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Invisibleteknix
π“‚€βŸπ“…’π“π“…ƒπ“Š°π“‰‘ 𓁼𓆗⨻
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Registered: 09/16/08
Posts: 11,953
My homeade self healing injection port.
    #9039478 - 10/06/08 09:37 PM (15 years, 5 months ago)

What I do is punch a hole in the lid where I want my injection port.

I take a piece of polyfil and twist it as tightly as I can , then put it into the hole.

I trim both side of the excess polyfil off with siscors.

Then I briefly use my lighter to (compact the polyfil and give it a more uniform surface.

I apply a liberal amount of silicone to either side of the polyfil, making sure the entire surface is covered.

Before injection I will suck a small amount of alchohol (70%) with a syringe and inject it into the polyfil, then continue through the bottom layer of the silicone and inject it.

I know u can just use the silicone alone and it would work, but I feel this will give me an added barrier against contamination while inserting and removing the needle.

I want to eventually try this without applying flame sterilazion, without a still air box. However at this point, Im still new to it all and will use a still air bx and flame sterilization.

Any thoughts of comments?

I appologize if this has already been posted, I just didnt see it.

Β© Tuesday, October 06, 2008


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

Edited by teknix (10/07/08 12:03 AM)

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Offlinefungiamongi2
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Re: My homeade self healing injection port. [Re: teknix]
    #9039526 - 10/06/08 09:47 PM (15 years, 5 months ago)

so you have polyfil covered in silicone?    seems like it would be prone to more contams b/c there is extra stuff to hold anything that contams, and when that gets wet, how is it gonna dry?    i could be way off too.


--------------------
gonna bring her a kiss, make those blues run

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InvisibleTheShroomJew23
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Re: My homemade self healing injection port. [Re: fungiamongi2]
    #9039539 - 10/06/08 09:50 PM (15 years, 5 months ago)

When you PC polyfill it melts  into a impenetrable puck.


--------------------


You will be missed

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OfflineJuke Adro
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Re: My homemade self healing injection port. [Re: TheShroomJew23]
    #9039562 - 10/06/08 09:55 PM (15 years, 5 months ago)

Quote:

TheShroomJew23 said:
When you PC polyfill it melts  into a impenetrable puck.


Rubbish it does not melt at all it can with stand 20psi


--------------------
Someone said:  im actually not using ms, im using prints.
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OfflineJeets
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Re: My homemade self healing injection port. [Re: Juke Adro]
    #9039573 - 10/06/08 09:57 PM (15 years, 5 months ago)

Quote:

Juke Adro said:
Quote:

TheShroomJew23 said:
When you PC polyfill it melts  into a impenetrable puck.


Rubbish it does not melt at all it can with stand 20psi





Yes agreed it can withstand heat well. The only thing is you may get a little condensation in it, that's all

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Invisibleteknix
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Re: My homemade self healing injection port. [Re: Jeets]
    #9039595 - 10/06/08 10:01 PM (15 years, 5 months ago)

The thing that worry's me the most is if I dont get all the alchohol out in the polyfil and squirt some into into my LC. I dont know if a minute amount could spell catastrophe?!? If so, I will take extra precaution and inject a little more  than what I sucked up into the polyfil layer.

Edited by teknix (10/07/08 03:29 PM)

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Offlineneybis
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Re: My homemade self healing injection port. [Re: Jeets]
    #9039599 - 10/06/08 10:02 PM (15 years, 5 months ago)

im really curious if this would work or not because i was planning on starting LC's soon and need the self healing ports. Any old hands have any input as to whether or not this could possibly lead to contams due to the fact that it might hold IN the moisture??


--------------------
"Why is there so much controversy about drug testing? I know plenty of guys who would be willing to test any drug they could come up with."

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Invisibleteknix
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Re: My homeade self healing injection port. [Re: teknix]
    #9039633 - 10/06/08 10:11 PM (15 years, 5 months ago)

Also instead of adding a filter for gas exchange, I plan on injecting sterile air directly into the jar once every so often.

To do this I will suck a small amount of alcohol into the syringe. I will then light a candle, stick my needle into the flame to sterilize it. I will raise the needle a few inches above the flame and suck in air. I will then inject the alcohol into the polyfil layer, then proceed throught the bottom layer of silicone and inject the air.

I dont know if a positive pressure environment would be good or bad. I plan to test this as soon as my spores/myc arrive.

Edited by teknix (10/07/08 03:27 PM)

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InvisibleTheShroomJew23
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Re: My homeade self healing injection port. [Re: teknix]
    #9039646 - 10/06/08 10:16 PM (15 years, 5 months ago)

My polyfill always melts, thats why I only use tyvek and micropore tape. I love tyvek so much anyways.


--------------------


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Invisibleteknix
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Re: My homeade self healing injection port. [Re: TheShroomJew23]
    #9039668 - 10/06/08 10:20 PM (15 years, 5 months ago)

Quote:

TheShroomJew23 said:
My polyfill always melts, thats why I only use tyvek and micropore tape. I love tyvek so much anyways.




If this is the case, I will try it with a fresh filter from a cigarette. I hope they both dont melt, otherwise its back to the drawing board :P.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

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OfflineDamion5050
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Re: My homeade self healing injection port. [Re: teknix]
    #9039694 - 10/06/08 10:25 PM (15 years, 5 months ago)

if they keep melting invest is some synthetic filter discs from mycosupply

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InvisibleTheShroomJew23
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Re: My homeade self healing injection port. [Re: Damion5050]
    #9039709 - 10/06/08 10:28 PM (15 years, 5 months ago)

Don't use cig filters, they rot. They are meant to biodegrade now and they will just turn to mush in not long.


--------------------


You will be missed

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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: TheShroomJew23]
    #9039737 - 10/06/08 10:31 PM (15 years, 5 months ago)

Ear plugs work


--------------------
Someone said:  im actually not using ms, im using prints.
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Invisibleteknix
π“‚€βŸπ“…’π“π“…ƒπ“Š°π“‰‘ 𓁼𓆗⨻
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Re: My homeade self healing injection port. [Re: Juke Adro]
    #9039779 - 10/06/08 10:40 PM (15 years, 5 months ago)

Quote:

Juke Adro said:
Ear plugs work




Good call there. I actually have some of these laying around here somewhere.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

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Offlinehowsitgoingtoend
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Re: My homemade self healing injection port. [Re: teknix]
    #9039898 - 10/06/08 11:07 PM (15 years, 5 months ago)

I've lived in some shit holes and jar contamination was never a huge problem. On Average I'd get 2-3 jars out of 8 that went south but that wasn't bad considering the environment and my lack of any fancy equipment. Save your money and stick with the vermiculite layer and four small holes with tape over them.

I'd worry more about the sterile syringe making process.

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Offlinehaze666
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Re: My homemade self healing injection port. [Re: howsitgoingtoend]
    #9039916 - 10/06/08 11:10 PM (15 years, 5 months ago)

What is the point of pf in the middle of the silicone?  I heard of a squirrel having a 100% success rate with silicone and no poly.

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Invisibletripchip
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Re: My homemade self healing injection port. [Re: haze666]
    #9039955 - 10/06/08 11:20 PM (15 years, 5 months ago)

you got the wrong polyfill if it melts.


--------------------
Psilocybe Cubensis

Psilocybe comes from the Greek root "psilos" meaning bald head and Cubensis because it was first recognized as a new species from specimens collected in Cuba.

                                    :mushroom2:CHIP:mushroom2:

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Invisibleteknix
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Re: My homemade self healing injection port. [Re: haze666]
    #9039987 - 10/06/08 11:28 PM (15 years, 5 months ago)

Quote:

haze666 said:
What is the point of pf in the middle of the silicone?  I heard of a squirrel having a 100% success rate with silicone and no poly.



Actually the intention is to minimize the sterile procedures when injecting LC, although it may be able to be applied to other applications as well.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

Edited by teknix (10/06/08 11:36 PM)

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Offlineveda_sticks
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Re: My homemade self healing injection port. [Re: teknix]
    #9040635 - 10/07/08 03:58 AM (15 years, 5 months ago)

You will still have to flame sterilise before inoculating each jar. Just because you have alcohol in the polyfill between your silicon is not going to sterilise you needle.

We flame the needle red hot not to kill anything that has landed on the needle, but anything that may have worked its way inside the needle.

Always flame your needle red hot. You can make your own alcohol lamp with a 1/2 pint canning jar. Punch a small hole in the lid and feed a peice of string through the lid so the string sits in the alcohol and you have a little bit through the top. Light.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisibleteknix
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Re: My homemade self healing injection port. [Re: veda_sticks]
    #9040669 - 10/07/08 04:31 AM (15 years, 5 months ago)

Good morning veda, see you've been busy ^^

I realize that alcohol sanitizes. This is more for an added layer of protection, as LC is highly contaminable.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

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Offlineveda_sticks
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Re: My homemade self healing injection port. [Re: teknix]
    #9040691 - 10/07/08 04:41 AM (15 years, 5 months ago)

LC's are no more contaminant prone than jars of substrate. An LC is just as easy or likely to contaminate than substrates or agar etc.

They are just much harder to spot contamination with. Moulds wont sporolate in liquid culture.

If you want a self healing injection port, just stick with using rtv silicone. Adding polyfill will add no extra protection and id be worried about the polyfill reducing its effectivnesss in resealing.
Silicone alone has proven to be very effective and see no need to add anything else. Certainly wont remove the need to flame sterise you needle.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleTheShroomJew23
The Chemist
Male

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Re: My homemade self healing injection port. [Re: veda_sticks]
    #9042157 - 10/07/08 12:36 PM (15 years, 5 months ago)

I have never had a LC contam on me and I dont even PC them most of the time.


--------------------


You will be missed

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Offlinenenl
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Re: My homemade self healing injection port. [Re: TheShroomJew23]
    #9042216 - 10/07/08 12:46 PM (15 years, 5 months ago)

I use silicone on both sides of the lid with nothing in between and it's worked great. Punch 2 holes with a paper hole punch , silicone one hole and tyvec the top AND bottom of the lid. So far no problems with a good dozen or so LCs and probably 3x that grain jars.

I'm not a big fan of manually adding fresh air even if it is sterile because you never really know... I have been known to inject LC solution in to replace what I take out though.


--------------------
hehe

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Offlinefungiamongi2
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Re: My homemade self healing injection port. [Re: nenl]
    #9042251 - 10/07/08 12:53 PM (15 years, 5 months ago)

would you like to breathe alcohol vapor air?


--------------------
gonna bring her a kiss, make those blues run

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Invisibleohmatic
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Re: My homemade self healing injection port. [Re: tripchip]
    #9042380 - 10/07/08 01:19 PM (15 years, 5 months ago)

Quote:

tripchip said:
you got the wrong polyfill if it melts.




i second that. polyfill is made of Poly Propylene, the same which spawnbags are made of
and spawnbags dont melt either so why should polyfill.


--------------------
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Invisibleteknix
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Re: My homemade self healing injection port. [Re: fungiamongi2]
    #9042479 - 10/07/08 01:39 PM (15 years, 5 months ago)

Quote:

fungiamongi2 said:
would you like to breathe alcohol vapor air?




I dont think the alcohol vapor will go into the jar, as the positive pressure should push the vapor out of the jar, at the same time dissipating most of what was squirted into the polyfil layer.

Edited by teknix (10/07/08 03:25 PM)

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Re: My homeade self healing injection port. [Re: teknix]
    #9043347 - 10/07/08 04:30 PM (15 years, 5 months ago)

Can anyone think of a test I could run on these jars without myc/spores? To see if the will contam? I have some hominy, vermiculite, honey, and patato flakes atm.


--------------------
.6th and 7th sense theory
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Re: My homeade self healing injection port. [Re: teknix]
    #9043507 - 10/07/08 04:57 PM (15 years, 5 months ago)

Leave it for a week if something grows it's no good, I read a article on how to mass produce mycelium and that's pretty much what they said, I think this may be it dunno cant be bothered reading tho


STARTING THE CULTURE
Upon obtaining one or more specimens of a psilocybin bearing mushroom one can begin to cultivate as much of the hallucinogen as is desired. Any part of the fungus can be used for inoculation. If the spores are used, consideration must be given to the natural life cycle of the mushroom. A single cap contains millions of spores, and any one of these will germinate in the medium to form a mycelium. But this mycelium will consist of what is known as monokaryotic tissue. Such a mycelial organism will grow for a while, but unless it mates with another compatible monokaryotic mycelium and forms a dikaryotic structure it will eventually perish. To develop a culture from spores proceed as follows: Scrape the spores from the print into about 10 ml of sterilized water. Shake well. Add 90 ml of sterilized water and shake again. There will be millions of spores in this solution. Have ready several petri dishes or other suitable containers as described previously containing sterilized agar medium. Lift the lid slightly on each container and with a sterilized pipette or syringe place a drop of this spore water on three or four different parts of the agar surface, then cover the container immediately. Let the dish stand at room temperature for 3-5 days. Radial growths of monokaryotic mycelium will occur at each inoculation point. When any two mycelia have grown to the point of making contact with each other mating (somatogamy) has taken place and within a few days these united mycelia will have become dikaryotic organisms. Any portion of this mycelial tissue can now be used to seed new cultures as described later.

If a portion of one of the carpaphores gathered in the field is used to inoculate the agar, mating is not required. The tissue of mature fungus is, of course, already dikaryotic. To avoid contamination only inner tissue is used. Place the mushroom cap gills-down on a clean work area at least three feet away from any equipment. Wipe all dirt and slime from the cap with a Q-tip and swab it's whole surface with a seven percent iodine solution. Pin the cap to the Table top by inserting three disecting needles at equilateral points. Sterilize an X-acto blade in a flame, let it cool for a moment, then carve the outer skin of the mushroom. Cut out tiny pieces of the inner mushroom flesh each the size of a match head. Spear each piece with the blade point, raise the lid of the petri dish slightly, press the tissue firmly into the agar surface, and close the lid immediately. Place all dishes so inoculated on the incubation shelf at room temperature. The mycelium must breath as it grows, so do not cap the lids too tightly. When the radial growth of the mycelia appear on the agar surface (3-5 days) these stock cultures are ready for transferring to the broth jars. If any stock cultures are not going to be used immediately, tighten their lids and place them under refrigeration. They can be kept this way for about a year.
RAISING CROP CULTURES OF MYCELIA
The task now is to select the most vigorous appearing mycelia in the dishes. This means the largest and fastest growing specimens and, of course, those not contaminated by foreign molds. Contaminants are not difficult to detect because their appearance differs greatly from that of the mycelia. Mycelia are pure white fibrous mats sometimes having a light bluish tinge.

Contaminants may appear as rapid-growing, tiny, white circular spots with blue-green centers, or as surface scums or fuzzy clusters of either gray, black, yellow, green, or blue color. If any contamients appear in any of the culture dishes, discard those cultures. When the dishes containing the choicest mycelia have been selected the mycelia can be transferred from the agar-based stock cultures to the liquid broth cultivation jars. These jars should have been prepared and sterilized three days before transferring and allowed to stand at room temperature during this time to test the effectivness of sterilization by observing if contaminates appear. Discard all broths which contain growths. The uncontaminated jars are now ready for inoculation. Spray the room and clean the work area as described under pure culture techniques. Also spray the outside parts of the stock dishes and culture jars. Lift the lid of a stock dish just enough and pick up a fragment of mycelium with an inoculation loop that has been flame sterilized and allowed a moment to cool. Lift the cover of the jar, place the mycelium fragment in the broth and cover the jar immediately. Repeat this until all jars have been inoculated. Refrigerate all unused stock cultures. Tighten the jar covers and shake well to disperse the inoculum throughout the broth. This also aerates the medium; the mycelium needs oxygen for life support and growth. Loosen the lids again and place the jars on the growing shelf for 10-12 days at 70-75 degrees F. If other species than Psilocybe cubensis are used, adjust the temperature accordingly. Every 2-3 days tighten the jar covers, shake to aerate and disperse mycelium, reloosen the covers, and return the jars to the shelf.

The process of growth can be followed with a saccharimeter. The maximum growth and highest percentage of psilocybin occurs about four days after all of the broth's sugar content has been used up. The mycelium should be harvested at this time. Any jars that cannot be harvested on that day should be refrigerated until this can be done.


--------------------
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Invisibleteknix
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Re: My homeade self healing injection port. [Re: Juke Adro]
    #9043683 - 10/07/08 05:25 PM (15 years, 5 months ago)

Quote:

Juke Adro said:
Leave it for a week if something grows it's no good, I read a article on how to mass produce mycelium and that's pretty much what they said, I think this may be it dunno cant be bothered reading tho


STARTING THE CULTURE
Upon obtaining one or more specimens of a psilocybin bearing mushroom one can begin to cultivate as much of the hallucinogen as is desired. Any part of the fungus can be used for inoculation. If the spores are used, consideration must be given to the natural life cycle of the mushroom. A single cap contains millions of spores, and any one of these will germinate in the medium to form a mycelium. But this mycelium will consist of what is known as monokaryotic tissue. Such a mycelial organism will grow for a while, but unless it mates with another compatible monokaryotic mycelium and forms a dikaryotic structure it will eventually perish. To develop a culture from spores proceed as follows: Scrape the spores from the print into about 10 ml of sterilized water. Shake well. Add 90 ml of sterilized water and shake again. There will be millions of spores in this solution. Have ready several petri dishes or other suitable containers as described previously containing sterilized agar medium. Lift the lid slightly on each container and with a sterilized pipette or syringe place a drop of this spore water on three or four different parts of the agar surface, then cover the container immediately. Let the dish stand at room temperature for 3-5 days. Radial growths of monokaryotic mycelium will occur at each inoculation point. When any two mycelia have grown to the point of making contact with each other mating (somatogamy) has taken place and within a few days these united mycelia will have become dikaryotic organisms. Any portion of this mycelial tissue can now be used to seed new cultures as described later.

If a portion of one of the carpaphores gathered in the field is used to inoculate the agar, mating is not required. The tissue of mature fungus is, of course, already dikaryotic. To avoid contamination only inner tissue is used. Place the mushroom cap gills-down on a clean work area at least three feet away from any equipment. Wipe all dirt and slime from the cap with a Q-tip and swab it's whole surface with a seven percent iodine solution. Pin the cap to the Table top by inserting three disecting needles at equilateral points. Sterilize an X-acto blade in a flame, let it cool for a moment, then carve the outer skin of the mushroom. Cut out tiny pieces of the inner mushroom flesh each the size of a match head. Spear each piece with the blade point, raise the lid of the petri dish slightly, press the tissue firmly into the agar surface, and close the lid immediately. Place all dishes so inoculated on the incubation shelf at room temperature. The mycelium must breath as it grows, so do not cap the lids too tightly. When the radial growth of the mycelia appear on the agar surface (3-5 days) these stock cultures are ready for transferring to the broth jars. If any stock cultures are not going to be used immediately, tighten their lids and place them under refrigeration. They can be kept this way for about a year.
RAISING CROP CULTURES OF MYCELIA
The task now is to select the most vigorous appearing mycelia in the dishes. This means the largest and fastest growing specimens and, of course, those not contaminated by foreign molds. Contaminants are not difficult to detect because their appearance differs greatly from that of the mycelia. Mycelia are pure white fibrous mats sometimes having a light bluish tinge.

Contaminants may appear as rapid-growing, tiny, white circular spots with blue-green centers, or as surface scums or fuzzy clusters of either gray, black, yellow, green, or blue color. If any contamients appear in any of the culture dishes, discard those cultures. When the dishes containing the choicest mycelia have been selected the mycelia can be transferred from the agar-based stock cultures to the liquid broth cultivation jars. These jars should have been prepared and sterilized three days before transferring and allowed to stand at room temperature during this time to test the effectivness of sterilization by observing if contaminates appear. Discard all broths which contain growths. The uncontaminated jars are now ready for inoculation. Spray the room and clean the work area as described under pure culture techniques. Also spray the outside parts of the stock dishes and culture jars. Lift the lid of a stock dish just enough and pick up a fragment of mycelium with an inoculation loop that has been flame sterilized and allowed a moment to cool. Lift the cover of the jar, place the mycelium fragment in the broth and cover the jar immediately. Repeat this until all jars have been inoculated. Refrigerate all unused stock cultures. Tighten the jar covers and shake well to disperse the inoculum throughout the broth. This also aerates the medium; the mycelium needs oxygen for life support and growth. Loosen the lids again and place the jars on the growing shelf for 10-12 days at 70-75 degrees F. If other species than Psilocybe cubensis are used, adjust the temperature accordingly. Every 2-3 days tighten the jar covers, shake to aerate and disperse mycelium, reloosen the covers, and return the jars to the shelf.

The process of growth can be followed with a saccharimeter. The maximum growth and highest percentage of psilocybin occurs about four days after all of the broth's sugar content has been used up. The mycelium should be harvested at this time. Any jars that cannot be harvested on that day should be refrigerated until this can be done.




lol, thats cloning dude :P. I think im going to just mix some 3% honey LC and set it in the incubator at about 90F, That should make it show itself.

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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: teknix]
    #9044047 - 10/07/08 06:35 PM (15 years, 5 months ago)

LOL like I said I didn't read it but it does say mycelia can be transferred from the agar-based stock cultures to the liquid broth cultivation jars. These jars should have been prepared and sterilized three days before transferring and allowed to stand at room temperature during this time to test the effectivness of sterilization by observing if contaminates appear. Discard all broths which contain growths. The uncontaminated jars are now ready for inoculation. Spray the room and clean the work area as described under pure culture techniques. Also spray the outside parts of the stock dishes and culture jars. Lift the lid of a stock dish just enough and pick up a fragment of mycelium with an inoculation loop that has been flame sterilized and allowed a moment to cool. Lift the cover of the jar, place the mycelium fragment in the broth and cover the jar immediately. Repeat this until all jars have been inoculated. Refrigerate all unused stock cultures. Tighten the jar covers and shake well to disperse the inoculum throughout the broth. This also aerates the medium; the mycelium needs oxygen for life support and growth. Loosen the lids again and place the jars on the growing shelf for 10-12 days at 70-75 degrees F. If other species than Psilocybe cubensis are used, adjust the temperature accordingly. Every 2-3 days tighten the jar covers, shake to aerate and disperse mycelium, reloosen the covers, and return the jars to the shelf.


--------------------
Someone said:  im actually not using ms, im using prints.
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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: Juke Adro]
    #9044102 - 10/07/08 06:42 PM (15 years, 5 months ago)



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Invisibleroquet
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Re: My homeade self healing injection port. [Re: teknix]
    #9044149 - 10/07/08 06:52 PM (15 years, 5 months ago)

Quote:

teknix said:
I dont think the alcohol vapor will go into the jar, as the positive pressure should push the vapor out of the jar [...]



How could you have positive pressure in a jar? You get a vacuum, the opposite of positive pressure, if you PC an airtight jar with the lid screwed on. As far as I know, the only time people talk about positive pressure in cultivation is pumping air into a fruiting chamber.

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InvisibleJrsxt
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Re: My homeade self healing injection port. [Re: Juke Adro]
    #9044161 - 10/07/08 06:53 PM (15 years, 5 months ago)

Are those yours?

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Offlineveda_sticks
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Re: My homeade self healing injection port. [Re: Juke Adro]
    #9044167 - 10/07/08 06:54 PM (15 years, 5 months ago)

I wouldnt buy that presterilised stuff of ebay. It may be good, but you can make it yourself for pennys. And the comment about not seeing growth from spores for 2 weeks is just plain wrong.

If your not seeing significant growth by 2 weeks somethings wrong with your substrate.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: Jrsxt]
    #9044173 - 10/07/08 06:56 PM (15 years, 5 months ago)

No, that person is in US I'm an Aussie but fcuk if someone cant get a clean product that may help


--------------------
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InvisibleJrsxt
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Re: My homeade self healing injection port. [Re: veda_sticks]
    #9044174 - 10/07/08 06:56 PM (15 years, 5 months ago)

Quote:

veda_sticks said:
I wouldnt buy that presterilised stuff of ebay. It may be good, but you can make it yourself for pennys. And the comment about not seeing growth from spores for 2 weeks is just plain wrong.

If your not seeing significant growth by 2 weeks somethings wrong with your substrate.




I agree completely. It takes that guy all of 1-2 dollars to make that. It's simple to make your own.

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InvisiblePrisoner#1
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Re: My homeade self healing injection port. [Re: teknix]
    #9044194 - 10/07/08 06:59 PM (15 years, 5 months ago)

Quote:

teknix said:
I apply a liberal amount of silicone to either side of the polyfil




instead of sillycones try polyurethane caulk, it has better
adhesion properties, is 100x tougher, and will last longer
than the lids

available at home depot


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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: veda_sticks]
    #9044209 - 10/07/08 07:02 PM (15 years, 5 months ago)

Quote:

veda_sticks said:
It may be good, but you can make it yourself for pennys.




That's my point, ppl can make it for pennies tho they cant get it clean or be bothered, so it is a substitute for noobs who can buy that and a spore syringe then have no dramas and no worries.
If I lived I US I would not even have a PC everything would be bought I'm lazy and would rather fork out 100 clams on some bags and a syringe than make them my self for a quarter the price.....see what I'm saying?


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Re: My homeade self healing injection port. [Re: Prisoner#1]
    #9044220 - 10/07/08 07:03 PM (15 years, 5 months ago)

Nice tip :-) i will be using that for LC's i will be making up for me starting to grow again.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: Prisoner#1]
    #9044232 - 10/07/08 07:06 PM (15 years, 5 months ago)

Quote:

Prisoner#1 said:
Quote:

teknix said:
I apply a liberal amount of silicone to either side of the polyfil




instead of sillycones try polyurethane caulk, it has better
adhesion properties, is 100x tougher, and will last longer
than the lids

available at home depot






Looks good.

Is that the same as sikaflex?


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Offlinenenl
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Re: My homeade self healing injection port. [Re: Juke Adro]
    #9044235 - 10/07/08 07:07 PM (15 years, 5 months ago)

If you want to get one going in a week, get it from ebay. If you want to get 12 going tonight make it yourself. $15-$20 for the premade with shipping or $10 for a dozen jars, silicone and karo. Make 12 for half the cost of one, noc them up with .5 cc each and by the time the ebay jars show up, you will probably see some growth. Some might contam from being inexperienced, but you have 12 so chances are at least one will be ok. Although it's not really that hard to make a sterile LC if you follow the info found via search in 2 minutes...

:2cents:


--------------------
hehe

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OfflineKanji
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Re: My homeade self healing injection port. [Re: nenl]
    #11665784 - 12/16/09 10:54 PM (14 years, 3 months ago)

Well, like said you can't always see contaminations in a LC. I am going to assume that most contams come during inoc and are not residual in the jar, PC tends to handle that. Well if you use fractional sterilization. So buying it on ebay might be worthwhile if your source does fractional sterilization and you don't.

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Re: My homeade self healing injection port. [Re: Kanji]
    #11665793 - 12/16/09 10:56 PM (14 years, 3 months ago)

What was the point in bumping this 1 year old post

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InvisibleOptx
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Re: My homeade self healing injection port. [Re: Shea25]
    #11666366 - 12/17/09 01:58 AM (14 years, 3 months ago)

OLD THREAD IS OLD. KANJI, PLEASE LEARN SOME FORUM ETIQUETTE.
:beatadeadhorse:

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OfflineMontock
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Re: My homeade self healing injection port. [Re: Shea25]
    #11666429 - 12/17/09 02:29 AM (14 years, 3 months ago)

Quote:

Shea25 said:
What was the point in bumping this 1 year old post



for funziez, who knows maybe he is new and isn't aware of the thread dates or something


--------------------
Looking for Wife, please pm me your picture and list of skillz.


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