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OfflineDamion5050
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First Petri Dish Attempt
    #9077429 - 10/14/08 06:02 PM (16 years, 3 months ago)

Well here are some pic of my first attempt at agar.. I knocked them up and checked them today, and I see like 7 or 8 little spots of white..  When looking at them close up they are not really rope like they are like a little spot of cotton, they are fluffy..  So what do you think, go or not good ??




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InvisibleFooMan
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9077561 - 10/14/08 06:28 PM (16 years, 3 months ago)

Good. :thumbup:


--------------------

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Invisibleimnotthere
Registered: 02/23/08
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9077571 - 10/14/08 06:30 PM (16 years, 3 months ago)

i can't stop looking at your avatar, and wondering what she is saying....  you have a full size version perhaps?

as for the spots, my first ones currently look just like yours here.  I think it is mold.  I think mine are mold...

did you inoculate with spore syringe?

next time I'm gonna take a single colonized grain and inoculate with that... my solution just seemed like it ran all over the agar, which I suspect yours did too.

a single colonized grain should make isolating rhizo easier too I beleive... and it won't run all over the place, it will stay in the center.


Lets wait for some knowledgable folks to answer tho!

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Offlineveda_sticks
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Re: First Petri Dish Attempt [Re: FooMan]
    #9077578 - 10/14/08 06:32 PM (16 years, 3 months ago)

www.mushroomvideos.com

theres a good video on strain isolation


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleFooMan
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9077580 - 10/14/08 06:32 PM (16 years, 3 months ago)

Quote:

imnotthere said:
i can't stop looking at your avatar, and wondering what she is saying.... 




Do you really care what she's saying? :lol:


--------------------

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OfflineScavengerType
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Re: First Petri Dish Attempt [Re: FooMan]
    #9077632 - 10/14/08 06:40 PM (16 years, 3 months ago)

actually it's been bugging me too. And while your here fooman why'd you change your avatar from the mushroom that kinda looked like a tit?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Invisibleimnotthere
Registered: 02/23/08
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Re: First Petri Dish Attempt [Re: FooMan]
    #9077656 - 10/14/08 06:45 PM (16 years, 3 months ago)

in my mind she is saying dirty dirty things - so yes I CARE! lol


don't listen to my advice, if these folks say good its good.  Upon checking mine again my second generation looks more rhizo... so I think the cotton is just 100s or thousands of substrains right on top of each other, giving the appearance of cotton...

question here, don't mean to jack but this is useful...

How do you know when it is mold?  I've heard agar can get mold that is white and cottony.  Does it just take time to tell mold vs mycelium?

I just got the RR video today in the friggin mail!!!  I got it almost exclusively for the agar section...

AGAIN - THIS IS THE PERV IN ME TALKING :  DO YOU HAVE THAT AVATAR FULL SIZE?  THIS IS CRITICAL MAN.  She looks like a lovely lady I once stoinked.

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InvisibleFooMan
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Re: First Petri Dish Attempt [Re: ScavengerType]
    #9077687 - 10/14/08 06:49 PM (16 years, 3 months ago)

I got tired of the avatar. The nipple cap avatar was from the fruit at the bottom right corner of this pic:



I'm guessing the chick is singing the song she's dancing to or saying Foo Man, Foo Man, Foo Man. :shrug: :lol: I'm usually not looking at her mouth.


--------------------

Quick WBS Prep

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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: FooMan]
    #9077942 - 10/14/08 07:35 PM (16 years, 3 months ago)

lol the only time I look at her mouth is when my dick is in it..  I am not isolating a strain per say, well not yet..  The is just some myco scrapped off of a corn kernel from a g2g jar I had..  But when I get something I decide I like I will isolate it..  Just playing around with agar to try and get the hand of it..

I don't have a blown up picture of the girl but I will try and find one..

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Re: First Petri Dish Attempt [Re: Damion5050]
    #9077985 - 10/14/08 07:44 PM (16 years, 3 months ago)

I always thought she was saying
"Put it on Bitch"
but thats just me probably.
I like to think I can somewhat accurately read lips, but sometimes that just becomes Me eavesdropping incorrectly:lol:

Those trays look good, but you'll want to begin isolating away from any contams as soon as you see growth.
Isolate from the farthest away side from the contams, and pick the most aggressive rhizomes:thumbup:


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: P.Menace]
    #9078021 - 10/14/08 07:51 PM (16 years, 3 months ago)


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OfflineP.Menace
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9078025 - 10/14/08 07:52 PM (16 years, 3 months ago)

yeah... definitely Put it on Bitch!

ha ha your avatar and the big pic move at slightly different speeds


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: P.Menace]
    #9078029 - 10/14/08 07:53 PM (16 years, 3 months ago)


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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9078052 - 10/14/08 07:56 PM (16 years, 3 months ago)


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Invisiblemusher_420
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9078069 - 10/14/08 07:58 PM (16 years, 3 months ago)

Well ah.. The thing about agar work.. ah... you see.. Umm uhh

Why are watching those so damn addictive!


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: musher_420]
    #9078112 - 10/14/08 08:05 PM (16 years, 3 months ago)

LOL yes they are very nice..  :whacker:

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Invisibleimnotthere
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9078272 - 10/14/08 08:28 PM (16 years, 3 months ago)

for my first attempt at agar I'm using sporeworks pre-made MAE.  do you guys use this, make your own, or get the pre-mixed powder? 

I'm going to switch to the powder and make mine in a long neck whickey like bottle to be easier to pour...

how much do you think the pound of mae will make?  its like less than $40 from several of our vendors.  Seems to me this is a shit ton of agar is it not?

AND...

does that GIF get any bigger der Damion5050?

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InvisibleFooMan
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078313 - 10/14/08 08:34 PM (16 years, 3 months ago)

Quote:

imnotthere said:
for my first attempt at agar I'm using sporeworks pre-made MAE.  do you guys use this, make your own, or get the pre-mixed powder? 

I'm going to switch to the powder and make mine in a long neck whickey like bottle to be easier to pour...

how much do you think the pound of mae will make?  its like less than $40 from several of our vendors.  Seems to me this is a shit ton of agar is it not?




I think you mean MEA.

Edit: I thought you bought premixed MEA and were going to buy 1 pound of agar agar powder to make your own agar mix later. 1 pound of premixed MEA and one pound of agar agar are 2 totally different things. My bad. I should have read instead of skimming through.

Quote:



AND...

does that GIF get any bigger der Damion5050?




Dude, it doesn't matter how big he makes it or how low you duck your head down below the monitor..... You still won't be able to see her nipples.

Edited by FooMan (10/14/08 08:40 PM)

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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078316 - 10/14/08 08:35 PM (16 years, 3 months ago)

the pound will make...
40g to 1 L water, so... over 12 L of media
and a L of media will fill some 50 dishes.
so 50x12=600 dishes.

and IME using more like 30g per L of water works better.
I have had too many times where I PC the premade MEA with 40g pre L, and it never sets up due to over heating I think... not sure tho


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Invisiblemusher_420
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078321 - 10/14/08 08:36 PM (16 years, 3 months ago)

A pound of MEA powder should make over 11 liters of media and a liter easily makes 50 plates. So you could make over 550 plates with that.

It's 40g per Liter BTW.

You could just buy agar agar powder and malt extract from a brewers supply.  You could also get dextrose from the brewers supply and make PDA.  It's good to switch up agar recipes when storing a culture long term.  I believe it's common practice to switch up the media used every 6 months.

EDIT
Looks like I'm tooo slow haha...


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

Edited by musher_420 (10/14/08 08:38 PM)

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Offlinedothedew69
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078333 - 10/14/08 08:38 PM (16 years, 3 months ago)

im sorry but please who is that I need her name! She is just so damn gorgeous!

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OfflineP.Menace
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Re: First Petri Dish Attempt [Re: P.Menace]
    #9078351 - 10/14/08 08:42 PM (16 years, 3 months ago)

Quote:

musher_420 said:
A pound of MEA powder should make over 11 liters of media and a liter easily makes 50 plates. So you could make over 550 plates with that.

It's 40g per Liter BTW.

You could just buy agar agar powder and malt extract from a brewers supply.  You could also get dextrose from the brewers supply and make PDA.  It's good to switch up agar recipes when storing a culture long term.  I believe it's common practice to switch up the media used every 6 months.

EDIT
Looks like I'm tooo slow haha...




Quote:

P.Menace said:
the pound will make...
40g to 1 L water, so... over 12 L of media
and a L of media will fill some 50 dishes.
so 50x12=600 dishes.

and IME using more like 30g per L of water works better.
I have had too many times where I PC the premade MEA with 40g pre L, and it never sets up due to over heating I think... not sure tho




Like synchronized swimming.


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Invisibleimnotthere
Registered: 02/23/08
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Re: First Petri Dish Attempt [Re: musher_420]
    #9078441 - 10/14/08 08:59 PM (16 years, 3 months ago)

mycosupply has 500 grams MEA for less than $40 and that is over a pound

http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=MEAR01

I've heard as well to mix it light on nutrients - makes the mycelium stretch out in search of nutes.

that much agar excites me in ways that she never could.

Why do folks here make their own agar when this stuff is so cheap?  Is their a benefit to making your own?

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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078482 - 10/14/08 09:06 PM (16 years, 3 months ago)

because 1/2 a lb of regular agar agar is far superior and costs less to mix up yourself, seeing as how you only need about 10g per L of water to make nonnutritious agar


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Invisibleimnotthere
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Re: First Petri Dish Attempt [Re: P.Menace]
    #9078504 - 10/14/08 09:09 PM (16 years, 3 months ago)

not familiar with that although I have seen it.  Is Agar Agar different than MEA?  What is nonnutrious agar? 

where do you get agar agar?

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OfflineScavengerType
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078545 - 10/14/08 09:17 PM (16 years, 3 months ago)

to above poster
Quote:

You could just buy agar agar powder and malt extract from a brewers supply.  You could also get dextrose from the brewers supply and make PDA.



I think agar is agar powder and MEA is malt extract with agar powder and PDA potato dextrose /w agar powder. I'm pretty sure agar agar is non-nutritious form and the additive is the nutes.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Invisibleimnotthere
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Re: First Petri Dish Attempt [Re: ScavengerType]
    #9078561 - 10/14/08 09:21 PM (16 years, 3 months ago)

that makes more sense to me as well.  It may be cheaper to add your own nutes, but conveince and accuracy of the pre-mixed just seems to be worth it even when price shopping like I do.

just my thought and opinion

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Invisiblemusher_420
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078569 - 10/14/08 09:22 PM (16 years, 3 months ago)

Mycosupply has regular agar too.
http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=AGAR01
I've seen other places having it cheaper online and some Asian food stores may have it as well but I was never lucky finding it.

Like I said before people buy agar powder so they can vary the recipes for one and because the other additives like light malt extract, dextrose and potatoes are very cheap.  You buy premixed MEA and your mostly paying for light malt extract which actually costs a couple bucks a brewers supply.

I've heard food grade agar doesn't have the gel strenth to make a good media but the food grade stuff I picked up has been fine for me.


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

Edited by musher_420 (10/14/08 09:25 PM)

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Invisibleimnotthere
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Re: First Petri Dish Attempt [Re: musher_420]
    #9078595 - 10/14/08 09:28 PM (16 years, 3 months ago)

interesting.  As a well equipped agar nooooob, what do you suggest I get.  I plan on working agar on several strains including edibles, and plan to maintain some isolates long term eventually.

So you have to add the nutrients to the regular agar in the link there?  still unclear on which to get

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Invisiblemusher_420
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9078624 - 10/14/08 09:35 PM (16 years, 3 months ago)

Yeah you'll need to add other ingredients to the regular agar powder.

Here is the recipes
http://www.shroomery.org/9393/FastFreds-Media-Cookbook

It's up to you if you want pre-mixed or not.  If you have a digital scale it should be no problem making any of the mixtures yourself and you'll have way more options if you go with the agar powder to mix yourself.

BTW you totally hijacked this thread lol but we'll let that go cause we are nice to nooobs here lol but try to start your own next time.


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

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Invisibleimnotthere
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Re: First Petri Dish Attempt [Re: musher_420]
    #9078658 - 10/14/08 09:40 PM (16 years, 3 months ago)

yeah but the thread was very much dead, thats why it was rightfully jacked by me and the asian princess.

Edited by imnotthere (10/14/08 09:41 PM)

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Re: First Petri Dish Attempt [Re: musher_420]
    #9078671 - 10/14/08 09:41 PM (16 years, 3 months ago)

well if the thread jacking has already commenced can you tell me how you go about finding agar in the grocery store, I've never seen it how do you find it?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: ScavengerType]
    #9079135 - 10/14/08 11:05 PM (16 years, 3 months ago)

Quote:

ScavengerType said:
well if the thread jacking has already commenced can you tell me how you go about finding agar in the grocery store, I've never seen it how do you find it?




Try health food stores or I can give you a link if you want to purchase agar powder online then go to the store and purchase instant boxed potatoes and mix them together and make yourself some PDA

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Invisiblebarnett256
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Re: First Petri Dish Attempt [Re: imnotthere]
    #9079266 - 10/14/08 11:33 PM (16 years, 3 months ago)

Quote:

imnotthere said:
... my solution just seemed like it ran all over the agar, which I suspect yours did too.




I have no experience with mycology or agar, but perhaps you could cut a chunk of agar out; making a reservior . ..?.

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Re: First Petri Dish Attempt [Re: barnett256]
    #9079428 - 10/15/08 12:09 AM (16 years, 3 months ago)

good idea barnet I'll remember that one.

as for damion Thanks but I cut up my CC a while ago so I can't buy online till I get a new one. I'll check out the HF stores. Instant boxed potatos + agar = PDA? that'd be easy where'd you read that?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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OfflineDamion5050
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Re: First Petri Dish Attempt [Re: ScavengerType]
    #9079460 - 10/15/08 12:20 AM (16 years, 3 months ago)

Hey it's me again I heard that from a tek it's called the grocery store agar tek.. http://www.shroomery.org/9427/Grocery-Store-Agar-Tek ; Just take agar and instant potatoe flakes and mix them together per the recipe and it creates PDA..  You want to mix 1/4 cup agar powder with 2 tablespoons of instant potatoe flakes..

Also here is a link to some agar powder just bookmark it or what not.. It seems expensive but in the long run it will save you money.. http://www.amazon.com/Agar-Powder-NOW-Foods-lbs/dp/B000JN8MT4/ref=sr_1_1?ie=UTF8&s=hpc&qid=1224051555&sr=8-1

I got some pre mixed MEA from mycosupply, but next time when I got the money I'll be going for the home made..

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Invisiblemusher_420
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9079845 - 10/15/08 04:53 AM (16 years, 3 months ago)

PDA is potato - dextrose - agar.

Dextrose is like core sugar and important to the mix.  Check out this link for most recipes you'll need.  They are very strait forward.

http://www.shroomery.org/9393/FastFreds-Media-Cookbook

You'll notice he used honey in that other post to replace the dextrose. 


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OfflineJuke Adro
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Re: First Petri Dish Attempt [Re: Damion5050]
    #9079852 - 10/15/08 05:00 AM (16 years, 3 months ago)

I know I'm a little late but I can see some bad colonies in that dish, the ones near the edges look iffy.


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OfflineScavengerType
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Re: First Petri Dish Attempt [Re: Juke Adro]
    #9088557 - 10/16/08 08:29 PM (16 years, 3 months ago)

It says to add 4.5grams or nearly a quarter cup of ground agar agar. However I've out and bought the powdered form of agar. Would I add 4.5 grams of that with that recipe Damion posted or what's the deal?


edit: Never mind I found it, didn't properly search it, they are the same per weight BTW if anyone need know.


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Edited by ScavengerType (10/16/08 08:51 PM)

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