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rlucas
Lurkin


Registered: 10/01/08
Posts: 184
Loc: Anywhere but here
Last seen: 11 years, 9 months
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Need Advice
#9086606 - 10/16/08 01:09 PM (16 years, 3 months ago) |
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I am about to start growing mushrooms for the first time. I got P.Cubensis B+ strain. I am gonna follow the pf tek for inoculating the jars but i plan on using a pmp instead of the fruiting chamber described in the pf tek. If i wanna use cakes is there a different tek that is better then pf tek?
Any other advice for starting out would be appreciated Sorry if its already been answered somewhere else
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nenl
Strangest


Registered: 07/16/08
Posts: 683
Last seen: 13 years, 2 months
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Re: Need Advice [Re: rlucas]
#9086623 - 10/16/08 01:11 PM (16 years, 3 months ago) |
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pf tek from www.mushroomvideos.com is the easiest and most reliable method for brf cakes.
good luck
-------------------- hehe
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SickShroomer
Retired Shroom Master



Registered: 05/25/06
Posts: 361
Loc: Midwest
Last seen: 4 years, 2 days
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Re: Need Advice [Re: nenl]
#9086642 - 10/16/08 01:14 PM (16 years, 3 months ago) |
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If its your first time PF-Tek is the best..
BTW, PF-Tek is synonymous with cakes.. So the only way to do PF-Tek is to make cakes!!
-------------------- Going to miss the PNW gatherings.
I'm not here trying to make anyone feel stupid or to make myself feel good by putting ppl down. I am just here to help fellow shroomerites in there grows. I do this to keep my own knowledge fresh, and to help everyone succeed!
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rlucas
Lurkin


Registered: 10/01/08
Posts: 184
Loc: Anywhere but here
Last seen: 11 years, 9 months
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thanks also, is vermeculite and brf the best things to use for the cakes? And is it ok to use a bic lighter to sterilize the needle on the syringe?
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nenl
Strangest


Registered: 07/16/08
Posts: 683
Last seen: 13 years, 2 months
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Re: Need Advice [Re: rlucas]
#9086664 - 10/16/08 01:19 PM (16 years, 3 months ago) |
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yes and yes.
verm is there to hold moisture for the myc. brf is the food and does not need a pressure cooker to sterilize. (do as the video commands! )
bic will get pretty hot by the 5th jar, so if you have anything that's designed for a constant flame - even a candle might do it. as long as the needle gets red hot you're good.
-------------------- hehe
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Need Advice [Re: nenl]
#9087214 - 10/16/08 03:27 PM (16 years, 3 months ago) |
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I've seen WBS Flower used instead of BRF successfully by a good grower on this sight. It could lead to less flushes but showed good yields.
oh and candles are good I use em without contaminants.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
Edited by ScavengerType (10/16/08 03:29 PM)
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dead
grateful



Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 10 years, 2 months
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I'm still using rye flour without a PC with no contams.
-------------------- "The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
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Re: Need Advice [Re: dead]
#9087532 - 10/16/08 05:00 PM (16 years, 3 months ago) |
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No.
Candles also leave black soot residue behind. Get an alcohol lamp, or an oil lamp and dump out the oil... rubbing alcohol or Everclear work great inside of them and burns off nice and clean
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Need Advice [Re: P.Menace]
#9087865 - 10/16/08 06:08 PM (16 years, 3 months ago) |
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As is my understanding soot is not a vector for contaminants it's just dirty. Either way wiping it down with an alcohol cloth before using it will prevent the soot from being tracked onto anything.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
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thats too much extra work, if you inoculating 200+ jars like this
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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rlucas
Lurkin


Registered: 10/01/08
Posts: 184
Loc: Anywhere but here
Last seen: 11 years, 9 months
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Re: Need Advice [Re: P.Menace]
#9088048 - 10/16/08 06:47 PM (16 years, 3 months ago) |
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ok thanks cant wait to start:)
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Need Advice [Re: rlucas]
#9088461 - 10/16/08 08:08 PM (16 years, 3 months ago) |
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P.menace Haven't you learned to G2G yet? And I somehow doubt the OP was inoculating that much. If your doing that many cakes usually you could just do bulk trays much more easily or tubs if you need to test many different strains at a time.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
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Yes I have learned that G2G is a risky process involving super sterility, which I have. and that was actually a picture from Christmas of 07. I grow in bags now, jars are too small and time consuming for me, I like to see big mushrooms too
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Need Advice [Re: P.Menace]
#9088651 - 10/16/08 08:47 PM (16 years, 3 months ago) |
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oh yea that's right you do, do bags. IMO though G2G requires some alcohol, a paper towel, a smooth metal spoon (perhaps butterknife too in case things get harry), gloves, Tyvek Wrist Sleeves (optional), and a glovebox. I've never had a G2G contaminant with this equipment. I wouldn't call this Super sterile, it's not like you need a flowhood.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
Edited by ScavengerType (10/16/08 08:50 PM)
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
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But I have one, and still dont like G2G. I like Bags, LC's and Agar work.
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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SickShroomer
Retired Shroom Master



Registered: 05/25/06
Posts: 361
Loc: Midwest
Last seen: 4 years, 2 days
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Re: Need Advice [Re: dead]
#9088753 - 10/16/08 09:04 PM (16 years, 3 months ago) |
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Quote:
dead said: I'm still using rye flour without a PC with no contams.
Ok, my advice is, NEVER use Rye Flour.. It is possible, but trust me.. This shit turns STICKY!! Its because of the high gluten content, BRF is gluten free!! It will stay nice and airy which is a plus!! Mycelium needs air.. Also, in the times i used rye flour even with a pressure cooker, it contaminated.. Now it could of worked for this guy but since you are just starting, i would suggest going with the simplest way possible..
-------------------- Going to miss the PNW gatherings.
I'm not here trying to make anyone feel stupid or to make myself feel good by putting ppl down. I am just here to help fellow shroomerites in there grows. I do this to keep my own knowledge fresh, and to help everyone succeed!
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T2K_Rusty
Rusty Shackleford


Registered: 10/08/08
Posts: 342
Last seen: 16 years, 1 month
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Re: Need Advice [Re: P.Menace]
#9088896 - 10/16/08 09:28 PM (16 years, 3 months ago) |
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Quote:
P.Menace said: But I have one, and still dont like G2G. I like Bags, LC's and Agar work.
Right on, spawn bags are great. Do not dawg on g2g though. It's probably the best way to get hundreds of jars going. If someone tried to get 200+ cakes like your picture above, they'd have to use 20-30 syringes. That would be extreeeeeeeemly time consuming. With grain to grain & a lil know-how, that can be done in an hour.
-------------------- Sh sh shaw!
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Need Advice [Re: T2K_Rusty]
#9088941 - 10/16/08 09:36 PM (16 years, 3 months ago) |
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Rusty that's why god invented 60CC syringes, but I don't even know if you could do that size of syringe over and over like that. I'd had problems inoculating a set of 12 jars at 4 points for If you were doing 4 points on all 200 that'd be 800 times of flame sterilizing a needle. I doubt a 60cc needle could last 60 squirts you'd have to have multiple needles and alternate. Even then more than a 35cc needle would probibly melt after that. The magnitude of doing that many cakes even at only 1 inoculation point each is mind blowing, all I can ask is why? You could've G2Ged those way the hell quicker.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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T2K_Rusty
Rusty Shackleford


Registered: 10/08/08
Posts: 342
Last seen: 16 years, 1 month
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Quote:
ScavengerType said: oh yea that's right you do, do bags. IMO though G2G requires some alcohol, a paper towel, a smooth metal spoon (perhaps butterknife too in case things get harry), gloves, Tyvek Wrist Sleeves (optional), and a glovebox. I've never had a G2G contaminant with this equipment. I wouldn't call this Super sterile, it's not like you need a flowhood.
Good proceedure, but this can also make newbs paranoid about the whole mycology thing.
Never used a glove box for grain to grain. Only time i've used any sterile material, is rubbing alcohal on a baggie to crumble a colonized cake in, so it can be safely transfered to a few grain jars. I have a *zero contamination rate from grain to grain. Absolutely none. I've done at least 500 grain to grain transfers.
Course this doesn't mean you can breathe everywhere, or do it on a dirty spot. Things have to be kept clean, or failure may take place. The best way to prevent any contamination, aside from a PC, is general cleanlines. Don't do myco work in dirty clothes. Take a shower before you do anything. Do not ever breathe on your work. Don't let the AC or fan run during inoculations. Never let the room get dirty with cobwebs or shit like that. Shiney clean is great. You should be able to eat spaghetti off any surface of the work room, with full confidence. No pets in the work room. No smoking.
-------------------- Sh sh shaw!
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Need Advice [Re: T2K_Rusty]
#9089101 - 10/16/08 10:01 PM (16 years, 3 months ago) |
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glovebox is easier than being a clean freak.
I only eat spaghetti off my floor if I can do it in 5 seconds.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
Edited by ScavengerType (10/16/08 10:02 PM)
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dead
grateful



Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 10 years, 2 months
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Quote:
SickShroomer said:
Ok, my advice is, NEVER use Rye Flour.. It is possible, but trust me.. This shit turns STICKY!! Its because of the high gluten content, BRF is gluten free!! It will stay nice and airy which is a plus!! Mycelium needs air.. Also, in the times i used rye flour even with a pressure cooker, it contaminated.. Now it could of worked for this guy but since you are just starting, i would suggest going with the simplest way possible..
I haven't had a single contamination with rye flour. Yeah it gets sticky, but that can be countered by using a little less water. IME rye cakes also colonize very fast.
-------------------- "The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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