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OfflineSpongiform
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Couple Questions
    #9078334 - 10/14/08 08:39 PM (16 years, 3 months ago)

Im setting up an aquarium in preperation for a future batch, so I have a few questions to make sure Im on the right track.

Incubation:
I have a 5 gallon bucket that sits inside another 5 gallon bucket partially filled with water that has a aquarium heater submerged.  Takes a few days of tweaking but you can get the temperature stable to within 1-2 degrees.  My understanding is that the optimal incubation temperature is 86F - is this correct?

Fruiting:
Im setting up an aquarium (10 gallon) to use for fruiting. I have a 1 gallon fruit juice plastic jug (sturdy) halfway filled with volcanic pebbles and water.  A fish tank bubbler sends air in the bottom then a second tube leads out (about 1 inch above the water line) to the aquarium.  The lid isnt air tight but pretty close.  Ive had it running for a few days and its maintained a steady 90% relative humidity.

Will I need to set up some sort of exhaust system? I have fresh air coming in 24/7 but my understanding is that the co2 is heavy and therefore will sink to the bottom.

I plan to keep the fruiting chamber at 76F using a heating pad + external heater.  If that doesnt do the trick I can move the whole setup into a small unused bathroom and just heat the whole room.

So to recap:
1: is 86F optimal for incubation
2: is 76F + 90% humidity optimal for fruiting
3: Will I need extra exhaust or will the fresh air supply be sufficient?
4: any other tips/advice? ;-D

(ps: planning on doing casings)

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OfflineCascadian
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Re: Couple Questions [Re: Spongiform]
    #9078470 - 10/14/08 09:04 PM (16 years, 3 months ago)

Quote:

My understanding is that the optimal incubation temperature is 86F - is this correct?




You are correct that you want your mycelium kept at that temperature for fastest colonization, but keep in mind that mycelium creates heat of its own.  If your incubator is at 86, your mycelium is probably above 90, and growth slows exponentially above 86.  Your safest bet would be to keep it somewhere between 75-80 and let the natural heat from the mycelium heat itself.

Quote:

2: is 76F + 90% humidity optimal for fruiting




Again, 76 includes the heat of the mycelium.  While they aren't putting out as much heat while they are fruiting as when they are colonizing, it's still something to account for.  Keep your FC between 70-75.  Also, you should shoot for a RH above 95% if you can get it.  I'm sure 90% will be fine, but that extra 5% will keep them extra happy.  Misting a few times a day will probably help your humidifier.

Quote:

3: Will I need extra exhaust or will the fresh air supply be sufficient?




You should definitely have lots of holes in your aquarium for more Fresh Air Exchange.  Get the CO2 out and more fresh air in.  Your fresh air system sounds pretty sweet, but I think you should still have a lot more fresh air coming in than what an aquarium pump can provide, but thats just my opinion.

Good luck!

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OfflineNuzzle
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Re: Couple Questions [Re: Cascadian]
    #9078495 - 10/14/08 09:08 PM (16 years, 3 months ago)

I had a stalled growth when my incubator got over around 83-84F... I had best results and faster growing between 78-82 ish.... The more holes the better... if it is in an open room, then that should be enough, maybe fan it out once or twice a day...


--------------------
:hi:**If you have any spare spore prints lying around, and your feeling 'giving' drop me a PM** :dancer:

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OfflineP.Menace
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Re: Couple Questions [Re: Nuzzle]
    #9078513 - 10/14/08 09:11 PM (16 years, 3 months ago)

People are beginning to see the mis informational part of the temperature scheme now I see.
forget the incubator. 70-80 is fine and even best IMO, look at it this way, if your comfortable in your house, then so are your mushrooms.


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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OfflineSpongiform
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Re: Couple Questions [Re: P.Menace]
    #9078904 - 10/14/08 10:17 PM (16 years, 3 months ago)

I doubt they like it as cold as I do, I'm a 55-60f kinda guy. 
I'll aim for  80f for incubation and 72f for fruiting then. 

My aquarium is glass so I can't really drill a hole in the side of it. 
I could however run an air line in and stick it in the bottom and have it on a timer.  I also plan on keeping the casings a few inches off the bottom since the co2 sinks. 

I need to keep the system as enclosed as possible, it'll be REALLY dry in here by the time I'm ready to fruit (20-30% RH) and I don't turn on the heat unless it's below 40f (except the bathrooms).

Thanks for all the advice - and would pictures of my setup be helpful?

Edit; Here's a diagram


Edited by Spongiform (10/14/08 10:39 PM)

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OfflineCascadian
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Re: Couple Questions [Re: Spongiform]
    #9079279 - 10/14/08 11:36 PM (16 years, 3 months ago)

That diagram is just too cute.

Could you maybe poke a few holes in your aquarium lid?  If you put some wet perlite on the bottom and mist 3x a day you wont have to worry about your humidity levels, and your FAE is going to make your goomies really really happy.

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OfflineSpongiform
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Re: Couple Questions [Re: Cascadian]
    #9079611 - 10/15/08 01:22 AM (16 years, 3 months ago)

I'm building a new lid soon.  Buying a piece of plexiglass and drilling holes for the air exchanges.  I'm trying to avoid having anything that requires daily maintenance since I like to do alot of camping/hiking/fishing etc. so I can't be around to watch it every single day. 

Do you think having the fresh humid air running 24/7 + the co2 removal /2x day will be sufficient?


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.

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Offlineveda_sticks
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Re: Couple Questions [Re: Spongiform]
    #9079781 - 10/15/08 03:48 AM (16 years, 3 months ago)

Id suggest making a shotgun fruiting chamber. Using an air pump wont provide enough air exchange. You will still have to fan out your terrarium quite a bit. Manual air  exchanges work better than an air pump (even an air pump that pumps plenty of air in).

Your house is pretty cold, so it may be helpfull to incubate. Or is there somewhere that is kept a little warmer? dont incubate any higher than 81F. The 86F figure has been discredited years ago. People see a graph with paul stamets name on it and take it as fact.

AT above 83F myceial growth falls sharply while stimulating thermophilic moulds and bacteria. Remember that colinising mycelium within a substrate will produce its own heat as it colinises.

for pf tek, incubate no higher than 81F, the larger the substrate, the lower you will need to incubate.

Room temperature (75F) is fine, between that and 80F the difference in growth is minimal if anything.

For humidification your will want perlite. Check out the shotgun fc link in my signiture.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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