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OfflineArose Chaos
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First try methods and ideas?
    #9093652 - 10/17/08 08:32 PM (16 years, 3 months ago)

Welp im goin for my first pf-tek grow! so i figured id post how i plan on doing it and if anyone has any ideas, comments or suggestions for me please post because i really dont want to screw this up....

First thing done already - boiled distilled water in non-teflon pot for 5 min then got 10 cc's in 2 syringes filled up, and at this very moment are sitting on my alcohol-rubbed-down counter for the next 12 hours to cool

-when they are cool i plan to get a shot glass rubbed down in alcohol
then take the print in the baggie, scrape it out into the shot glass, squirt the water into it from the syringe, mix around then suck back up      then syringes are complete

-jars- bought brf and vermiculite, then kerr half pint wide mouth jars, plan on mixing 1/4 cup of brown rice flour, 1/2 cup of vermiculite ''medium grade'', 60 cc's water then mixing together.
then filling half pint jars with the mix, leaving 1/2 inch of dry verm on top

-then putting in pressure cooker for 20 min 12 psi, all the while putting tape over the ''4'' holes in each lid, covered in tin foil

once done put em away till they start goin!

this is what i got so far please give any advise or suggestions

thanks :smile:


--------------------
<<WOW
"There is nofhing noble in being superior to your fellow man;
true nobility is being superior to your former self" -Hemingway

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InvisibleJean-Luc Picard
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Re: First try methods and ideas? [Re: Arose Chaos]
    #9093840 - 10/17/08 09:06 PM (16 years, 3 months ago)

when you load the jars...load them loosely all the way to the top...use a butterknife or ur finger to level it off. Then gently tap the bottom of the jar on the palm of ur hand a few times. What should happen is the brf/verm should tamp down about 1/2 inch. then take a dry paper towel and wipe the inside of the jar rim to clean all of the inside glass exposed above the brf/verm. after all of this is done you may add the dry verm.

the reason for doing this is to make sure the level of dry verm is consistant, and wiping the inside glass removes any trace of nutrients from the contam barrier, thereby increasing ur chances for success.

also dont bother with the tape over the holes. The dry verm layer is enough of a contam barrier and once germination occurs you will need to remove it anyway so the mycelium can get enough oxygen

once the jars are PC'd and cool, you want to inoculate with ur spores. Take a lighter and heat the needle until it glows red. then wipe it down with an alcohol soaked cloth. Leave the need wrapped in the cloth for now. Remove the tinfoil over ur jar, then in one swift motion remove the need from the cloth and insert into one of the holes in the jar lid. Inject about 1/4 to 1/2 cc per hole or 1 to 2 cc per jar. Do Not incubate over 80 degrees.

once they are completely colonized aka covered in white, remove them from the jar and submerge them underwater for 12 to 24 hours. Then roll them in verm and mist liberally to moisten the new verm layer on each cake. now put them somewhere with 70-75 degree temperatures, indirect light in a 12 on 12 off schedule, 85% or better relative humidity, and plenty of air exchange and watch the magic happen. Harvest them once the veil tears from the cap, exposing the gills.

hope this helps :grin::thumbup:


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The universe is under no obligation to make sense to you - NDT

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OfflineArose Chaos
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Re: First try methods and ideas? [Re: Jean-Luc Picard]
    #9093905 - 10/17/08 09:19 PM (16 years, 3 months ago)

hey ya thanks for the advise!

one thing im confused as hell on is the pressure cooker...

theres no real adjustment knob to the cooker its a 8 quart 10 psi mirro, so how would i cook this, anyone know?


--------------------
<<WOW
"There is nofhing noble in being superior to your fellow man;
true nobility is being superior to your former self" -Hemingway

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InvisibleJean-Luc Picard
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Re: First try methods and ideas? [Re: Arose Chaos]
    #9095299 - 10/18/08 05:22 AM (16 years, 3 months ago)

all you do is put ur jars in and fill with water until the water level is about halfway up the jar. Put the lid on but not the weight, leave the stem at the top open. Heat on high until a semi-steady stream of steam is coming from the stem at the top, then add the weight to the stem to block the flow of steam and cut down on the heat just a little bit. after this you need to read the manual. I have a presto and i'm  95% sure that you wait until the weight on top begins to rock back and forth, but again this is not a presto ur working with so i would double check. Most of the cheaper pressure cookers just run at either 10 or 15 psi so just cook them for 30 minutes or so. Start your cooktime when the weight begins to rock back and forth.


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The universe is under no obligation to make sense to you - NDT

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InvisibleJean-Luc Picard
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Re: First try methods and ideas? [Re: Jean-Luc Picard]
    #9095300 - 10/18/08 05:23 AM (16 years, 3 months ago)

oh yea i forgot to tell you to cut the heat back to medium when the weight begins to rock back and forth sorry


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OfflineArose Chaos
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Re: First try methods and ideas? [Re: Jean-Luc Picard]
    #9095748 - 10/18/08 10:41 AM (16 years, 3 months ago)

yep got it all covered 12 jars sitting on my counter sterilized and covered by a big rubbermaid tub so no air can get into them...

so im worried that when i lift up the tub to get under and inject i may let some bad stuff in... should i just layer by kitchen down with oust and alcohol?

and i may have made a dumb mistake.... how do i know where the holes in the lid are? theres three layers of foil so when i take layer one off how do i know where to poke the needle through if i cant see the holes..? hopefully not a dumb question

thanks in advance


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<<WOW
"There is nofhing noble in being superior to your fellow man;
true nobility is being superior to your former self" -Hemingway

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Offlineveda_sticks
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Re: First try methods and ideas? [Re: Arose Chaos]
    #9095799 - 10/18/08 11:02 AM (16 years, 3 months ago)

www.mushroomvideos.com ;                                        

watch all 4 pf tek videos. theres also a video on makking spore syringes. distilled water is fine for spore syringes. use regular tap water for your substrate if its drinkable quality.

make sure you use a glovebox when making prints and inoculating your jars. follow the teks to the t and you willl be fine.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineArose Chaos
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Registered: 08/25/08
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Re: First try methods and ideas? [Re: veda_sticks]
    #9095968 - 10/18/08 12:07 PM (16 years, 3 months ago)

alright got em all inoculated, feels kinda sketchy, i tipped the rubbermaid container on its side and used that as kind of a 4 sided glovebox with the one opening for me to work in. i poked through the foil and did 1 cc per jar 1/4 cc per hole, and lightered the needle down and rubbed alcohol after every second jar.

put all the containers in the rubbermaid container put some coats, sweaters and jackets over em to keep warm i guess, so now i just wait to see white or contam?

thanks for all the help guys


--------------------
<<WOW
"There is nofhing noble in being superior to your fellow man;
true nobility is being superior to your former self" -Hemingway

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OfflineVermonster420
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Re: First try methods and ideas? [Re: Arose Chaos]
    #9096093 - 10/18/08 12:54 PM (16 years, 3 months ago)

No more need for foil, the dry verm layer will filter.  The sweaters may restrict FAE, I'd suggest removal and using a heating pad to keep the jars around 80 degrees.  I simply place a heating pad in a dresser drawer and put all my inoculated jars in the drawer. 
I also read the cultivation forum and contamination forum for 2 months before starting.  I feel I have earned the e-quiv of 2 semesters of mycology by doing so, this forum is amazing in it's educational quality.  Use it!  Most questions can be self-answered if you study study study!


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To thine own self be true.
That which does not kill me makes me stronger.
Ass...it's the NEW pussy!
"Gungah-DeGungah"

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Offlineveda_sticks
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Re: First try methods and ideas? [Re: Vermonster420]
    #9096116 - 10/18/08 01:02 PM (16 years, 3 months ago)

alcohol every second jar?? alcohol is not a steriliser. flaming red hot is. and you should flame before inoculating each jar, waiting a few seconds to allow the needle to cool


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

Extras: Filter Print Post Top
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Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)


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