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OfflineDamion5050
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Re: Agar poured petri condensation question... [Re: Damion5050]
    #9032131 - 10/05/08 12:10 PM (15 years, 4 months ago)

Also how much MS should I put on a single dish  ??


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Offlinedenger
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Registered: 09/19/08
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Re: Agar poured petri condensation question... [Re: RogerRabbit]
    #9032196 - 10/05/08 12:28 PM (15 years, 4 months ago)

Quote:

RogerRabbit said:
Even with my flowhood and putting back in the sleeve, they'll rarely last more than a month before spots of green mold begin to show up. 





RR: My guess you are not sealing them individually?
Try paraffilm or Saran Wrap.
When they are not sealed individually, simple temp. fluctuations cause significant air exchange, which can bring in mold spores. I do get an occasional growth on a dish that was poured over a month ago, but thats more of a rare exception then the rule.


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OfflineRogerRabbitM
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Re: Agar poured petri condensation question... [Re: denger]
    #9032296 - 10/05/08 12:49 PM (15 years, 4 months ago)

I'm aware of all that.  I save the parafilm for after inoculation.  You don't need to save dishes longer than a month anyway.  Petri dishes are for the quick 'isolate and transfer' work, not expanding mycelium or long term storage.
RR


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