostly was curious if it was an original idea


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
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Invisiblearp180
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Re: idea for glove box? is it original [Re: arp180]
    #9118235 - 10/22/08 09:07 PM (15 years, 5 months ago)

i thought  the fan created positive pressure, and the filter is a real HEPA filter.  It's about two inches thick and 5x4 wide,  It's not a hepa type, it was $40, i thought that was expensive for such a small one.


But that is why i post, to learn.  The air current that comes through the filter is traveling in the same direction as if the fan were behind it.


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


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Invisiblearp180
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Re: idea for glove box? is it original [Re: arp180]
    #9118275 - 10/22/08 09:13 PM (15 years, 5 months ago)

If the fans don't work, why the hundreds of posts on here about positive pressure glove boxes? in no way am i arguing just curious.


When I inoculated everything the jars lids were constructed as follows;

three holes, two with high temp RTV sealant and one with polyfil for GE, then a sheet of tvyek over top.  this is my first attempt so i've got alot to learn.  Because the fan was running during the inoc. do you think ill be ok.  I pc'd the grains for 120min, i did the pf tek jars for 90, and the lc for 20.


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


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OfflineCapnSkoo
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Re: idea for glove box? is it original [Re: arp180]
    #9118301 - 10/22/08 09:16 PM (15 years, 5 months ago)

Time will tell won't it. You also see hundreds of posts about using cakes to get high. Doesn't mean its the best way to trip does it?

EDIT: eating is the wrong word, using is better


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

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Edited by CapnSkoo (10/22/08 09:19 PM)

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OfflineVermonster420
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Re: idea for glove box? is it original [Re: CapnSkoo]
    #9118368 - 10/22/08 09:29 PM (15 years, 5 months ago)

obviously a man who doesn't live by the K.I.S.S. credo...i got a buddy like that...I hang out with him for his ADDERALL lol, it's barely worth it.

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InvisibleJ3illy
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Re: idea for glove box? is it original [Re: CapnSkoo]
    #9118373 - 10/22/08 09:30 PM (15 years, 5 months ago)

I would think a fan w/ an expensive filter like that would be better suited for a fruiting chamber - to perform FAE.. If you sterilize your glove box nice and fresh before you use it - having zero air movement in there is what you want.. Why would you ever want air being sucked into it? It doesn't make any sense.. Instead of having it sealed off and knowing it's contaminant free - you're bringing air in, and even tho it's filtered, you're still running the risk of a contam getting in..

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Invisiblearp180
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Re: idea for glove box? is it original [Re: CapnSkoo]
    #9118433 - 10/22/08 09:39 PM (15 years, 5 months ago)

okay took your advice, just inoculated an lc and turned the fan off for a still air environment.  however i did catch my plastic glove on fire due to the alcohol when i flamed my needle.  now ive got a nice burnt spot on my carpet.  Better that than my hand.  Lesson learned


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


Extras: Filter Print Post Top
OfflineCapnSkoo
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Re: idea for glove box? is it original [Re: arp180]
    #9118502 - 10/22/08 09:49 PM (15 years, 5 months ago)

:rofl2:

I wonder how many innoc related fires occur every month?

From GB explosions to flaming gloves.


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

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Invisiblenoobieshroomie
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Re: idea for glove box? is it original [Re: CapnSkoo]
    #9118523 - 10/22/08 09:52 PM (15 years, 5 months ago)

:onfire:

  -noobie-


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not sure what GE and FAE are but i should probably get some.

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OfflineRogerRabbitM
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Re: idea for glove box? is it original [Re: noobieshroomie]
    #9118599 - 10/22/08 10:04 PM (15 years, 5 months ago)

You only need to use soap and water to clean a still air box.  In fact, you'll be better off taking the gloves out and cutting holes just the right size for your arms to go through with a snug fit.  This way,  you can pull the syringe or scalpel out to flame sterilize, and then put it back in while still red hot.

I didn't mean to appear to be bashing earlier.  It's just that so many growers have made 'positive pressure' boxes, and then experience a worse contamination rate than working in open air, because of all the turbulence created.  You want still air for best results.
RR


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Invisiblearp180
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Re: idea for glove box? is it original [Re: RogerRabbit]
    #9118699 - 10/22/08 10:18 PM (15 years, 5 months ago)

no hard feelings here RR, i just want to learn. I wish I would of found this out yesterday.  i did 2 3lb rye bags, 12 1/2 pint jars(6 pf tek, 6 hard red winter wheat berries), 3 pint jars of wheat berries and two lc's.  All in the glove box, but the second lc i had the fan off via your advice.  Besides the fan I used extremely sterile teks, went way over board actually.  Do you think the fans going to screw me RR, or should i still get at least a few to colonize and play with. Like I said earlier i spent a good deal on the HEPA filter, its no flimsy paper thing, and the box is all siliconed up including all around the filter and fan. So if contams did get in, they came through the filter most likely.


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


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OfflineRRVideoV
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Re: idea for glove box? is it original [Re: arp180]
    #9118778 - 10/22/08 10:29 PM (15 years, 5 months ago)

I doubt seriously the computer fan could draw air through the filter anyway.  The problem is the fan swirling around the air and contaminants that are already in the box when you seal it up.  Cross your fingers and hope for the best.

Another tip for still air boxes is to mist the walls, ceiling and floor with plain water before going to work.  The water will trap and hold contaminants while you do your sterile work.  Remember, neither the box, nor the air inside needs to be sterile.  Still air is what you want.  This becomes more and more important when you do agar and petri dish work, where you lift the lids on petri dishes and grain jars to make inoculations with agar wedges.  If you were working with syringes and you flamed the needles red hot, you should be fine.
RR


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Invisiblearp180
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Re: idea for glove box? is it original [Re: RRVideo]
    #9119004 - 10/22/08 11:06 PM (15 years, 5 months ago)

thanks for the info, it all makes sense now.  there is just so much contradictory information out there.  to each his own


--------------------
"Given the choice between the experience of pain and nothing, I would choose pain."  William Faulkner

"That which exists without my knowledge exists without my consent."
-A quote from the Judge in the novel Blood Meridian; or the Evening Redness in the West by Cormac McCarthy

"Let there be light" My Quick Reference Guide to Lighting
My AutoMono (11oz First Flush)
My Monster Mushroom Mono (9.3oz First Flush)


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Invisibletripchip
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Re: idea for glove box? is it original [Re: arp180]
    #9119317 - 10/23/08 12:27 AM (15 years, 5 months ago)

Quote:

arp180 said:
If the fans don't work, why the hundreds of posts on here about positive pressure glove boxes? in no way am i arguing just curious. 




ok then why did you ask twice whether or not your idea was original???  you obviously knew it wasnt.

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OfflineHippieChick
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Re: idea for glove box? is it original [Re: arp180]
    #9120378 - 10/23/08 09:37 AM (15 years, 5 months ago)

Quote:

arp180 said:

I just thought by putting the incubator below and make things easy and mostly was curious if it was an original idea




Nope. Not original



I've never had contam issues using mine with the fan on , although I do use it just as often with the fan off . All depends on what I'm doing .

If I'm going to be loading large quantities of jars through the side hole I leave it on , that way I'm not pushing air into the glovebox while I'm loading the jars:thumbup:

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