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Its a rash, scratch it.

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"After the people are dead, after the things are broken and scattered, taste and smell alone, more fragile but more enduring, more unsubstanial, more persistent, more faithful, remain poised a long time, like souls, remembering, waiting, hoping, amid the ruins of all the rest."
-Marcel Proust

[This message has been edited by Sclorch (edited December 15, 1999).]

My question was not "is 5-HTM the proper designation for serotonin?"

My question was, "Has anyone had success with 5-hydroxytryptamine (serotonin) added to the substrate?"

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Its a rash, scratch it.

Ayahuasca Substrate

I provided many links to sources for many different possible substrate additives. There have been many different posts made on this topic in the past so a search may be good. Has anyone thought of what might be the product of this additive? 5-HO-DMT (Bufotenine)? 4,5-DiHO-DMT? Or just 4-HO-DMT (Psilocin)? Check out the link above for more info.

-Youjutsu

"In Psilocybe cubensis mushrooms, approximately 22% of labeled tryptamine was incorporated into psilocybin. The addition of tryptamine hydrochloride to substrate increases yield of psilocybin in dried mushrooms from 0.01-0.2% to 3.3%. (Gartz 1989) (Stamets 1996).
A concentration of 25 millimoles of tryptamine hydrochloride is added to 10 grams mushroom substrate (spawn media). The substrate is composed of either rye grain spawn or rice spawn. The following spawns can be used for the culture of many species of mushrooms:
Rye Grain Spawn: 50 grams of rye grain
65 mL of water
Cow manure/Rice spawn:
0.5 cup of dried cow manure
0.25 cup of rice grain
1.5 cups of water
The spawn is placed in one pint wide mouth canning jars and pressure cooked for 30 minutes. A culture grown on PDA or MEA media is inoculated into the spawn jars and allowed to grow until the jar is completely covered in mycelium. A large aquarium can be used to cultivate the mushrooms for identification."

The rest of the entry just goes on to describe how to fruit; casing, etc.

Anyway, the reference to Gatrz is:

Gartz, J.; Biotransformation of Tryptamine in Fruiting Mycelia of Psilocybe cubensis; Planta Medica (1989) 55: 249-250

For anyone that's really into finding out, this may be a good place to look.

[This message has been edited by TriGuy (edited December 18, 1999).]

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Peace and Love, Baby!

-PsiliPharm

[This message has been edited by PsiliPharm (edited December 18, 1999).]

As for the 25 millimoles of tryptamine, I did a quick calc and came up with 4.00 grams. This agrees with what everyone else has been saying. (Used C10H12N2 and a mol. wt. of 160.22 for tryptamine)

I think what Uriah is referring yo with the drying of tryptamine enhanced shrooms is that psilocin is far more sensitive to light, air and heat than psilocybin, so just drying them would defeat the purpose of growing with tryptamine in the first place (assuming that psilocin is mainly the product from this).

If you're using typtamine hydrochloride, the molecular weight will actually be 196.68, so 25 millimoles of TryHCl would be 4.92 grams.

-PsiliPharm