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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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ive got a weird Lc problem, experienced ppl help?
#9082894 - 10/15/08 06:25 PM (16 years, 3 months ago) |
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i have an lc thats about a month old its a karo and water Lc its got alot of mycelium and i know its not contamd, its a big big cloud of it but ive tried innoculating at least 20 pf tek jars and 10 wbs jars and i havent seen any growth at all after 10 days or so id see no growth and id get rid of em and try again. why is my LC not working and not colonizing the substrate?
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 5 years, 14 days
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Re: ive got a weird Lc problem, experienced ppl help? [Re: Demonic_Chronic]
#9082922 - 10/15/08 06:30 PM (16 years, 3 months ago) |
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You know its not contamened because theres a big cloud of white>? mould mycelium is also white and wont sporolate in liquid culture. Just for your information.
I am a litte confused at why you have no colinisation.
Your jars dont smell sour do they?
Some more details on how the grain was prepared sterilised and inoculated. a pic would ne nice too.
With LC you really should see growth in as little as 3 days.
You would be better making a mycelium syringe using a fully colinised grain jar. Growing LC from spores in a suger based liquid is iffy.
You may see success storys here and there. But theres also the contamination forum that people post asking why there lcs looked good and every substrate contamend
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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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dothedew69
Relearning



Registered: 08/27/08
Posts: 624
Last seen: 4 years, 2 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: veda_sticks]
#9082943 - 10/15/08 06:34 PM (16 years, 3 months ago) |
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A big cloud as in a big blob of mycelium? Did you put marbles, broken glass, magnetic stirrer in the LC? Did you break up the mycelium at all by shaking it before you sucked it up with a sterile syringe?
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: ive got a weird Lc problem, experienced ppl help? [Re: dothedew69]
#9083013 - 10/15/08 06:53 PM (16 years, 3 months ago) |
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ok i pced the Pf jars for 60 min at 15 psi and i pcd the grai nfor 70 min at 15 psi and i know what a contamd lc looks like, its definitly mycelium, and the lc was cloned from a stem of a mushroom,
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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dothedew69
Relearning



Registered: 08/27/08
Posts: 624
Last seen: 4 years, 2 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: Demonic_Chronic]
#9083023 - 10/15/08 06:55 PM (16 years, 3 months ago) |
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Did you break the myc up at all when you made the syringe?
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: ive got a weird Lc problem, experienced ppl help? [Re: dothedew69]
#9083037 - 10/15/08 06:59 PM (16 years, 3 months ago) |
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yeah the syringe had alot of myc in it
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: ive got a weird Lc problem, experienced ppl help? [Re: Demonic_Chronic]
#9083050 - 10/15/08 07:02 PM (16 years, 3 months ago) |
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and i ive opened all the jars i tried with no sour smell or ne weird smell and no sight of contam, they smelled and looked fine but with no growth
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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alkylbenzene23
Indeed



Registered: 08/16/08
Posts: 615
Last seen: 15 years, 1 month
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Re: ive got a weird Lc problem, experienced ppl help? [Re: Demonic_Chronic]
#9083127 - 10/15/08 07:17 PM (16 years, 3 months ago) |
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Did you let the jars cool before injecting?
-------------------- If you try to lick your cat while tripping, and they bite you, are you even?
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Nuzzle
Misunderstood



Registered: 09/29/08
Posts: 840
Last seen: 15 years, 1 month
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Re: ive got a weird Lc problem, experienced ppl help? [Re: alkylbenzene23]
#9083154 - 10/15/08 07:22 PM (16 years, 3 months ago) |
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I am assuming you did all this in a sterile environment? creating the lc, transfering the lc >> syringe, and innoc?
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**If you have any spare spore prints lying around, and your feeling 'giving' drop me a PM**
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: ive got a weird Lc problem, experienced ppl help? [Re: Nuzzle]
#9083480 - 10/15/08 08:29 PM (16 years, 3 months ago) |
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ya everything was smooth and sterile im gunna try 1 more batch of pf
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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gofudgeyourself
Student



Registered: 09/01/08
Posts: 710
Loc:
Last seen: 13 years, 7 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: Demonic_Chronic]
#9083760 - 10/15/08 09:06 PM (16 years, 3 months ago) |
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sounds really stupid but are you SURE youre breaking up the mycelium and when you suck it up in the syringe youre actually getting mycelium inside? it sounds retarded but ive done it 2 or 3 times before realizing it.
are you sure its mycelium? lol
i have no idea, this is why i dont enjoy LC, something goes wrong and it could be 1000001 different things.
-------------------- "believe it if you need it, or leave it if you dare."
"turn all the hate in the world, into a mocking bird. make it fly away"
Edited by gofudgeyourself (10/15/08 09:07 PM)
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bryanbzl
Spawn Runner



Registered: 03/11/07
Posts: 563
Last seen: 6 years, 9 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: gofudgeyourself]
#9083890 - 10/15/08 09:28 PM (16 years, 3 months ago) |
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hate to be the one to shit on this parade. BUT... You either have a contaminated LC or your jars aren't prepared right. you don't need to have a sour smell to have a contamination. even a smell similar to that of honey (kinda fruity notes) can be a bacterial contamination, along with many other smells. Another possibility is that your LC is in some sort of a recovery state. I've inoculated jars with LC that have taken just about 2 weeks to see any signs on colonization. If you have any spore syringes left, try knocking up your next batch with your LC and some spores. make some new LC while your at it.
good luck with everything dude.
-------------------- Cheers,
bzl
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."
conclusion:
poor fucking children of the early 1900's.
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libertaire
liberator



Registered: 08/06/08
Posts: 4,204
Last seen: 4 years, 6 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: bryanbzl]
#9083932 - 10/15/08 09:35 PM (16 years, 3 months ago) |
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Pictures would help solve this mystery also.
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bryanbzl
Spawn Runner



Registered: 03/11/07
Posts: 563
Last seen: 6 years, 9 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: libertaire]
#9083956 - 10/15/08 09:39 PM (16 years, 3 months ago) |
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Quote:
libertaire said: Pictures would help solve this mystery also.
pictures won't tell much either, but they're better than no pictures that's for sure. I've had cloudy LC's work fine and that can be an indicator for contamination. your only way to find out if an LC is viable is to test it and in your case, your going to need a control... such as a spore syringe vs. your LC on the same prep jars.
-------------------- Cheers,
bzl
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."
conclusion:
poor fucking children of the early 1900's.
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JMR
Deviator



Registered: 03/12/08
Posts: 655
Loc: Zion Highlands
Last seen: 1 year, 10 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: bryanbzl]
#9084083 - 10/15/08 10:01 PM (16 years, 3 months ago) |
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Your jars could possibly be to dry! You never said anything about the moisture contents of your Jars.
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: ive got a weird Lc problem, experienced ppl help? [Re: JMR]
#9084346 - 10/15/08 10:47 PM (16 years, 3 months ago) |
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Contaminated LC looks almost like good LC.. The only way to tell the two apart most of the times it to test it on some jars.. It took one of my LC's 6 days to show growth.. So you can try and wait it out..
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