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Offlinekleaver
myco-clown

Registered: 07/01/08
Posts: 91
Last seen: 16 years, 1 month
dry verm layer
    #9029355 - 10/04/08 04:43 PM (16 years, 3 months ago)

this is half contam half preparation technique so i decided it's better suited for here.

i finally got my hands on some ball jars and put holes in the lids for innoc. had some trouble with contam which i've never had before. it's weird. ive been sterilizing, then opening to put dry verm layer on bc i dont want it to get damp during sterilization/steaming, and then closing back up and setting in incubator.

could this be my problem?  or... ive been thinking i had the incubator which is not airtight under my bed which is filthy and probably swarming with weird contam. so i used clorox wipes inside the incubator and i'm putting it up on a dresser.

it could just be the exposure to contam... but i gues MY QUESTION IS... for the lidded ball jars, which i am new at using, do you NEED a dry verm layer? if so, when do you add it? do you open the lid at all after sterilization (to put dry verm in)...
im used to just open mouthed 2.5-3oz cups with which i never had a single contam problem.


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"The secret to creativity is knowing how to hide your sources."
-Albert Einstein

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OfflinejustAdream
Stranger
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Registered: 09/28/08
Posts: 613
Loc: northern , CO
Last seen: 14 years, 5 months
Re: dry verm layer [Re: kleaver]
    #9029360 - 10/04/08 04:44 PM (16 years, 3 months ago)

deleted


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cloning tek

:cubie: :cubie::cubie: :getstoned: :cubie::cubie: :cubie:

Edited by justAdream (10/04/08 05:30 PM)

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Offlinegreystealth
Corrupt Cop


Registered: 11/09/07
Posts: 985
Last seen: 14 years, 3 months
Re: dry verm layer [Re: kleaver]
    #9029369 - 10/04/08 04:48 PM (16 years, 3 months ago)

Quote:

kleaver said:
this is half contam half preparation technique so i decided it's better suited for here.

i finally got my hands on some ball jars and put holes in the lids for innoc. had some trouble with contam which i've never had before. it's weird. ive been sterilizing, then opening to put dry verm layer on bc i dont want it to get damp during sterilization/steaming, and then closing back up and setting in incubator.

could this be my problem?  or... ive been thinking i had the incubator which is not airtight under my bed which is filthy and probably swarming with weird contam. so i used clorox wipes inside the incubator and i'm putting it up on a dresser.

it could just be the exposure to contam... but i gues MY QUESTION IS... for the lidded ball jars, which i am new at using, do you NEED a dry verm layer? if so, when do you add it? do you open the lid at all after sterilization (to put dry verm in)...
im used to just open mouthed 2.5-3oz cups with which i never had a single contam problem.




You're supposed to put the dry verm layer before sterilization.

The reason is, because you don't want to open that lid after sterilization, as thousands of new contams will go inside the moment you open that lid. Once your jars are sterilized, never open the lids until they are completely colonized. That's the rule.


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Offlinekleaver
myco-clown

Registered: 07/01/08
Posts: 91
Last seen: 16 years, 1 month
Re: dry verm layer [Re: justAdream]
    #9029371 - 10/04/08 04:48 PM (16 years, 3 months ago)

wow. thank you for wasting the thirty seconds of my life typing this up...

you are helping no one by responding to a thread and saying nothing. get a life and stop scrounging around messageboards all day looking for something to respond negatively to


--------------------
"The secret to creativity is knowing how to hide your sources."
-Albert Einstein

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Offlinekleaver
myco-clown

Registered: 07/01/08
Posts: 91
Last seen: 16 years, 1 month
Re: dry verm layer [Re: kleaver]
    #9029388 - 10/04/08 04:52 PM (16 years, 3 months ago)

cool. ty stealth....

the only thing that threw me off is i used to use open mouth lids... i'd sterilize with tinfoil... then take the tinfoil off, put some more dry verm in because the sub shrinks a little... and leave the tinfoil off for more FAE...

and i never had contam. idk if i was lucky? idk


--------------------
"The secret to creativity is knowing how to hide your sources."
-Albert Einstein

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Offlinegreystealth
Corrupt Cop


Registered: 11/09/07
Posts: 985
Last seen: 14 years, 3 months
Re: dry verm layer [Re: kleaver]
    #9029403 - 10/04/08 04:58 PM (16 years, 3 months ago)

yeah, you were lucky.

you can use tinfoil also, but you're definitely not supposed to take it off without any barriers.

As far as barriers go, the more, the better.

My wife uses a dry verm layer, as well as stuffed cotton inside the inocculation hole for added security.


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Offlinedead
grateful
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Registered: 08/28/08
Posts: 2,761
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Re: dry verm layer [Re: kleaver]
    #9029420 - 10/04/08 05:01 PM (16 years, 3 months ago)

You said you put "some more dry verm" which indicates you already had some dry verm there... that's why you didn't get contams, the dry verm you had protected you. IMO it doesn't matter if the substrate shrinks.


--------------------
"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."

Links:
:regularshroom:Nibin's Guide for Noobs
:regularshroom:some easy teks on bulk & grain prep. (my journal)

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Offlineray40cal
omnitrippint
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Registered: 05/05/08
Posts: 1,308
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Re: dry verm layer [Re: dead]
    #9029429 - 10/04/08 05:03 PM (16 years, 3 months ago)

Leave a half inch of space for the dry verm layer, wipe the mix off the lip of the jar, put the dry verm in to the top.  Use foil to seal off steam intrusion into your jar, it will keep the dry verm dry. Don't put the jars under your bed where it's nasty, put them on a shelf or something. It does not matter one bit if light touches your colonizing jars, I promise you.


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Offlineveda_sticks
Cultivator
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Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
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Last seen: 5 years, 14 days
Re: dry verm layer [Re: ray40cal]
    #9029670 - 10/04/08 06:49 PM (16 years, 3 months ago)

dont change the teks. the pf tek has been proven to work. if your dry verm layer is getting moist during pc then water is getting in. which can be caused by not covering the tops of your jars.                                     

adding the dry verm layer afterwords will just risk contamination. how did you add the dry verm layer? did you strilise the verm?


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinecmod777debian
The Flood


Registered: 07/11/08
Posts: 607
Last seen: 11 years, 10 months
Re: dry verm layer [Re: veda_sticks]
    #9029673 - 10/04/08 06:52 PM (16 years, 3 months ago)

put in the BRF and verm mixture

put dry later on top

cover with foil

inoculate

incubate for 2 days

remove foil.........let those fuckers breathe


--------------------
Something like everything.  Nothing like anything

I think for myself....therefore I am myself

I look both ways before crossing the street, 4 times.  It's not because I am affraid to get hit by a car, it would just be a real inconvenience.

Don't make a wish.  Make a decision

My idea of gun control is a firm grip!


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Offlinekleaver
myco-clown

Registered: 07/01/08
Posts: 91
Last seen: 16 years, 1 month
Re: dry verm layer [Re: cmod777debian]
    #9043135 - 10/07/08 03:58 PM (16 years, 3 months ago)

yea. my method was for the open cups.....

brf mix, dry verm layer, cover with foil and cook

then inoc. and add more dry verm to the top of the cup because of sub. shrinkage

just set and forget


this last batch that i did, and i will keep updated with the results... process was..... fill up, with verm layer, and screw lids on.  cover in foil, cook, remove foil and inoc, set and forget


--------------------
"The secret to creativity is knowing how to hide your sources."
-Albert Einstein

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Offlineveda_sticks
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Folding@home Statistics
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Re: dry verm layer [Re: kleaver]
    #9043972 - 10/07/08 06:22 PM (16 years, 3 months ago)

ight shrinkage of the sub wont make any difference to
the dry verm layer. it will redistribute itself. 

    also dont forget to wipe the inside lip of the jar clean. you dont want any brf on that part otherwise contaminants could grow down the sides.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinekleaver
myco-clown

Registered: 07/01/08
Posts: 91
Last seen: 16 years, 1 month
Re: dry verm layer [Re: veda_sticks]
    #9086420 - 10/16/08 12:40 PM (16 years, 3 months ago)

getting that incubator off the floor was the key. no contam. just the occasional case of a blank shot, where either i got no spores in the jar, or maybe a poor mixture of brf to verm.... either way. most are colonized with no contam. it's nice.

i have always used brf cakes. slightly nervous about starting my next project bc ill be using wbs. it just makes mroe sense for mixing with bulk.  instead of putting the cakes in a baggie and smashing them like i do. haha.


--------------------
"The secret to creativity is knowing how to hide your sources."
-Albert Einstein

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