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Offlinegermplasm
Stranger
Registered: 09/24/02
Posts: 3
Last seen: 15 years, 1 month
h2o2
    #904956 - 09/24/02 04:51 PM (15 years, 2 months ago)

hi everyone
I haven't grown mushrooms before, though I am a huge fan. have some questions though...
so hydrogen peroxide degrades spontaneously into water and o2 over time. if one were to make some pf jars with 70% or so h2o2 instead of water I was thinking that s/he would have but to wait a while for the peroxide to degrade into plain old water, and voila! sterile jar with no nasty spore killing chems.

question 1: has anyone tried this? if so, how long did you wait for the h202 to change?
#2: if someone were really impatient and wanted to put some catalase in the jars to speed up the degredation, would contams be a problem if he just shot some blood or saliva mixed in a squeezle more h2o2 into the jars?

thanks in advance


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Offlinegermplasm
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Registered: 09/24/02
Posts: 3
Last seen: 15 years, 1 month
Re: h2o2 [Re: germplasm]
    #905034 - 09/24/02 05:16 PM (15 years, 2 months ago)

ok, so it looks like there's a book out there that uses h2o2 to keep sterile, but I dont really want to buy it since the first question of the above post is all my friend of a friend really would like to know before he starts experimenting. he has a limited amount of spores and would like to keep from killing them if possible.


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OfflineMajor_Buzz
just like that

Registered: 02/14/02
Posts: 190
Loc: Left Bank
Last seen: 12 years, 4 months
Re: h2o2 [Re: germplasm]
    #905037 - 09/24/02 05:20 PM (15 years, 2 months ago)

I think you should stick to a tried and true method, and sterilize with a pressure cooker.


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Invisibleblazenaz
Extremist

Registered: 07/29/02
Posts: 309
Loc: Surrounded by an electrom...
Re: h2o2 [Re: Major_Buzz]
    #905186 - 09/24/02 06:31 PM (15 years, 2 months ago)

2nd that...


--------------------
Blaze


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Offlinevalkyrie
journeyman

Registered: 10/29/01
Posts: 98
Loc: Midwesternly
Last seen: 4 years, 1 month
Re: h2o2 [Re: blazenaz]
    #905197 - 09/24/02 06:38 PM (15 years, 2 months ago)

hydrogen peroxide will
a: break down too rapidly without sterilizing the insides of whatever particles are in your jar, because it reacts violently with organic material of any sort
b: probably render the organic material the mycellium would feed on inedible because of its broken down state, and would possibly create substances prohibitive to mycellial growth.



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InvisibleAction_Man
RIP Houser

Registered: 01/24/02
Posts: 91
Re: h2o2 [Re: valkyrie]
    #905259 - 09/24/02 07:14 PM (15 years, 2 months ago)

No shortcuts in this hobby. Your wasting your time & effort if you experiment while starting off. Follow the Tek & boil/PC the jars.


--------------------
Cookin up some fuel for us all. :laugh:


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Invisibleoverclock
beekeeper
Registered: 09/04/02
Posts: 17
Re: h2o2 [Re: germplasm]
    #905558 - 09/24/02 10:31 PM (15 years, 2 months ago)

LOL. 70% h2o2 is hard to come by. What you buy in the store is 3%. 35% h2o2 is used in the food industry. Most larger chemical suppliers sell 50% for lab use . 95% h2o2 /5% hydrazine (a chemical stabalizer, this concentration of h2o2 is somewhat unstable) is used a a fuel oxidizer, ie. rocket propellent. I suggest you do some more research and stick to established methods.

Peace.
-oc


--------------------
If you grow them, they will come.


Edited by overclock (09/24/02 10:32 PM)


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Offlinegermplasm
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Registered: 09/24/02
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Re: h2o2 [Re: overclock]
    #905677 - 09/24/02 11:29 PM (15 years, 2 months ago)

thanks everyone.
the friend of a friend might give it a whirl anyway when he gets some prints, but thanks for your advice.  if it does in fact work, I'll be sure to say 'I told you so.'  :smile:  if anyone actually has tried to do this, please let me know.  btw, h2o2 is carried in a lot of small chem supply shops and really isn't hard to get in high concentrations.


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OfflineMajor_Buzz
just like that

Registered: 02/14/02
Posts: 190
Loc: Left Bank
Last seen: 12 years, 4 months
Re: h2o2 [Re: germplasm]
    #907038 - 09/25/02 12:43 PM (15 years, 2 months ago)

Finally dude you have to cook the grains. How you goona cook em without using any water?


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