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atrilis
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Registered: 10/12/08
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First Spore Print!
#9077012 - 10/14/08 04:52 PM (15 years, 5 months ago) |
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Heya, I recently came across some psilocybe cubensis "gulf coast" shrooms and i made my first print as an attempt to grow. I was wondering if this pring is exceptional for use. I sterilized the aluminum foil at 400 Degrees F and the bag its in with 91% iso alcohol. After the picture i folded the aluminum foil in half and fold in the edges to seal it off in the bag.
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XxShibbyxX
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Re: First Spore Print! [Re: atrilis]
#9077060 - 10/14/08 05:04 PM (15 years, 5 months ago) |
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I may be missing something but did you say you sterilized the foil with the print on it? The print looks good. Its how I did my first grow ( which Im still on by the way )
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kramedit
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Re: First Spore Print! [Re: atrilis]
#9077062 - 10/14/08 05:04 PM (15 years, 5 months ago) |
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looks good nice job I'm sure it'll bring lots of joy under the microscope
-------------------- ***WARNING*** I'm a pathological liar. Only God can create something of value out of nothing. I've never been through the desert on a horse with no name but I've walked in the sand with a horse named tony. It always feels good to be out of the rain. Every fly has a buzz.
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atrilis
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Re: First Spore Print! [Re: kramedit]
#9077076 - 10/14/08 05:06 PM (15 years, 5 months ago) |
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lol no i didn't sterilize it with the print on it. i sterilized it before hand and the bag then i printed it over night and stuck it in the bag open took the pick then quickly sealed off the foil in the bag.
a print like that...about how many 18G 10cc syringes do you think it will make? 2?
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veda_sticks
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Re: First Spore Print! [Re: atrilis]
#9077085 - 10/14/08 05:09 PM (15 years, 5 months ago) |
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Please post your pictures to your gallery on the shroomery. I wont click that link.
Is that a wild print?
Keep in mind that a mushroom in the wild could be heavily infiltrated by contaminants. Its highly recommended that prints from the wild be grown out on agar as with that method its possible to transfer away form contaminants untill you have a clean culture.
Some cultivators will even recomend growing out on agar wether its a wild print or indoor print as spore prints are not sterile.
Theres no harm in trying, but from a wild print theres some risk. Be sure to follow a good spore print to syringe tek and be sure to use a glovebox.
You dont need to use much spores. A dark syringe is a bad idea. Because prints are not sterile, the more spores you use, the more chance contaminants get picked up.
Also heavily loaded spore syringes have never been proven to perform well during fruiting due to massive amounts of ginetics growing.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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XxShibbyxX
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Re: First Spore Print! [Re: atrilis]
#9077091 - 10/14/08 05:10 PM (15 years, 5 months ago) |
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XxShibbyxX
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Re: First Spore Print! [Re: XxShibbyxX]
#9077101 - 10/14/08 05:12 PM (15 years, 5 months ago) |
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atrilis
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it's a wild print...i'm going to grow it in a glove box but i'm going to look out for tricoderma or whatever it's called. i figure it's worth a shot and if it doesn't work i can always buy a spore print from a vender. but anyways i would rather experiment on a wild print that's free and fail on my first grow due to lack of experience rather than ruining a spore print that i bought. practice makes perfect . If it does get contaminated i'll throw it in some verm and black cow manure in the yard and see what nature's got stored
Also, how many syringes should i make with that print? it's pretty dark as far as i can tell.
thanks for your help and i appreciate your time!
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XxShibbyxX
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Posts: 260
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Re: First Spore Print! [Re: atrilis]
#9077126 - 10/14/08 05:17 PM (15 years, 5 months ago) |
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I would think at least 10 syringes, think of it as a pie and cut ten pieces...there are ALOT of spores in each of those 10 sections...should be plenty to work
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veda_sticks
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Re: First Spore Print! [Re: atrilis]
#9077265 - 10/14/08 05:41 PM (15 years, 5 months ago) |
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Quote:
atrilis said: it's a wild print...i'm going to grow it in a glove box but i'm going to look out for tricoderma or whatever it's called. i figure it's worth a shot and if it doesn't work i can always buy a spore print from a vender. but anyways i would rather experiment on a wild print that's free and fail on my first grow due to lack of experience rather than ruining a spore print that i bought. practice makes perfect . If it does get contaminated i'll throw it in some verm and black cow manure in the yard and see what nature's got stored
Also, how many syringes should i make with that print? it's pretty dark as far as i can tell.
thanks for your help and i appreciate your time!
You certainly have nothing to loose, except a little substrate.
I wouldnt recomend LC. Especially to use with a wild print. You dont know that you have a clean LC until you inocu some substrate, since moulds dont sporolate in liquid culture. All mould mycelium is white, so you can see why you cant tell if its good or not
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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XxShibbyxX
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Yea very true veda. If he is gonna do grain and wants to get as many syringes as possible off the print he could try agars Grain LC method so he could tell if there are any contams first though.
Here it is... http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6817701/an/0/page/0
just a thought though. Since it seemed like he wanted to get as much out of the print as possible.
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veda_sticks
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Re: First Spore Print! [Re: XxShibbyxX]
#9077545 - 10/14/08 06:24 PM (15 years, 5 months ago) |
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Ill need to put that in my signiture. Id recommend that method over lc from spores any day
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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XxShibbyxX
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Loc: California, USA
Last seen: 12 years, 11 months
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Yup! If my damn jars werent brf Id have lots of LC here in about a week lol
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atrilis
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Re: First Spore Print! [Re: XxShibbyxX]
#9077587 - 10/14/08 06:33 PM (15 years, 5 months ago) |
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i'm getting an access denied on that agar's LC tek....can you copy paste it to this actual thread? thanks for the help
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XxShibbyxX
Adventurer
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Posts: 260
Loc: California, USA
Last seen: 12 years, 11 months
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Re: First Spore Print! [Re: atrilis]
#9077658 - 10/14/08 06:45 PM (15 years, 5 months ago) |
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It has pictures and stuff so I probably cant on here. Do a search for Grain LC from agar. Or keep trying that link.
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veda_sticks
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Re: First Spore Print! [Re: atrilis]
#9077664 - 10/14/08 06:47 PM (15 years, 5 months ago) |
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Pint jar on right is multispore inoculated (SA). Allowed to colonize near 100%. Syringe is 60 ML w/18 gauge needle. Quart jar on left is sterilized water.
Aspirated 60 ML sterile water into syringe.
Injected 60 ML sterile water into colonized grain jar.
Shook grain jar to liberate mycelium from grain.
Injected total of 180 ML sterile water into grain jar. (shaking mildly between each injection)
Aspirated out mycelium laden water from grain jar. (all I could aspirate out of the grain jar was 150 ML) Then, injected mycelium laden water back into sterile water jar.
Approximately 500 ML of Mycelium laden water. (enough LC for approximately 50....10 ML syringes)
The advantage of this procedure is that you can be assured. The culture in clean. Because you can VISUALLY inspect colonization in the grain jar. (if there is FUNK, you can see it )
That is not the case, with sugar type LC's. (multispore syringe inoculated) They can be contaminated, from day 1. And, you NEVER know it. Until you inoculate spawn jars with that LC. Then, have every single spawn jar GO SOUTH.
--------------------
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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atrilis
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Re: First Spore Print! [Re: atrilis]
#9077695 - 10/14/08 06:50 PM (15 years, 5 months ago) |
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okay a question....it says to inject 2 cc's of spore solution into the jar...then a few steps later it says to scrape a spore print and get as many spores as you can on the tip of the knife and put in there...what is the reason for putting spore solution AND spores in it?
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atrilis
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Re: First Spore Print! [Re: atrilis]
#9077705 - 10/14/08 06:52 PM (15 years, 5 months ago) |
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Thanks for the help i really appreciate...but i have another question...what can the contams do to you? make you a little sick? or can it be bad?
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XxShibbyxX
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Re: First Spore Print! [Re: atrilis]
#9077716 - 10/14/08 06:55 PM (15 years, 5 months ago) |
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Agar is showing 2 different methods. One is if you already have a syringe of spores and the other is if you just want to do it with a print. Contams are bad. Im not sure exactly what they do to you but I sure getting very sick is one of them. Ill let someone else answer that part for you because Im not to sure.
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atrilis
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Re: First Spore Print! [Re: atrilis]
#9077760 - 10/14/08 07:04 PM (15 years, 5 months ago) |
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okay that definately makes more since now. so after i make this LC and add the spores....when i withdrawl the inoculation will it matter if any of the liquid that's not inoculation comes into the syringe with it?
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