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What is the disadvantage of letting the agar completely colonize? ( with the exception of not being able to to isolate a pure strain. ) I've read not to let it happen, but no reason as to why. If someone let it fully colonize and didn't refridgerate it until 5 or 6 days after complete colonization, would there be any detrimental effect on the mycellia due to lack of nutrients?
The main reason people use dishes that are not completely colonised is because the outer edge of the agar functions as an indicator for contamination. If the outer edge is clean, so will (most likely) be the mycelial mat.
Since i always tape my dishes i don't find it necessary. I check the dishes a few days after the transfer. I've not yet seen contaminants pop up after a week or so.
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