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OfflineIcAnFeeLthErain
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Registered: 05/24/08
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Liquid Culture Syringes
    #9095896 - 10/18/08 11:42 AM (16 years, 3 months ago)

What up y'all.

The other day I had a LC jar come out of the PC w/ some sediment :mad2: I forgot to filter it.

So I just made LC spore syringes.  I pulled up some of the LC without disturbing the sediment (assuming all the bad stuff is at the bottom).  And then I took out my shot glass and spore print and prepared the syringes as you would making a normal syringe...except these syringes have nutrients!  I checked back two days later and lo and behold...mycelium.

has anyone else tried this?


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"We are here to awaken from the illusion of our separateness" -Thich Nhat Hanh

Take your sweet time, everything is a once in a life time experience.

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Offlineveda_sticks
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Re: Liquid Culture Syringes [Re: IcAnFeeLthErain]
    #9096240 - 10/18/08 01:48 PM (16 years, 3 months ago)

2 days later? more likely to be mould mycelium. its not a great method. lack of gas exchange and a waste of spores.                                 

use something better for lc like malt...... or better yet start out on agar.


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OfflineIcAnFeeLthErain
How ya like your Chang's Sauce?
Male


Registered: 05/24/08
Posts: 331
Loc: Bright Side of the Road
Last seen: 13 years, 7 months
Re: Liquid Culture Syringes [Re: veda_sticks]
    #9096313 - 10/18/08 02:05 PM (16 years, 3 months ago)

yeah i use malt, first batch had great success and was used to colonize rye quarts.  this second batch was bunked due to sediment, but I see what you mean by the lack of gas exchange.  Guess that's why I saw all that condensation on the inside of the syringes when I went back to check on them.  My last LC showed prominent signs of growth in two days, I don't see why this same MS wouldn't.


--------------------
"We are here to awaken from the illusion of our separateness" -Thich Nhat Hanh

Take your sweet time, everything is a once in a life time experience.

Cakes?  No Thanks.

Extras: Filter Print Post Top
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