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InvisibleCDS
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What is this little white dot stuff?
    #9080399 - 10/15/08 09:38 AM (16 years, 3 months ago)



It appeared in the little divot I cut in the agar to keep the spore solution from sliding all over the place. It would seem that my circular loop tool I used to cut them was dirty, strange because I flamed and wiped it with alcohol, hmmm...

Edited by CDS (12/21/08 01:26 AM)

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OfflineRogerRabbitM
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Re: What is this little white dot stuff? [Re: CDS]
    #9080415 - 10/15/08 09:41 AM (16 years, 3 months ago)

Bacteria.  Next time, flame the inoculating loop, but don't ever wipe with alcohol after flaming.  Flame the loop red hot, cool it in the petri dish, and then swipe a few spores onto the agar from your print.

You can easily transfer mycelium away from the bacteria to a new dish.  Repeat in a few days until you have a clean culture.
RR


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InvisibleCDS
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Re: What is this little white dot stuff? [Re: RogerRabbit]
    #9080820 - 10/15/08 11:06 AM (16 years, 3 months ago)

If bacteria can survive alcohol, should I be worried about that when I am sanitizing my gloves w/alcohol? Is there anything that kills everything?

-Thanks

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OfflineRogerRabbitM
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Re: What is this little white dot stuff? [Re: CDS]
    #9082007 - 10/15/08 03:14 PM (16 years, 3 months ago)

Flame kills everything.  Nothing that I know of can survive on red hot steel.

Your gloves are fine cleaned with alcohol.  You don't directly handle the mycelium with your gloves.  Just don't get your alcohol soaked gloves too close to the flame.
RR


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