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Offlinealkylbenzene23
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Spore inoculated LC: stir or no stir
    #9048658 - 10/08/08 04:18 PM (16 years, 3 months ago)

I'm wondering whether I should place my spore inoculated LC's on my home brew magnetic stirrer, or if I should just leave them along and let them colonize. Also, how long should it be until you see mycelium with LCs?


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OfflineJazzCatCF
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Re: Spore inoculated LC: stir or no stir [Re: alkylbenzene23]
    #9049120 - 10/08/08 05:26 PM (16 years, 3 months ago)

you should start seeing myc in 5 to 10 days if in proper temp.it might very depending on your lc ingredients.once mine start getting slightly thick I spin,just to break it up,then I dont spin again till ready to suck into syringe.works fine for me.


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Invisiblesandman420
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Re: Spore inoculated LC: stir or no stir [Re: JazzCatCF]
    #9049184 - 10/08/08 05:35 PM (16 years, 3 months ago)

For the fastest colonization times (3-4 days) stir 24/7 at about 50-300 rpm or so as long as your stirrer does not heat up the culture obviously. THis is where a ceramic top stirrer comes in handy.


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Offlinealkylbenzene23
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Re: Spore inoculated LC: stir or no stir [Re: sandman420]
    #9049360 - 10/08/08 06:06 PM (16 years, 3 months ago)

What is the proper temp range for LCs? Right now they are in my closet which made a perfect colonizing chamber for BRF.


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Offlineveda_sticks
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Re: Spore inoculated LC: stir or no stir [Re: alkylbenzene23]
    #9049372 - 10/08/08 06:08 PM (16 years, 3 months ago)

Same conditions as your jars.


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InvisibleDoseMeHomie
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Re: Spore inoculated LC: stir or no stir [Re: veda_sticks]
    #9091863 - 10/17/08 02:14 PM (16 years, 3 months ago)



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