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Offlinegofudgeyourself
Student
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Registered: 09/01/08
Posts: 710
Loc: Flag
Last seen: 13 years, 7 months
altering working glovebox.
    #9088008 - 10/16/08 06:38 PM (16 years, 3 months ago)

so here is my glovebox. (i dont have a camera, am poor, so bare with me)

its a 10 gallon aquarium on its side, the lid is 1/4" plexiglass with 2 holes cut in it. in the holes i put 2 PVC couplings with rubber gloves siliconed on...when i need to work i have to DUCT TAPE THE ENTIRE TOP to get still air, otherwise the lid is cut in a way where if the gloves move in and out it stirs air in the entire box....

my question is should i just cut the gloves off? i think if i cut the wrist/hand part of the gloves off and just silicone the lid down it would make my life alot easier. it would be like having mini rubber tyvek sleeves.

it works awesome right now, im just sick of duct taping the lid every fucking time i want to work...i only do PF jars, printing and LC shit in it..would it be better to have a pressurized perfectly still box that is a pain in the ass or an easy to use "still" air box...although cleaning would be a pain.

advice?


--------------------
"believe it if you need it, or leave it if you dare."

"turn all the hate in the world, into a mocking bird. make it fly away" :peace:

Edited by gofudgeyourself (10/16/08 06:40 PM)

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OfflineT2K_Rusty
Rusty Shackleford
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Registered: 10/08/08
Posts: 342
Last seen: 16 years, 1 month
Re: altering working glovebox. [Re: gofudgeyourself]
    #9088173 - 10/16/08 07:14 PM (16 years, 3 months ago)

Honestly, you don't need a glovebox for any of that. Sure it will give you some insurance, but in reality unless you're opening jars or something, you don't need one. Micropore tape will suffice for your needs.




Do not get me wrong, a glovebox is better than not having one, in any circumstance.  There's just a very, very low chance you will contaminate anything you do without one, as long as you follow sterile proceedure (flame the needle, keep the whole place clean in general).

Other than that, the prints are the thing you have to be most carefull with. LC is always a crapshoot no matter how sterile, unless you make it from a good agar wedge.

Just take the prints, and put something over them like this to prevent any direct inflow of airborne spores.

Anyone that tells you you need more than that, is so dead wrong. I've never had 1 single contamination with any home made syringe, out of several hundred.  The spores are exposed to open air before the cap is cut anyways.... this just prevents crap from settling with them.

Also don't take prints off of a cap that has touched anything, including another cap. A glove box still wouldn't do anything for the sterility of that scenario.


--------------------
Sh sh shaw!


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