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OfflineSuicideCommando
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First batch of PF Cakes, some questions...
    #9097689 - 10/18/08 08:27 PM (15 years, 5 months ago)

So my first attempt at this went underway about 2 weeks ago. I started with 5 jars then, and inoculated another 5 a few days ago. The first 5 seem do be doing ok. After about a week I saw little balls of mycelium forming at the injection sites. They have gotten bigger and are silky looking and fluffy white, getting bigger every day, no sign of contams at all, which is a relief because I didnt use a glovebox, I just inoculated in my very small bathroom after spraying everything down with Lysol. Though 1 jar has done absolutely nothing. I know I didnt put the exact same amount of solution in each jar, some got 1cc, some got slightly more, maybe that one got slightly less, I dunno, but it wouldnt have been that much less,  and after just over 2 weeks it has no signs of life. Other jars have several big and growing clumps of myc but this jar, nothing!
Could this be just because I injected less solution into it, and its just going THAT slowly, or could it be something else?
Also I just want to make sure Im doing this the best way I can. Ive been keeping the jars on my bookshelf since inoculation. They get very low indirect sunlight that filters through my basement apartment window curtain and they are at room temperature, not exactly sure what that is but I have central heating and I can sit here without a shirt and be comfortable enough, not hot or cold. I wonder, since some jars seem to be going slower than other n all, are these ok conditions, or should I change something? Increasing the temp of the jars is not possible without wrapping them with clothes or something, so I hope I dont have to do that.
The strain is Ecuadorian btw, if that makes a difference.


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Offlineveda_sticks
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Re: First batch of PF Cakes, some questions... [Re: SuicideCommando]
    #9097725 - 10/18/08 08:32 PM (15 years, 5 months ago)

Strain doesnt make a difference. Colinising at room temperature is fine. The difference between 75f and 80F is minimal.

Reasons for slow colinisation are usually

Overly wet substrate
Inadequate gas echange (dont cover the inoc holes)


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
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Offlineblackclw
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Re: First batch of PF Cakes, some questions... [Re: veda_sticks]
    #9097757 - 10/18/08 08:37 PM (15 years, 5 months ago)

Just wait a week or two. I had similar situation. One cake started (visibly) growing on around day 20.

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OfflineSuicideCommando
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Re: First batch of PF Cakes, some questions... [Re: blackclw]
    #9097779 - 10/18/08 08:42 PM (15 years, 5 months ago)

Well I haven't covered the holes. I had them taped with masking tape, then just put the syringe through the tape for inoculation, then left them just like that.
The recent 5 jars definitely look more wet than the first 5, so much so that it was the first thing I thought when I looked at them a few hours after sterilization. If that is going to cause those 5 to go much slower is there something I can do to fix that?


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I have never killed a man, but I have read many obituaries with great pleasure.

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OfflineRogerRabbitM
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Re: First batch of PF Cakes, some questions... [Re: SuicideCommando]
    #9097816 - 10/18/08 08:53 PM (15 years, 5 months ago)

Remove the masking tape from the holes.
RR


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OfflineSuicideCommando
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Re: First batch of PF Cakes, some questions... [Re: RogerRabbit]
    #9097848 - 10/18/08 09:01 PM (15 years, 5 months ago)

masking tape removed :thumbup:


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I have never killed a man, but I have read many obituaries with great pleasure.

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