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MHbound
Ballin Out At All Cost
Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 11 months
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Re: jars wont colonize anymore [Re: lipa]
#9076550 - 10/14/08 03:05 PM (15 years, 5 months ago) |
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Stick them above the fridge...It's a nice warm spot without using an incubator if you live in cold climate.
Also, it sounds like your syringe may be bad...No growth at all??? In 0 jars...I would get some new syringes.
Pressure cook your jars for 90 minutes, and if you still don't get any growth then your syringes aren't right.
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Re: jars wont colonize anymore [Re: sumo]
#9076611 - 10/14/08 03:20 PM (15 years, 5 months ago) |
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So the PF tek at http://www.shroomery.org/8409/PF-Tek says: Maximum fruiting formula: 1/4 cup of brown rice powder 1/2 cup of vermiculite 60 cc's water
That is the same as 1 part BRF , 1 part water , and 2 part Verm. Is that correct?? Is my math wrong?
Why do I need a PC?? I never used it the first few times and I had great results. Unfortunatly a PC is not in the near future. I use a tall stainless steel pot. The jars are elevated out of the water. i steam them for 1.5 hours. If not using a PC was the problem, then I would get contaminates, right? I dont get anything to grow. I cant even get a contaminate to grow, that is another reason I thought I might be over sterilizing.
I do have jars over the fridge also in addition to the closet. Not enough room in any one area for 5 cases. I dont want to stack the cases because I am worried about gas exchange.
How long should I give the jars to show the START of myc growth?? On my succesfull atttempts , I always saw growth in 2 to 4 days. So if I wait 2 weeks and see NO growth then its not going to do anything ,right?
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seven
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Registered: 06/09/08
Posts: 1,478
Loc: north carolina
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Re: jars wont colonize anymore [Re: sumo]
#9076634 - 10/14/08 03:27 PM (15 years, 5 months ago) |
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give em two weeks.
-------------------- grind
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seven
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Registered: 06/09/08
Posts: 1,478
Loc: north carolina
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Re: jars wont colonize anymore [Re: sumo]
#9076641 - 10/14/08 03:28 PM (15 years, 5 months ago) |
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give em two weeks. if nothing happens most people start over.
-------------------- grind
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Re: jars wont colonize anymore [Re: MHbound]
#9076642 - 10/14/08 03:28 PM (15 years, 5 months ago) |
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Quote:
MHbound said: Stick them above the fridge...It's a nice warm spot without using an incubator if you live in cold climate.
Also, it sounds like your syringe may be bad...No growth at all??? In 0 jars...I would get some new syringes.
Pressure cook your jars for 90 minutes, and if you still don't get any growth then your syringes aren't right.
What might be wrong with the syringe that would cause no growth? I am not arguing, I am just wondering. Syringes are easy to get in my area, I could go get some. The wost thing about them is the needles are too short and to get needles long enough I have to buy a big bulk of them for 20 bucks, I know that dont sound like much, but at this time it is for me. The cost of the syringes is cheap , but the needles are high.
BTW: I flame sterilize my needles witha lighter and cool it on an alcohol cloth. To re-use my syringes I pull alcohol into them , shake , and push it out into the needle cap, that way the cap gets clean too. Then i boil distilled water and triple rinse the syringe with the boiling water. The I do it a fourth time and let it cool to room temp. I keep the spore syringe for 1 or 2 days to let them hydrate before using them. Mabe the alcohol rinse is unnessisary?
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: jars wont colonize anymore [Re: lipa]
#9076649 - 10/14/08 03:30 PM (15 years, 5 months ago) |
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Just relised that you said you mixed your dry ingredients first. Its better to get the vermiculite to feild capicity first (if you get it too wet just add more dry verm) then evenly mix in the flour.
This helps evenly coat the vermiculite with rice. If you add the water last, the brf tends to clump together at the bottom of the bowl and is hard to get easily mixed vith the verm.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Re: jars wont colonize anymore [Re: lipa]
#9076666 - 10/14/08 03:35 PM (15 years, 5 months ago) |
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Quote:
lipa said: God I hate lysol!!
Try this next time.
Sterilize your substrates and add a shot glass wrapped in foil, a jar of water, your syringe opened all the way with foil around it, and whatever you are going to scrape the spores with in a piece of foil.
While the pressure cooker is cooking wipe the inside of your glove box down with alcohol and make sure the holes are covered. Keep the room clear of anything that is moving including you and let the air settle.
When you are done and the pressure cooker is cool enough to open and the pressure has dropped go inside the room and gently open the cooker and place the contents into you box. allow the substrate to cool.
Set everything but the syringe the spores and your shot glass aside and cock the spore print open but not open to expose it. Just enough to make it easy to open with one hand. loosen up the foil on top of the glass and grab your scalpel.
Take the spore print and open the glass up slowly and scrape some spores into it. Close the glass back with the foil and grab your jar of water. plunge your syringe into the water slowly and draw up water till it is full.
Take the syringe and gently squirt the water into the glass of spores and redraw. Expel the fluid and redraw a few times to help break up the spores.
Now inoculate the jars as usual.
P.s> You can use tap water. The chlorine is gone after it is done cooking. If your worried boil it first. It won't affect your spores. I promise.
I know you probably know all this already. I just want you to know you are going way overboard with the lysol and there is no need to bomb anything with it. Just keep your movements steady and smooth.
OK, I will add those extra items to the pot when I sterilze my jars of substrate. Thank you
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Re: jars wont colonize anymore [Re: veda_sticks]
#9076675 - 10/14/08 03:37 PM (15 years, 5 months ago) |
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Quote:
veda_sticks said: Just relised that you said you mixed your dry ingredients first. Its better to get the vermiculite to feild capicity first (if you get it too wet just add more dry verm) then evenly mix in the flour.
This helps evenly coat the vermiculite with rice. If you add the water last, the brf tends to clump together at the bottom of the bowl and is hard to get easily mixed vith the verm.
Ok, I will wet the verm first next time and add flour last. thanks
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seven
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Registered: 06/09/08
Posts: 1,478
Loc: north carolina
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Re: jars wont colonize anymore [Re: sumo]
#9076677 - 10/14/08 03:38 PM (15 years, 5 months ago) |
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what ever makes you feel safe man. haha alot of people over do the percautions to feel safe. tip- try looking for syringes at tractor supply co. or other stores which carry farming supplys. farmers use the syringes to shoot up cows and other livestock. the needles are longer- well you a choice of sizes.
-------------------- grind
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MHbound
Ballin Out At All Cost
Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 11 months
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Re: jars wont colonize anymore [Re: seven]
#9076711 - 10/14/08 03:45 PM (15 years, 5 months ago) |
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I don't mean the syringe itself...I'm talking about you may have a contaminated syringe.
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Re: jars wont colonize anymore [Re: seven]
#9076981 - 10/14/08 04:45 PM (15 years, 5 months ago) |
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Quote:
seven said: what ever makes you feel safe man. haha alot of people over do the percautions to feel safe. tip- try looking for syringes at tractor supply co. or other stores which carry farming supplys. farmers use the syringes to shoot up cows and other livestock. the needles are longer- well you a choice of sizes.
yeah , thats wer I get em
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alkylbenzene23
Indeed
Registered: 08/16/08
Posts: 615
Last seen: 14 years, 3 months
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Re: jars wont colonize anymore [Re: lipa]
#9077298 - 10/14/08 05:45 PM (15 years, 5 months ago) |
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Are you letting your jars cool before inoculating them?
-------------------- If you try to lick your cat while tripping, and they bite you, are you even?
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Quote:
alkylbenzene23 said: Are you letting your jars cool before inoculating them?
Absolutely , I usually steam at night and take em out after pot is cool to touch and then inoculate the next day. Thanks for your reply.
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sumo
Stranger
Registered: 10/14/08
Posts: 50
Last seen: 13 years, 10 months
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Re: jars wont colonize anymore [Re: sumo]
#9077445 - 10/14/08 06:06 PM (15 years, 5 months ago) |
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Rikktor
Stranger-Danger
Registered: 04/24/11
Posts: 9
Last seen: 12 years, 9 months
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Re: jars wont colonize anymore [Re: MHbound]
#14396176 - 05/03/11 07:13 PM (12 years, 10 months ago) |
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I had this same problem, slow recovery?
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