2:No idea never made it, perhaps try PDA?

3:hope so, I use it. I am constructing a glove box thou

4:I would think that if you can see spores landing on your agar, there are millions of spores in what you saw. Fast colonization would result from mass innoculation of the agar plate.

my 2 cents

Why did you have a vacuum in your jars? I am under the impression that the seals should go up so a little air can sneak thru. I personally would eliminate the causes of the vacuum if possible.

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Say what you will. But dammit, grandma is one hell of a good french kisser.

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-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)