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Offlinesutec
addict
Registered: 09/06/99
Posts: 301
Last seen: 13 years, 3 months
Re: Mycelium growing into a liquid media
    #90357 - 10/28/99 06:08 PM (22 years, 11 months ago)

You should check out the grow/find section of this site. There's a lot of info. Here's one tek for doing it: http://www.shroomery.org/findorgrowthem/liquidmycelium.html


--------------------
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DRCNet
MPP
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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90359 - 11/05/99 08:26 PM (22 years, 10 months ago)

Talk to this man.
shroom_wizard@yahoo.com


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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90360 - 11/09/99 09:19 PM (22 years, 10 months ago)

Yeah!
I did it!
I just used honey!!!! and it works!!!

1 t/spoon honey
1/2 pint spring water



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InvisiblePrellgott
addict
Registered: 02/09/00
Posts: 383
Re: Mycelium growing into a liquid media [Re: sutec]
    #90361 - 11/10/99 05:24 PM (22 years, 10 months ago)

...with honey..? got to try that!!


--------------------
i'm back


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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90362 - 11/13/99 01:29 PM (22 years, 10 months ago)

Just honey??? No malt extract? no agar?
Can you detail the events? You may've invented a new tek!


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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90363 - 11/13/99 04:18 PM (22 years, 10 months ago)

Simply i dissolved a teaspoon of honey (light yellow, not brown) into 250 cc of spring water...not distilled or tap....
I bought the honey at health food store...
after mix,close the jar with a lid...sterilize in pressure cooker...let it cool then inject 2-3 cc of sporeswater....
Put in a dark warm place and wait.....
In few days u will see small white hairs....this is the mycelium. Shake the jar twice a day and wait 7-10 days.....
Now the solution it's ready.....
Use it to inject pf style jars or try the whole grain tek ( i use boiled brown rice).
My pf jars are half colonized after one week from injection!!!!
Keep the jar in the fridge and u can use it for months......

Peace to all the shroomybrothers!!



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OfflineSamuel J
Mullet Poacher
Registered: 09/29/99
Posts: 19
Loc: The Commonwealth
Last seen: 20 years, 1 month
Re: Mycelium growing into a liquid media [Re: sutec]
    #90364 - 11/13/99 10:35 PM (22 years, 10 months ago)

I think this is an excellent idea. I have never seen honey get moldy, yet it contains lots of simple sugars and nutrients that the mycellium needs to grow. I'm gonna try this one soon, and see if I get the same results.

Sam



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Offlinephree_1
Stranger
Registered: 06/03/01
Posts: 64
Last seen: 21 years, 3 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90365 - 11/14/99 02:33 AM (22 years, 10 months ago)

Club99,
That is a excellent idea!!!!! Can't wait to try it.


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OfflineSneakAss
journeyman
Registered: 11/02/99
Posts: 32
Last seen: 21 years, 4 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90366 - 11/14/99 11:22 PM (22 years, 10 months ago)

This does sound like an excellent idea. Since honey is like a natural preservative, it might prevent contaminations. This could really be a breakthrough.


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Offlinephree_1
Stranger
Registered: 06/03/01
Posts: 64
Last seen: 21 years, 3 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90368 - 11/16/99 04:35 AM (22 years, 10 months ago)

Club99,
Please help!!!!!!!

[This message has been edited by phree_1 (edited November 15, 1999).]



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Offlinephree_1
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Registered: 06/03/01
Posts: 64
Last seen: 21 years, 3 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90369 - 11/16/99 04:40 AM (22 years, 10 months ago)

Club99,
I tried the honey on some Pan cyan. mycelium.
After about 48 hrs. it started to bubble a little. Is this normal? I brew my own beer and I believe it's fermenting.
I'm scared to smell it, because it might be fermenting, and mead is not what I'm trying to make this time.


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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90370 - 11/16/99 11:33 AM (22 years, 10 months ago)

Anyway seems that your solution were contaminated by bacteria.....
Did u sterilyze well the solution?
I use this tek with PF strain but i never saw bubbling......maybe dont't work with pancyan (i don't think this).
But if u brew by yourself use the malt extract tek....
The reason for wich i use honey is that here in europe home brew isn't common...so i cant find malt.....i'm looking for a tek with very common ingredients.....

Let me know....

-Peace-

[This message has been edited by CLuB99 (edited January 22, 2001).]



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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90371 - 11/16/99 05:16 PM (22 years, 10 months ago)

Also...be sure to don't put too much honey...
You need a very little quantity....not a full teaspoon....just let the honey cover the spoon.......


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Offlinephree_1
Stranger
Registered: 06/03/01
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Last seen: 21 years, 3 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90372 - 11/17/99 02:46 AM (22 years, 10 months ago)

Club99,
I sterlized the solution. I smelled the jar and it smells like honey and shrooms. I don't believe it's contaminated.
I also think that if spores will grow on it so will mycelium. It was just a experiment with some left over mycelium. I am a frim believer in experirmenting, it's a excellent way to learn.
I am a all grain brewer. I don't use malt extract. If you need some malt extract I can tell you were to get it delivered from the USA.


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Offlinephree_1
Stranger
Registered: 06/03/01
Posts: 64
Last seen: 21 years, 3 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90373 - 11/17/99 02:47 AM (22 years, 10 months ago)

Club99,
I will grow some out and see what happens.

[This message has been edited by phree_1 (edited November 16, 1999).]



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OfflinePanTrop
enthusiast
Registered: 11/03/99
Posts: 288
Loc: Washington
Last seen: 20 years, 7 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90374 - 11/20/99 05:44 AM (22 years, 10 months ago)

Honey?!?!? WOW I would have never thought of that! Well, I know that mushroommagic.com sells what they call LCIP (Liquid Culture Inoculum Powder) basically you add 2 cups distilled water (mineral or any water purified will do) and add 5 and a 1/2teaspoons of the LCIP to a 1 pint regular mouth jar (they say regular mouth because they send you regular mouth filter disks) sterilize your typical method via pressure cooker or boiling water in a pot wait till its cooled to inoculate. Its sapose to start growth of mycelium in 3-7 days then wait till the jar has been colonized 50% and then inculate your substrate directly. I have not tried this yet, But am intrested in trying. If anyone else does this or has used the LCIP please post results....I will do the same

------------------
"My mind dont work if my spine dont jerk"
-Quote from Eminem-

~PanTrop~



--------------------
" My mind dont work if my spine dont jerk!"


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OfflineWahoo
Cap'n
Registered: 06/15/99
Posts: 32
Loc: right behind you
Last seen: 11 years, 6 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90375 - 11/20/99 07:16 PM (22 years, 10 months ago)

I've used LCIP from Mushroom Magic. Not bad. Though, i have my suspicions that the contents of the their LCIP is just malt. Nice light yellow colored powder. Maybe theres something else in it, but i don't know. Now, when you're done mixing it, you have a kinda brown liquid. It kinda lightens up after autoclaving uinless you "burn" it. Then it gets darker, as you've carmalized some sugars in the malt. You avaoid burning it by not putting your burner on high when getting up to temp/pressure. As mycelium runs through the liquid, it gets lighter a little. I've had really bad luck innoculating broth from spore syringes. I'd suggest a wedge of agar instead. Maybe its just me and my bad luck, but nothing ever contaminates until i innoculate, then everything goes to hell. But I'm pretty sure a pure culture from agar is a much better way to go about things, as broth is far less forgiving than rice and birdseed. Either that or I've just had some nasty luck with the syringes i get delivered...though I'm pretty damn sure some brownish gunk is living in the Pan Cyan syringe i just got in the mail... ugh.


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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90376 - 11/21/99 04:10 AM (22 years, 10 months ago)

club99 or anyone else,
How long do I need to sterilize the teaspoon of honey and half pint of spring water. Unless I missed it, noone has mentioned it. By the way, that was a great idea, I have tried a few liquid mycelium teks only to meet with failure. If you answer this tonight you bet tommorow I will rise nice and early to try it.

------------------
Sometimes you get shown the light in the strangest of places if you look at it right.



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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90377 - 11/21/99 02:02 PM (22 years, 10 months ago)

You dont need very long times...20-30 min. it's enough in a pressure cooker...
I put the cooker on the fire then i wait until starts boiling...then i count the time......

------------------------------
Hey Moe!!!
Thanks
------------------------------



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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90378 - 11/22/99 06:57 AM (22 years, 10 months ago)

Club99 - Sometimes it seems that the best ideas are the most simple ones, and it takes someone with creativity and imagination to show you what should have been obvious. Wonderful idea! Anyway, I was wondering if it would be possible to drop a small piece of a fine fresh specimen into the honey water and have it grow out into mycelium? I have heard that this works on agar and is the best way to clone a particular specimen. Just a thought, maybe a good way to grow out a single strain of mycelium water.

------------------
Peace and Love, Baby!



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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90379 - 11/22/99 02:58 PM (22 years, 10 months ago)

Saturday 8:00 pm: time of injecting liquid mycelium
Sunday 10:00 pm: i can see mycelium starting grow in jar !!!!!!!! :biggrin: :biggrin: :biggrin:


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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90380 - 11/22/99 04:33 PM (22 years, 10 months ago)

Yesterday I injected 4 cc's of the PF strain spore solution into a half pint jar with regular tap water, and regular honey. I am blessed with a first use very clean water tap water, so I don't have any concerns about that.

But then I reread the orginal post and it mentioned yellow honey from the health food store. What kind of honey is that? I know there are different types of honey, but would it affect what is trying to be accomplished here?

I don't know if mine is working yet, I will check it when I get home tonight.

Let me know if it is some type of special honey that is needed, this is very interesting.

------------------
Sometimes you get shown the light in the strangest of places if you look at it right.



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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90381 - 11/22/99 09:14 PM (22 years, 10 months ago)

Uriah Heep:
I think (maybe)it should work: If you look for the 9er tek (http://www.shroomery.org/9er/index.html) he clones the mushs using the water (made blending a piece of shroom) to inject new jars....but it's not important how is the medium(solid or liquid)...the spores and the myc look for nutrients and good climate conditions.....when they found they'll start to grow....in the other hand you can get your solution moldy.... :frown:
This is why the piece of mush isn't able to extends ryzos (really i'm not sure of this but...i'll try and i'll post results here..)

Foster:
Because with honey buyed at health food store i'm sure that in the honey there's nothing but honey (no preservatives,antimycotics etc.)..the same as for the brown rice flour....



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Offlinejoee
journeyman
Registered: 04/07/99
Posts: 15
Last seen: 21 years, 6 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90382 - 11/24/99 03:19 PM (22 years, 10 months ago)

thinkin of trying...
any more sucess stories?
will the honey carmelize if cooked too long like malt?


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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90383 - 12/20/99 09:44 PM (22 years, 9 months ago)

I injected two jars (250ml) filled with honey/water mix with my p. cubensis spore syringe. Now I?am waiting for mycelium growth again.


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Invisiblevivid
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90384 - 12/21/99 08:37 AM (22 years, 9 months ago)

I recently tried this, but put a little *more* honey in, cause it was really late, and I was tired. Anyway, It looked like I got some debris floating around in it once i sterilized. Do you think any of this would really make a difference?

vivid

[This message has been edited by vivid (edited December 21, 1999).]



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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90385 - 12/21/99 03:27 PM (22 years, 9 months ago)

I?m not 100% sure, but I think that 19 hours after injecting there is some mycelium growing in my jars!!!!


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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90386 - 04/10/00 01:31 AM (22 years, 5 months ago)

Wow! Congratulations to CLuB99, the inventor of the honey water tek! Great job, this will make things a lot easier for some of us. Once again, well done!

------------------

What little I know....



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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90387 - 04/10/00 02:41 AM (22 years, 5 months ago)

Excellent job!!! :smile: I think I'll be trying this new tech out now. It would be so much more efficient and less time consuming if it works.

------------------
Trying to rationalize with an irrational person, is in itself irrational!



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Offline3Mshroom
old hand

Registered: 04/03/00
Posts: 497
Last seen: 21 years, 10 days
Re: Mycelium growing into a liquid media [Re: sutec]
    #90388 - 04/10/00 03:33 AM (22 years, 5 months ago)

T^his tek definately works but I suspect that the credit belongs to hippie3. As an added note though, you have to be careful not to use to much honey. It contains hydrogen peroxide and to much can kill your spores.
Once the spores have sprouted, you can add a shot of peroxide then. The bubbling action of the reaction will cause the mycelium to float to the surface. Makes it easier to concentrate the mycelium in a syringe.

------------------
Be sure to check out www.3mshrooms.homestead.com for your spore needs



--------------------
Corruptissima republicae, plurimae leges
Illegitumus non carborundum


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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90389 - 04/10/00 03:04 PM (22 years, 5 months ago)

quote:
Originally posted by 3Mshroom:
This tek definately works but I suspect that the credit belongs to hippie3.

Really i don't think so! just see the date of this post and you can see the credits of this tek.....

Dr. Lindeman.....thanks! :smile:

BTW i'm building some pages with the honeytek as well as some other useful tips for the beginner.....



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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90390 - 04/10/00 08:01 PM (22 years, 5 months ago)

CLuB99, Credit where credit is due. When I saw your post, I informed everyone on ADM about your breakthrough, and I got flamed by hip, check ADM archive. Perhaps he added a slice of lime to it. Anyway, great job buddy! Look forward to your next post!!!

------------------

What little I know....



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InvisibleHippie3
mycotopiate
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Registered: 11/07/99
Posts: 3,090
Loc: mycotopia.net
Re: Mycelium growing into a liquid media [Re: sutec]
    #90391 - 04/11/00 06:57 PM (22 years, 5 months ago)

what a crock.
yes, cluB99 tried honey befor i did.
but look at the dates in this thread.
there was no cluB99 honey tek.
this thread wa dead until doc charlie, playing ego games i suppose out of jealousy, resurrected it.
i posted the Honey Tek to adm, not cluB99 nor doc.
i made it popular and if it were not or my efforts, the idea would have stayed buried in the vaults.
so give the devil his due. or do a search here for cluB99's honey tek if you doubt because there ain't one!


--------------------
Admin @ mycotopia.net
Mycotopia


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OfflineTriGuy
Skeptic

Registered: 08/28/99
Posts: 105
Loc: Georgia
Last seen: 20 years, 28 days
Re: Mycelium growing into a liquid media [Re: sutec]
    #90392 - 04/21/00 01:15 AM (22 years, 5 months ago)

So, after all this discussion, the wrap up is, "Where is the "Spore to Liquid Media to Injecting Mycelium Tek" ? This is the one that has been successfully repeated by another shroomer on his own. It does not matter who get's the credit since each person usually makes minor adaptations as they perfect for themselves in their environment.

For example: When doing the http://www.shroomery.org/findorgrowthem.php?View=docs&doc=36
it is important to know that Shroom_Wizard lives on the Florida coast, one of the highest humidity climates in this country.



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OfflineWildLASER
Infinity In AGrain Of Salt

Registered: 04/02/00
Posts: 870
Last seen: 2 years, 9 months
Re: Mycelium growing into a liquid media [Re: sutec]
    #90393 - 04/23/00 10:34 PM (22 years, 5 months ago)

I just got some Pure (Clover) Honey from Dutch Farms brand, is this ok? It says PURE HONEY.


--------------------
,'~Þ


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InvisibleHippie3
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90394 - 04/24/00 12:52 AM (22 years, 5 months ago)

sounds like the right stuff.
as long as it's free of preservatives and contains no honeycomb.

------------------
Visit http://www.drooldonkey.org Global Psychedelic Community, Forums & Chat, Teks & Tips.



--------------------
Admin @ mycotopia.net
Mycotopia


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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90395 - 04/24/00 01:56 PM (22 years, 5 months ago)

I have been using the honey tek for about a month
and have only great this to say about it
No contam's and fast growth.
The only differance is that I transfer 1/10th
of a petri plate of mycelium to 250 ml
solution. I have about 10 seperate strains
growing and there all doing great.
Today I am doing a mass spore injection
1 print of tasmainian dark and thick to
250 ml of honey water.
let you all know how it goes.


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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90396 - 05/10/00 01:25 PM (22 years, 4 months ago)

Ok guys...
Two links to the honeytek.... http://www.piratecity.com/substances/club99/Honeytek.html http://novenove.homepage.com/Honeytek.html
Also i emailed the page to aphex....

------------------
Please visit
novenove.homepage.com



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Anonymous

Re: Mycelium growing into a liquid media [Re: sutec]
    #90397 - 05/10/00 05:18 PM (22 years, 4 months ago)

Would it be possible to just boil the jar (say for an hour) in a saucepan instead of using a pressure cooker? Can't afford one.


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InvisibleCLuB99
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Re: Mycelium growing into a liquid media [Re: sutec]
    #90398 - 05/10/00 05:29 PM (22 years, 4 months ago)

Really i don't know...i have a pressure cooker so i never tried...

------------------
Please visit
novenove.homepage.com



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