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Offlinegaviao
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Registered: 08/10/08
Posts: 15
Last seen: 13 years, 2 months
Bad syringe?
    #9075429 - 10/14/08 10:38 AM (16 years, 3 months ago)

Hello Shroomery.  Long time reader, first time poster.  I have an issue with some contams that I wanted to ask about.  Theyre rye jars that were sterilized in AA930 at 15psi for 80 minutes.  I used a glove box and sterile procedure. I flame sterilized the needle before every jar.  After a few days contams started to show up (trich).  The thing is that the contam is only showing up where the solution from the syringe was injected, its not showing up in any other part of the jar.  I was wondering if this was consistent with a bad syringe or not.  Thanks for any info.

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Offlinegrod31
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Registered: 12/06/07
Posts: 2,077
Loc: New York
Last seen: 15 years, 11 months
Re: Bad syringe? [Re: gaviao]
    #9075441 - 10/14/08 10:40 AM (16 years, 3 months ago)

i mean it sounds like you know what you are doing.Where did you get the syringe a vendor? was it home made?


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Invisibledjmako7
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Registered: 06/13/06
Posts: 2,362
Loc: A shady spot under a tree
Re: Bad syringe? [Re: grod31]
    #9075452 - 10/14/08 10:44 AM (16 years, 3 months ago)

It is possible to get contamed syringes although unlikely (if from a shroomery sponsor vendor)


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Offlinegaviao
Stranger
Registered: 08/10/08
Posts: 15
Last seen: 13 years, 2 months
Re: Bad syringe? [Re: djmako7]
    #9075466 - 10/14/08 10:47 AM (16 years, 3 months ago)

Yeah, the syringe was from a vendor.  I've never had a problem with contams before.  I wanted to peg exactly how these jars got contaminated.

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OfflineUnknownStrain
Stranger

Registered: 10/14/08
Posts: 10
Last seen: 14 years, 6 months
Re: Bad syringe? [Re: grod31]
    #9075474 - 10/14/08 10:51 AM (16 years, 3 months ago)

I'm assuming it is coming from inside the syringe.

What leads me to believe this, is the fact that you've sterilized
the needle tip before each inoculation. Also, using a glovebox, etc.
Only showing up at the point(s) of inoculation is another clue.

Try cleaner syringe preparations, or a different source?

A dirty print could be the culprit!

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Invisibledjmako7
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Re: Bad syringe? [Re: UnknownStrain]
    #9075489 - 10/14/08 10:58 AM (16 years, 3 months ago)

Quote:

UnknownStrain said:


A dirty print could be the culprit!




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