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hi everyone I haven't grown mushrooms before, though I am a huge fan. have some questions though... so hydrogen peroxide degrades spontaneously into water and o2 over time. if one were to make some pf jars with 70% or so h2o2 instead of water I was thinking that s/he would have but to wait a while for the peroxide to degrade into plain old water, and voila! sterile jar with no nasty spore killing chems.
question 1: has anyone tried this? if so, how long did you wait for the h202 to change? #2: if someone were really impatient and wanted to put some catalase in the jars to speed up the degredation, would contams be a problem if he just shot some blood or saliva mixed in a squeezle more h2o2 into the jars?
ok, so it looks like there's a book out there that uses h2o2 to keep sterile, but I dont really want to buy it since the first question of the above post is all my friend of a friend really would like to know before he starts experimenting. he has a limited amount of spores and would like to keep from killing them if possible.
hydrogen peroxide will a: break down too rapidly without sterilizing the insides of whatever particles are in your jar, because it reacts violently with organic material of any sort b: probably render the organic material the mycellium would feed on inedible because of its broken down state, and would possibly create substances prohibitive to mycellial growth.
LOL. 70% h2o2 is hard to come by. What you buy in the store is 3%. 35% h2o2 is used in the food industry. Most larger chemical suppliers sell 50% for lab use . 95% h2o2 /5% hydrazine (a chemical stabalizer, this concentration of h2o2 is somewhat unstable) is used a a fuel oxidizer, ie. rocket propellent. I suggest you do some more research and stick to established methods.
-------------------- If you grow them, they will come.
thanks everyone. the friend of a friend might give it a whirl anyway when he gets some prints, but thanks for your advice. if it does in fact work, I'll be sure to say 'I told you so.' if anyone actually has tried to do this, please let me know. btw, h2o2 is carried in a lot of small chem supply shops and really isn't hard to get in high concentrations.