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Offlinealkylbenzene23
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Liquid Culture + Jet dry: Keeping spores distrubted in solution
    #9083222 - 10/15/08 07:34 PM (16 years, 3 months ago)

I'm wondering if anybody has tried putting jet dry in LC's that you plan to grow from multispore injections. This would be a derivative of the keeping spores in solution for spore syringes concept.
I wanted to try this with one of my MS LC's but we didn't have any plain old jet dry.


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Offlinelibertaire
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Re: Liquid Culture + Jet dry: Keeping spores distrubted in solution [Re: alkylbenzene23]
    #9084067 - 10/15/08 09:58 PM (16 years, 3 months ago)

I've heard of it, but the context I heard it in was that the jet dry was added to keep the spores separated.  I don't know what purpose it would serve in an lc.

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Offlinebryanbzl
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Re: Liquid Culture + Jet dry: Keeping spores distrubted in solution [Re: libertaire]
    #9084136 - 10/15/08 10:10 PM (16 years, 3 months ago)

Quote:

libertaire said:
I've heard of it, but the context I heard it in was that the jet dry was added to keep the spores separated.  I don't know what purpose it would serve in an lc.




Your 100% correct. the soup is used to keep the spores from sticking together. Won't do anything for your LC that you'd need to go through the extra exposure to contamination over. Good thought nonetheless.


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conclusion:
poor fucking children of the early 1900's.

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Offlinealkylbenzene23
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Re: Liquid Culture + Jet dry: Keeping spores distrubted in solution [Re: bryanbzl]
    #9085413 - 10/16/08 07:24 AM (16 years, 3 months ago)

Quote:

bryanbzl said:
Quote:

libertaire said:
I've heard of it, but the context I heard it in was that the jet dry was added to keep the spores separated.  I don't know what purpose it would serve in an lc.




Your 100% correct. the soup is used to keep the spores from sticking together. Won't do anything for your LC that you'd need to go through the extra exposure to contamination over. Good thought nonetheless.




Not at all, You put in the jet dry when your making the LC, before sterilization. Also, the spores will be floating around in the LC until they germinate, if they clump together in the syringe they will in the LC.


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