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Offlinexswaveyx
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Easiest way to knock up bulk quart jars
    #9101762 - 10/19/08 08:43 PM (15 years, 4 months ago)

Well, everything is in place except for my spores. im knocking up 80-100 quart jars, im guessing closer to 100 since we have them.... but im going straight from spores. I have tons of PESA prints that would easily do the job, but the plan was to have 20 of each strain (4 straings), and then make some extras. So pretty much i need to make 25 quarts out of each strain. What would be the easiest approach? Buy a print, and liquify it down.... buy 8 syringes? How much do i really need to get this going?I know it would be easier to knock up a jar of rye and let it colonize, then spread to other jars.... but thats not what im trying to do, will be doing 14 jars a day and trying to do it all as close together as we can. Thanks


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars *DELETED* [Re: xswaveyx]
    #9101840 - 10/19/08 09:06 PM (15 years, 4 months ago)

Post deleted by flameclown

Reason for deletion: [this post is damn old]


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OfflineBuckeye Oysters
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Re: Easiest way to knock up bulk quart jars [Re: flameclown]
    #9101847 - 10/19/08 09:09 PM (15 years, 4 months ago)

1 print will make 6+ syringes.  1 syringe will shoot up 20 jars if 1/2cc injection is used, so just one print and 5 syringes would be sufficient to do 100 jars.


--------------------
Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101850 - 10/19/08 09:10 PM (15 years, 4 months ago)

Well if you have a tsc or a rule king. Its a farm supply place you can get all the luker syringes you need. If you have sterile prints i would do LC. Most will steer you away from multi spore LC but Ive had pretty good results, I do like to isolate before LC but if your in a hurry thats the way to go for your bulk that you seem to want. If your doing 100 quart jars and say they are a success you have a lot of work ahead of you my friend.:cheers:


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Offlinegianimon
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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101880 - 10/19/08 09:19 PM (15 years, 4 months ago)

Quote:

will be doing 14 jars a day and trying to do it all as close together as we can




Quote:

I know it would be easier to knock up a jar of rye and let it colonize, then spread to other jars.... but thats not what im trying to do




but that's the quickest method to achive your goals.  overall times is much better when you can do everything at once like inoc all jars at once.  buy the 4 strains you want in syringes unless your sterile procedures are really good for making a syringe from print.  four syringes are cheaper than 4 prints :wink:

i do about 70-80 jars at a time.  this is how you should do it IMO.  or by spawn bags, my next purchase :wink:

1. sterilize a first round of 8, 1/2 pint jars.  only put a little WBS, etc in each jar, 1/8-1/4 full.  we use a little spawn so it will colonize faster to 100%.

2. knock up your 8 jars with like 1/2cc each.  2 jars per strain incase of contamination. 

3. sterlize all your remaining jars so you have 100 sterile jars waiting for you.  a sterilized jar can last a long time b4 your inoculate.  ive let mine go a few weeks and the moisure content is fine.  just make sure you don't have too large air holes, i use 1/4" but most people use 1/16-1/8". 

4. once the jars you knocked up are 90-100% colonized and free of contaim sterilize 4 quart jars 1/2 full with distilled water.

5. break up spawn in jar to loosen WBS, rye,etc.

6. take a sterile syringe and take some sterile water out of a jar and inject it into a colonized jar. shake jar to get myc into the water.

7. use syringe to take out myc laden water.  i like to take out like 30cc of myc water.

8. inject myc water into jar of sterile water.

9. you now have a real deal LC which is practically guaranteed to be contam free if your sterile.  the LC can be used immediately and the LC should be ready by the time your done PCing 100 jars...trust me PCig that many jars takes A LONG TIME!!! (including a 23 qt PC, heat up, cool down and sleeping somewhere in between).

10. use syringe to take some of the LC and inoculate all your 100 jars as you wish with each strain.


--------------------
life is short - drink it up!~~~!

Edited by gianimon (10/19/08 09:22 PM)

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101893 - 10/19/08 09:21 PM (15 years, 4 months ago)

why the fuck would you make 80 quart jars of grain?

First, if you're set on using grain, try using autoclavable bags.  You can make 1 bag of substrate that is up to 4 quarts.  You only need 1 inoculation point.  You can easily mix the grain up, so colonization is much faster.

Second, Make 8 quarts of grain, and learn how to use hpoo.  Bulk production is not done using grain.

Third, grains produce small shrooms.  Hpoo produces monsters.  8 quarts of grain spawned to the proper amount of Hpoo could easily produce 4-8lbs of dried cubensis.  And you dont have to run the pressure cooker only once.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.

Edited by derx (10/19/08 09:28 PM)

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Offlinexswaveyx
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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9101909 - 10/19/08 09:26 PM (15 years, 4 months ago)

Quote:

gianimon said:
Quote:

will be doing 14 jars a day and trying to do it all as close together as we can




Quote:

I know it would be easier to knock up a jar of rye and let it colonize, then spread to other jars.... but thats not what im trying to do




but that's the quickest method to achive your goals.  overall times is much better when you can do everything at once like inoc all jars at once.  buy the 4 strains you want in syringes unless your sterile procedures are really good for making a syringe from print.  four syringes are cheaper than 4 prints :wink:

i do about 70-80 jars at a time.  this is how you should do it IMO.  or by spawn bags, my next purchase :wink:

1. sterilize a first round of 8, 1/2 pint jars.  only put a little WBS, etc in each jar, 1/8-1/4 full.  we use a little spawn so it will colonize faster to 100%.

2. knock up your 8 jars with like 1/2cc each.  2 jars per strain incase of contamination. 

3. sterlize all your remaining jars so you have 100 sterile jars waiting for you.  a sterilized jar can last a long time b4 your inoculate.  ive let mine go a few weeks and the moisure content is fine.  just make sure you don't have too large air holes, i use 1/4" but most people use 1/16-1/8". 

4. once the jars you knocked up are 90-100% colonized and free of contaim sterilize 4 quart jars 1/2 full with distilled water.

5. break up spawn in jar to loosen WBS, rye,etc.

6. take a sterile syringe and take some sterile water out of a jar and inject it into a colonized jar. shake jar to get myc into the water.

7. use syringe to take out myc laden water.  i like to take out like 30cc of myc water.

8. inject myc water into jar of sterile water.

9. you now have a real deal LC which is practically guaranteed to be contam free if your sterile.  the LC can be used immediately and the LC should be ready by the time your done PCing 100 jars...trust me PCig that many jars takes A LONG TIME!!! (including a 23 qt PC, heat up, cool down and sleeping somewhere in between).

10. use syringe to take some of the LC and inoculate all your 100 jars as you wish with each strain.




You only use 1/2cc on a quart jar? Geeze... ive been using 1cc on my jelly jars... 1/4 in each hole. So then i should put one hole directly in the middle, and inject there?

Let me get this straight

Knock up 8 quarts, 2 of each spawn
Let fully colonize
break it up, spray in 30 cc water, shake up, draw back again
Knock up all other jars with the water aquired

right?

The way i see it, i have 2 16 qt pc's... 7 fit in one...so that 14 i can do at a time, thats a week worth of Pc'ing everyday... not really that bad, especially if i just store them all in bins and knock them up all on the same day. the most ive ever let one sit without inoculation was 3 days and i swore it wasnt gonna work, i was really hesitant about it.

Do i have it right? Then ill just have 2 of each jar to play with for the time being i guess huh? What if i dump all 8 of those jars (4 diff strains) into one monotub and mix it up? would i get a bunch of diff shroomies poppin up everywhere? that would be pretty neat!


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Offlinegianimon
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101913 - 10/19/08 09:28 PM (15 years, 4 months ago)

i make 80+ quart jars of spawn because when i bought them a long time ago i didnt know about bags at that time and jars last for ever and id rather loose a few jars to contam then a few bags :wink:  i dont like waste and a big plastic bag is a big waste when i cant be reused very well.

i think the OP is only talking about inoculation of spawn which is rye.  who knows what the OP will spawn into.  if the OP has 100 jars i sure hope he/she knows about hpoo and other bulk subs.


--------------------
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Offlinexswaveyx
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101916 - 10/19/08 09:29 PM (15 years, 4 months ago)

Well, i have a 10lb bag of rye sitting here...i was pretty set on using it. Im going to be using coir/verm for casing...not hpoo. Inform me on the bags a little more? I saw them but didnt really understand how to do it, so i thought id just stick with what i know. I havent bought any jars yet, so now is the time to decide.... although itll only cost me 20 bucks for over 100 quart jars from my neighbor... less time sounds better.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9101931 - 10/19/08 09:35 PM (15 years, 4 months ago)

It costs more up front for the bags and the impulse sealer but they are MUCH easier to use, especially when you're trying to do bulk production.  $20 for 100 jars sounds like a steal. 

If you inoculate each jar at 4 different sites, that leads to 4 variables for error each quart.  multiplied by 80 quarts is 320 chances to make an error. 

Now, if you made 2 quart bags and inoculated each bag one time with 1ml of spore solution, you leave only 40 chances for error to make the same amount of substrate.

Shaking up half colonized bags is much easier than shaking up a half colonized jar.



PS.  you can still use rye.  I said grains and that includes rye, WBS, corn, whatever you want.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.

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Offlinexswaveyx
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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101933 - 10/19/08 09:36 PM (15 years, 4 months ago)

so then you use a bag for 4 quarts of rye. And if i have a 16 quart pc, i could probly fit 3 in there at once right? Double that... thats 24 quarts a night. Then i could use 1 syringe of each strain to knock them up easy? Right?...  Unless they dont hold as much as i thought?


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

Extras: Filter Print Post Top
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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101940 - 10/19/08 09:38 PM (15 years, 4 months ago)

I wouldnt reccomend making 4 quart bags until you have your method down precisely.  Start small and then scale up.  Make a few 1quart bags and if that is successful, scale up to 2, then 3... etc...

The chances for wet spot developing increases ten-fold when having 4quarts of wet ryeberries sitting in 1 bag.  Thats why it's key to not overhydrate the berries.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.

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Offlinexswaveyx
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101941 - 10/19/08 09:38 PM (15 years, 4 months ago)

Quote:

derx said:
It costs more up front for the bags and the impulse sealer but they are MUCH easier to use, especially when you're trying to do bulk production.  $20 for 100 jars sounds like a steal. 

If you inoculate each jar at 4 different sites, that leads to 4 variables for error each quart.  multiplied by 80 quarts is 320 chances to make an error. 

Now, if you made 2 quart bags and inoculated each bag one time with 1ml of spore solution, you leave only 40 chances for error to make the same amount of substrate.

Shaking up half colonized bags is much easier than shaking up a half colonized jar.



PS.  you can still use rye.  I said grains and that includes rye, WBS, corn, whatever you want.




I dont see the sealer on that site...how much is that? I thought there would be some other way to close them... damn. 20 bucks is a steal for the jars your right, but a lot more hassle i think. This is why i decided to post tonight before i bought anything else. I have the coir/verm and rye...and proper setup. Just need spores and a plan...


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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Offlinexswaveyx
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101955 - 10/19/08 09:42 PM (15 years, 4 months ago)

Quote:

derx said:
I wouldnt reccomend making 4 quart bags until you have your method down precisely.  Start small and then scale up.  Make a few 1quart bags and if that is successful, scale up to 2, then 3... etc...

The chances for wet spot developing increases ten-fold when having 4quarts of wet ryeberries sitting in 1 bag.  Thats why it's key to not overhydrate the berries.




What? are you saying theres different size bags, or that i shouldnt fill it up? So if i made 2 quart bags, then how many could i PC at once? im shooting for like 75% success rate here... i have had contams and built a proper glovebox thats completely sealed and pretty much solved those problems...so i decided i want to do something a little bigger.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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Invisiblederx
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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101959 - 10/19/08 09:43 PM (15 years, 4 months ago)

Lana sells them on her site. 
Impulse sealer

You can also buy them very cheaply on certain auction websites. 
(sorry lana)


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9101966 - 10/19/08 09:43 PM (15 years, 4 months ago)

Quote:

xswaveyx said:
will be doing 14 jars a day and trying to do it all as close together as we can

I know it would be easier to knock up a jar of rye and let it colonize, then spread to other jars.... but thats not what im trying to do




but that's the quickest method to achive your goals.  overall times is much better when you can do everything at once like inoc all jars at once.  buy the 4 strains you want in syringes unless your sterile procedures are really good for making a syringe from print.  four syringes are cheaper than 4 prints :wink:

Quote:

gianimon said:
i do about 70-80 jars at a time.  this is how you should do it IMO.  or by spawn bags, my next purchase :wink:

1. sterilize a first round of 8, 1/2 pint jars.  only put a little WBS, etc in each jar, 1/8-1/4 full.  we use a little spawn so it will colonize faster to 100%.

2. knock up your 8 jars with like 1/2cc each.  2 jars per strain incase of contamination. 





Quote:

xswaveyx said:
You only use 1/2cc on a quart jar? Geeze... ive been using 1cc on my jelly jars... 1/4 in each hole. So then i should put one hole directly in the middle, and inject there?

Let me get this straight

Knock up 8 quarts, 2 of each spawn




no.  8 1/2 pints.


Quote:

xswaveyx said:
Let fully colonize
break it up, spray in 30 cc water, shake up, draw back again
Knock up all other jars with the water aquired

right?




yes.  Agar did this too with the "grain to LC" thread, RR has mentioned this method before as did some other guy.


Quote:

xswaveyx said:
The way i see it, i have 2 16 qt pc's... 7 fit in one...so that 14 i can do at a time, thats a week worth of Pc'ing everyday..




yes.  and it will take that long plus maybe a week or two on the outside for the 1/8-1/4 full 1/2 pint jars to colonize fully :wink:  i get full colonization from MS in about 10-14 days in most cases.

will both PCs fit on your stove at the same time?  have you tried it?  how big are 16 qt PCs?  i guess if you have 4 burners you can do it.  i dont think i could fit two 23 qt PCs on my stove.

i do 20-30 quart jars a day depending upon how awake i am. 

Quote:

xswaveyx said:
not really that bad, especially if i just store them all in bins and knock them up all on the same day. the most ive ever let one sit without inoculation was 3 days and i swore it wasnt gonna work, i was really hesitant about it.




yea they can sit for a while, a few weeks anyway.  ive gone about 2 weeks without issue.


Quote:

xswaveyx said:
Do i have it right? Then ill just have 2 of each jar to play with for the time being i guess huh?




yea you have it right.  but by the time they are colonized will be surprised because youll be PCing like a mofo, lol. 


Quote:

xswaveyx said:
What if i dump all 8 of those jars (4 diff strains) into one monotub and mix it up? would i get a bunch of diff shroomies poppin up everywhere? that would be pretty neat!




i do this too :wink:  when im done taking out the myc water i want i mix the swan with bulk and it works great!!! there is ALOT of myc left in the jars after you take out the water.  but your only doing 8, 1/8 full 1/2 pint jars so not much spawn there.  i do this in 3/4 full quart jars so i have a lot left over.

but this will be one big huge MS grow.  you should look at cloning and then isolation with agar to make a mater slant with or master LC [with food].  a master LC is my preference as its SO much easier to use than a master slant.


--------------------
life is short - drink it up!~~~!

Edited by gianimon (10/19/08 09:45 PM)

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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101967 - 10/19/08 09:44 PM (15 years, 4 months ago)

There are different size bags but I'm only talking about using the "large" size that I provided the link for.

I'm saying you can put different amounts of berries in the bag. 
I'm saying dont start making 4 quart bags until you know you can sucessfully make 2 quart bags.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.

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Offlinexswaveyx
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101976 - 10/19/08 09:48 PM (15 years, 4 months ago)

Quote:

derx said:
There are different size bags but I'm only talking about using the "large" size that I provided the link for.

I'm saying you can put different amounts of berries in the bag. 
I'm saying dont start making 4 quart bags until you know you can sucessfully make 2 quart bags.




i understand. But again... if i make 2 quart bags...how long am i gonna be PCing for? I dont know the proper water procedure for hydrating rye using bags either...since i know nothing about bags obviously. Checkin into that sealer right now...

EDIT: damn the place i used to work at has one of those. Too bad i dont work there still :wink: . 100 bucks is pretty rough, all it does is melt it together. Theres gotta be a cheaper way.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

Edited by xswaveyx (10/19/08 09:50 PM)

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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9101979 - 10/19/08 09:49 PM (15 years, 4 months ago)

xswaveyx,

if you already have quart jars and rye then use them and spawn with it into a bulk sub like hpoo amendments or coir with amendments.  then you can case with 50/50+ tek, 60/40 tek, etc.  or not case if you so choose.  but if you take your 80 jars and put the spawn to bulk you will get a very large yield if you do everything right.

also you need alot more then 10lb of rye for 80 jars.  i use about 20-30 lbs of rye for about 80+ jars with 1.5-1.75 cup per jar, 1 cup is too little for a quart IMO.


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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9101986 - 10/19/08 09:51 PM (15 years, 4 months ago)

bags are the same as jars.  the same moisture in rye for bags as in jars.  bags are just big pliable jars :wink:  well that and you can fruit in them too but id def say go bulk in trays in a fruiting chamber (like a marta) if you already have 80 jars. 


--------------------
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Edited by gianimon (10/19/08 09:53 PM)

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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9101999 - 10/19/08 09:54 PM (15 years, 4 months ago)

Quote:

gianimon said:
xswaveyx,

if you already have quart jars and rye then use them and spawn with it into a bulk sub like hpoo amendments or coir with amendments.  then you can case with 50/50+ tek, 60/40 tek, etc.  or not case if you so choose.  but if you take your 80 jars and put the spawn to bulk you will get a very large yield if you do everything right.

also you need alot more then 10lb of rye for 80 jars.  i use about 20-30 lbs of rye for about 80+ jars with 1.5-1.75 cup per jar, 1 cup is too little for a quart IMO.




I was wondering how much rye i would need. Mycosupply is pretty damn local to me so shipping isnt that much, not a big deal if i gotta buy more. Im sure itll be there before i have time to PC 10 lbs.

I already have Rye, Verm, and Coir...i also have 3 lbs of oyster shell? i do not have jars. I was offered jars, and had not decided if i wanted to take this approach yet. Im planning to put 7 quarts of spawn in each 66 qt monotub.


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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9102006 - 10/19/08 09:56 PM (15 years, 4 months ago)

PS.  There are really easy methods of just adding dry rye to the bags, adding water, sealing them and then cooking them. 
Give them a good shaking after sterilized and inoculate. 
It makes a really easy, no mess, 100% success to producing bulk amounts of spawn.

AFOAF said the rate for rye berries he used was 1500ml of dry rye berries to 1260ml of water in one bag.


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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102010 - 10/19/08 09:56 PM (15 years, 4 months ago)

Quote:

100 bucks is pretty rough, all it does is melt it together. Theres gotta be a cheaper way.




I've never even owned an impulse sealer.  I tie them shut with steel tie wire.  You can get it cheap at any hardware store.
RR


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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9102021 - 10/19/08 09:58 PM (15 years, 4 months ago)

Quote:

derx said:


If you inoculate each jar at 4 different sites, that leads to 4 variables for error each quart.  multiplied by 80 quarts is 320 chances to make an error. 






why would you do that?  only older PF teks suggest the 4 holes deal.  and the OP isnt talking about PF tek.  quart jars use 2 or 3 holes max.  i use 2 holes which are 1/4" each.  one hole is covered with RTV silicon for self-healing injection port and the other hole covered with micro-pore tape.


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Edited by gianimon (10/19/08 10:01 PM)

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Re: Easiest way to knock up bulk quart jars [Re: RogerRabbit]
    #9102030 - 10/19/08 09:59 PM (15 years, 4 months ago)

Quote:

RogerRabbit said:
Quote:

100 bucks is pretty rough, all it does is melt it together. Theres gotta be a cheaper way.




I've never even owned an impulse sealer.  I tie them shut with steel tie wire.  You can get it cheap at any hardware store.
RR




There you are. Thats what i like to hear.... so now im lookin into bags. Does anyone know how many im gonna be able to stick into my 16 qt pc if im usin 2 quarts in each?

If i put in 2 quarts rye, i should put in a little less than a quart water right?... seal bag up, pc it... alchohol wipe the side, knock it up... simple as that right?


--------------------
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9102034 - 10/19/08 10:00 PM (15 years, 4 months ago)

Quote:

derx said:
PS.  There are really easy methods of just adding dry rye to the bags, adding water, sealing them and then cooking them. 
Give them a good shaking after sterilized and inoculate. 
It makes a really easy, no mess, 100% success to producing bulk amounts of spawn.

AFOAF said the rate for rye berries he used was 1500ml of dry rye berries to 1260ml of water in one bag.




there is a blog showing this method with jars too.  everyone ive read about, Agar included, says they got poor success % with that type of method.  in the park of 80-90% success rate :frown:


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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102045 - 10/19/08 10:02 PM (15 years, 4 months ago)

Quote:

gianimon said:
Quote:

derx said:


If you inoculate each jar at 4 different sites, that leads to 4 variables for error each quart.  multiplied by 80 quarts is 320 chances to make an error. 






why would you do that?  only older PF teks suggest the 4 holes deal.  and the OP isnt talking about PF tek.  quart jars use 2 or 3 holes max.  i use 2 holes which are 1/4" each.  only hole is covered with RTV silicon for self-healing injection port and the other hole covered with micro-pore tape.




He mentioned that because i said i use 4 inoculation points for my half pint jars. I guess maybe im the only one that does that anymore? Regardless... failing only makes me want to learn more so after losing a couple jars i worked harder to make a more sterile place to inoculate. Now i have a HUGE tub with full gloves.... 2 on each side so we can use the buddy system while knockin up a ton of jars. Worked awesome.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102053 - 10/19/08 10:04 PM (15 years, 4 months ago)

Quote:

gianimon said:
Quote:

derx said:
PS.  There are really easy methods of just adding dry rye to the bags, adding water, sealing them and then cooking them. 
Give them a good shaking after sterilized and inoculate. 
It makes a really easy, no mess, 100% success to producing bulk amounts of spawn.

AFOAF said the rate for rye berries he used was 1500ml of dry rye berries to 1260ml of water in one bag.




there is a blog showing this method with jars too.  everyone ive read about, Agar included, says they got poor success % with that type of method.  in the park of 80-90% success rate :frown:




Some people have better results doing things differently.  To each and their own.

edit:  I would not use this method for jars.  Jars are much harder to shake up to create a homogeneous mixture.  Which in turn could lead to more water near the bottom and dry rye near the top.  Lots of water = contams.  Dry rye = wont colonize.


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Edited by derx (10/19/08 10:06 PM)

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9102059 - 10/19/08 10:06 PM (15 years, 4 months ago)

Quote:

xswaveyx said:
Quote:

RogerRabbit said:
Quote:

100 bucks is pretty rough, all it does is melt it together. Theres gotta be a cheaper way.




I've never even owned an impulse sealer.  I tie them shut with steel tie wire.  You can get it cheap at any hardware store.
RR




There you are. Thats what i like to hear.... so now im lookin into bags. Does anyone know how many im gonna be able to stick into my 16 qt pc if im usin 2 quarts in each?

If i put in 2 quarts rye, i should put in a little less than a quart water right?... seal bag up, pc it... alchohol wipe the side, knock it up... simple as that right?




if this is your first time growing then i would suggest against bags.  use jars to get the hang of it.  its much better to loose a few jars to contam, etc, then a few bags! 

if you can get 80 jars for $20 then at least by them and keep them or sell those suckers.  12 jars on the market is about $10-15.00. but if i were you i would inoc like 20-30 jars to get the hang of it, get a few flushes going and then go bigger.


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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102090 - 10/19/08 10:11 PM (15 years, 4 months ago)

Quote:

gianimon said:
Quote:

xswaveyx said:
Quote:

RogerRabbit said:
Quote:

100 bucks is pretty rough, all it does is melt it together. Theres gotta be a cheaper way.




I've never even owned an impulse sealer.  I tie them shut with steel tie wire.  You can get it cheap at any hardware store.
RR




There you are. Thats what i like to hear.... so now im lookin into bags. Does anyone know how many im gonna be able to stick into my 16 qt pc if im usin 2 quarts in each?

If i put in 2 quarts rye, i should put in a little less than a quart water right?... seal bag up, pc it... alchohol wipe the side, knock it up... simple as that right?




if this is your first time growing then i would suggest against bags.  use jars to get the hang of it.  its much better to loose a few jars to contam, etc, then a few bags! 

if you can get 80 jars for $20 then at least by them and keep them or sell those suckers.  12 jars on the market is about $10-15.00. but if i were you i would inoc like 20-30 jars to get the hang of it, get a few flushes going and then go bigger.




Understandable... however i started with cakes and worked my way up. Im now going to do monotubs. Im just going to do more than 1 at a time, thats why im doing so many.

If someone else can do a bag, i can do a bag. Ill never get it right if i dont try it. Ill start out with 2 quart bags.

If i dont need 100 jars, im not going to buy them. They will be harder to store than bags, and a lot more tedious to break up (when you have this many). I dont just need 100 quart jars laying around, if im not going to use them now i wont use them later.


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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9102110 - 10/19/08 10:17 PM (15 years, 4 months ago)

thats cool but trust me ive done way more jars than your thinking about so i know the work involved. and it can be a real pain in the  ass, lol.  jars arnt only for monotubs, you can spawn into bulk into a fruiting chamber/closet/martha (what ever you want to call it).

breaking up a jar is a snap, 5-10 whacks on a bike tire (thanks RR! :smile: ) and its done.  alot quicker than man handling a bunch of bags IMO. 

but i do think bags are better for large bulk grows.  i just wish bags were more environmentally friendly...

GL


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Edited by gianimon (10/19/08 10:18 PM)

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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102143 - 10/19/08 10:25 PM (15 years, 4 months ago)

Quote:

gianimon said:
thats cool but trust me ive done way more jars than your thinking about so i know the work involved. and it can be a real pain in the  ass, lol.  jars arnt only for monotubs, you can spawn into bulk into a fruiting chamber/closet/martha (what ever you want to call it).

breaking up a jar is a snap, 5-10 whacks on a bike tire (thanks RR! :smile: ) and its done.  alot quicker than man handling a bunch of bags IMO. 

but i do think bags are better for large bulk grows.  i just wish bags were more environmentally friendly...

GL




I know they arent only for montoubs man...cmon now. I was just sharing what i have planned. I can man handle a bunch of bags, i have an extra set of hands or two so it shouldnt be that big of a deal.

Id rather not have that bad history of knocking up hundreds upon hundreds of jars. Id rather talk bout how i started with 2 qt bags and moved up haha. Will be ordering 100 bags, in place of my 100 jars. Now...being that i have 2 qt bags... thats 10 bags per strain. I only need 1 syringe of each strain then correct? This wont be an issue after i have mushrooms of each strain obviously so this is a one time buy...

Penis envy, cambodian, texas, equador. Thats the plan. Ive grown PE and PESA, and if i have extras i might start using pesa as well.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9102172 - 10/19/08 10:31 PM (15 years, 4 months ago)

Quote:

xswaveyx said
I know they arent only for montoubs man...cmon now. I was just sharing what i have planned. I can man handle a bunch of bags, i have an extra set of hands or two so it shouldnt be that big of a deal.




i wasnt trying to be rude about the monotubs.  i dont know your level of knowledge.  sorry.  i sure wish i had some helpers would make my life much easier!  later :smile:


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Edited by gianimon (10/19/08 10:32 PM)

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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102181 - 10/19/08 10:34 PM (15 years, 4 months ago)

Quote:

gianimon said:
Quote:

xswaveyx said[/]b
I know they arent only for montoubs man...cmon now. I was just sharing what i have planned. I can man handle a bunch of bags, i have an extra set of hands or two so it shouldnt be that big of a deal.




i wasnt trying to be rude about the monotubs.  i dont know your level of knowledge.  sorry.  i sure wish i had some helpers would make my life much easier!  later :smile:




Sorry. You should know my level of knowledge after reading the first page haha. I just decided monotubs will be the BEST setup for my personal choices. I also now decided to go with bags. I will stick with my rye and coir though haha. Tons of good knowledge here...im lovin it.

Although i asked like 10 times already how much ill be able to PC at once, i guess nobody has an answer.


--------------------
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Re: Easiest way to knock up bulk quart jars [Re: gianimon]
    #9102223 - 10/19/08 10:46 PM (15 years, 4 months ago)

buy your impulse sealer HERE they are only 40 bucks. tested, myco bag approved. :thumbup:

get the bags from mycosupply.com i like the large bags.
see how much they want for their impulse sealer while your their  :wink:

and follow THIS tek for preparing the bags.

in my opinion i would say dont buy the stuff for this current grow. go with your jars for now but research the information about bags and give it a go in the future. try to follow that tek to a T about preparing spawn bags. its weird at first and might seem complicated as far as folding the top of the bags and getting them in the pressure cooker but once you get it down youll be happy you learned this. I hate cleaning jars and then storing them. they suck ass but work well. read the tek I linked you to. he will explain why you should go with bags. goodluck i hope this advice helps for your future projects.

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Re: Easiest way to knock up bulk quart jars [Re: tripchip]
    #9102261 - 10/19/08 10:53 PM (15 years, 4 months ago)

Quote:

tripchip said:
buy your impulse sealer HERE they are only 40 bucks. tested, myco bag approved. :thumbup:

get the bags from mycosupply.com i like the large bags.
see how much they want for their impulse sealer while your their  :wink:

and follow THIS tek for preparing the bags.

in my opinion i would say dont buy the stuff for this current grow. go with your jars for now but research the information about bags and give it a go in the future. try to follow that tek to a T about preparing spawn bags. its weird at first and might seem complicated as far as folding the top of the bags and getting them in the pressure cooker but once you get it down youll be happy you learned this. I hate cleaning jars and then storing them. they suck ass but work well. read the tek I linked you to. he will explain why you should go with bags. goodluck i hope this advice helps for your future projects.




$40 isnt bad for a sealer, but like RR said im just going to tie them shut good with metal. I dont see anything getting in there, i dont need another piece of equipment around that i honestly wont use except for this purpose.

Mycosupply has bigger bags, but they are pretty damn expensive. Since im not going to fill my bags up to start with, i think ill just buy the smaller, cheaper ones. And they dont have a shop to go to, i still have to order offline like everyone else... they are in industrial park so only trucks can drive through. Sucks, i was excited that i wouldnt have to pay to ship all my supplies.But oh well, they are a good sponsor and i bought all the rest of my stuff from them. Im sure ill be using big bags soon enough.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9104651 - 10/20/08 03:08 PM (15 years, 4 months ago)

So... how often should i shake up my bags? Id imagine i should give it about 5 days to start, then mix it up real good, but then how often after that until full colonization? I read that they colonize pretty fast...which is pretty exciting!


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9110471 - 10/21/08 03:33 PM (15 years, 4 months ago)

inoculate them with aprox 1-2ml of solution.  AFOAF always used 1.  Do not shake the bag up.  Let that colonize until you get a small baseball size chunkj of mycelium, break it up well and shake up the bag to evenly disperse it.  From that point, it will colonize very quickly.  When it gets near 90% colonized, break it up again and shake it up. 
You can easily have fully colonized bags in 11 days using LC


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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9111736 - 10/21/08 07:11 PM (15 years, 4 months ago)

I only ordered 10 pounds of rye. i will need around 20 more pounds for what i want to do. Is it a bad idea to use birdseed? I saw a 20 lb bag advertised for 7.99. Sounds like a really cheap alternative.Good/bad idea?

EDIT:another question... does one keep there fully colonized LC in a fridge for storage? Just something i havent read anywhere...what to do if you have too much LC for what you need, and want to store it (obviously it can be done as i see these huge ass jars some people are making).


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

Edited by xswaveyx (10/21/08 07:23 PM)

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9112106 - 10/21/08 07:59 PM (15 years, 4 months ago)

As i sat here and thought harder. I have one little mushy still growing on a cake in there... of PE. Since it wont print... can i make an LC using live tissue? if not im going to try to print it and use whatever moisture it leaves and cross my fingers i guess. Worth a shot.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9113621 - 10/22/08 12:46 AM (15 years, 4 months ago)

wild bird seed is great and its easy i started with it then went to rye and am now back with WBS.:cheers:


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Re: Easiest way to knock up bulk quart jars [Re: gethyn]
    #9113637 - 10/22/08 12:49 AM (15 years, 4 months ago)

Get real sterile and open it up at the base inside cut away part of the center tissue and lc if your clean and quick youll have more to play with my friend.:cheers:


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Re: Easiest way to knock up bulk quart jars [Re: gethyn]
    #9115031 - 10/22/08 11:18 AM (15 years, 4 months ago)

Quote:

gethyn said:
Get real sterile and open it up at the base inside cut away part of the center tissue and lc if your clean and quick youll have more to play with my friend.:cheers:




Does it matter where i cut? should i try to get to the center of the mushroom where it hasnt touched outside air and use that part for an LC or can i just whack a slab off of it anywhere? Im doing this today...well the jars today atleast.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9115143 - 10/22/08 11:37 AM (15 years, 4 months ago)

He just told you to cut the center tissue.  By that he means not exposed to air side.


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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9115421 - 10/22/08 12:38 PM (15 years, 4 months ago)

this is how i did mine

soak polyfil with alcohol sprayer, flame needle, wrap needle in alcohol paper towel, quickly shove into the polyfil, inject LC, pull out and wrap in alcohol paper towel.....no contams as of yet. i would shit myself trying to noc 100 jars in a glovebox.


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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9117142 - 10/22/08 05:32 PM (15 years, 4 months ago)

Quote:

derx said:
He just told you to cut the center tissue.  By that he means not exposed to air side.




Just trying to make sure thats what he meant. Sorry.

I didnt want to start a new topic but... on the subject of popcorn...since ive been reading about wbs. I see samsclub has a 50 lb bag of popcorn for $23. Ive read the tek on popcorn but says nothing about using bags at all. If i were to use bags... popcorn doesnt expand right? So i would have to fill each bag up with 2 qts of popcorn to make 2 qts of spawn correct? I think im going to use the 10lb of rye, and then try popcorn...since it seems a lot less of a hassle than birdseed from the teks i read. Can someone clear that up?:stoned:

EDIT: Is corn starch a bad idea for an LC?Its all i have available atm.:crazy:


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Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

Edited by xswaveyx (10/22/08 06:18 PM)

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Offlinegofudgeyourself
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Re: Easiest way to knock up bulk quart jars [Re: derx]
    #9118304 - 10/22/08 09:17 PM (15 years, 4 months ago)

this is how i did mine

soak polyfil with alcohol sprayer, flame needle, wrap needle in alcohol paper towel, quickly shove into the polyfil, inject LC, pull out and wrap in alcohol paper towel.....no contams as of yet. i would shit myself trying to noc 100 jars in a glovebox.


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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9118365 - 10/22/08 09:28 PM (15 years, 4 months ago)

Quote:

xswaveyx said:
Well, everything is in place except for my spores. im knocking up 80-100 quart jars, im guessing closer to 100 since we have them.... but im going straight from spores. I have tons of PESA prints that would easily do the job, but the plan was to have 20 of each strain (4 straings), and then make some extras. So pretty much i need to make 25 quarts out of each strain. What would be the easiest approach? Buy a print, and liquify it down.... buy 8 syringes? How much do i really need to get this going?I know it would be easier to knock up a jar of rye and let it colonize, then spread to other jars.... but thats not what im trying to do, will be doing 14 jars a day and trying to do it all as close together as we can. Thanks




Dude don't waste your time waiting for 100 jars of multispore to colonize.  Do a few of each, if you don't have contaminants do GRAIN TO GRAIN to fresh jars at a ratio of 10:1.  10 new jars to 1 colonized healthy jar.

The G2G jars will be done fucking quick and you'll probably have a better contaminant rate.

You only need one syringe of each strain.

Just start with syringes and do G2G.

:2cents:

Edited by drwatson (10/22/08 09:29 PM)

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Re: Easiest way to knock up bulk quart jars [Re: drwatson]
    #9118381 - 10/22/08 09:32 PM (15 years, 4 months ago)

read 2 posts up? :confused:


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9118497 - 10/22/08 09:48 PM (15 years, 4 months ago)

It would suck to lose 100 jars to a contaminated LC that's all I'm saying, multispore or not you can't see contaminates in an LC.

you could use this to make a viable, not contaminated, clone of your fruit body to inoculate grain masters then do grain to grain.

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8803460/an/0/page/1

I know it's not the easiest but I think it's probably the best way to get the results you want.

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Re: Easiest way to knock up bulk quart jars [Re: drwatson]
    #9118820 - 10/22/08 10:36 PM (15 years, 4 months ago)

Quote:

drwatson said:
It would suck to lose 100 jars to a contaminated LC that's all I'm saying, multispore or not you can't see contaminates in an LC.

you could use this to make a viable, not contaminated, clone of your fruit body to inoculate grain masters then do grain to grain.

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8803460/an/0/page/1

I know it's not the easiest but I think it's probably the best way to get the results you want.




you obviously just read the first post and responded without seeing what others have said already. im not using jars anymore. thats why i just asked those questions like 6 posts up.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9118840 - 10/22/08 10:39 PM (15 years, 4 months ago)

oh okay.  well good luck then!

no i didn't read all three pages and just answered the original post.

really my main concern was pushing g2g over lc but to each his own

edit: and you could still do grain masters and g2g with bags

Edited by drwatson (10/22/08 10:43 PM)

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Re: Easiest way to knock up bulk quart jars [Re: drwatson]
    #9118944 - 10/22/08 10:57 PM (15 years, 4 months ago)

im more focused on how much popcorn i need to use to make 2 qts of spawn :wink: and if i can do it in bags like rye. I read popcorn tek using jars, just makin sure im doin it right before i do it


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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Re: Easiest way to knock up bulk quart jars [Re: xswaveyx]
    #9120186 - 10/23/08 08:35 AM (15 years, 4 months ago)

I would try another grain/grains. Wbs or grain would be my suggestions. I've had terrible luck with corn and I know a lot of others have also. I lean towards wbs because of price and availability. Good luck

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Re: Easiest way to knock up bulk quart jars [Re: petridish]
    #9120747 - 10/23/08 11:15 AM (15 years, 4 months ago)

wbs looks like a lot more work, and i have read a ton of ppl having trouble with the right moisture/seeds germinating completly.


--------------------
Delta-9-tetrahydrocannabinol - or THC, the active chemical in weed - is in the same family of organic molecules as anandamide, a chemical found in the human brain that is associated with feelings of bliss such as those released during sex. So on a molecular level, getting laid and getting stoned aren't too different.

My Growlog with Redboy,Amazon,and Brazilian

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