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InvisibleCDS
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Agar poured petri condensation question...
    #9027729 - 10/04/08 10:05 AM (15 years, 5 months ago)

Ok, so I poured some agar, and after like 8 hours of sitting in room temperature, all the condensation was finally gone... Then I put them in the fridge; and now they are condensated right back up again! I know the condensation is from a change in temperature, but my question is:

Will they eventually reabsorb the condensation while in the fridge, or will I have to take them back out and let them warm up first?

-Thanks

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OfflineRogerRabbitM
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Re: Agar poured petri condensation question... [Re: CDS]
    #9027812 - 10/04/08 10:44 AM (15 years, 5 months ago)

The moisture holding capacity of the agar is dependent on the temperature.  When you put them in the refrigerator, you lowered the ability of the agar to hold the moisture, thus it puked it out.  I don't recommend putting petri dishes in the fridge.  Use master culture slants in test tubes for long term storage.  Keep petri dishes at room temp.
RR


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Offlineveda_sticks
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Re: Agar poured petri condensation question... [Re: RogerRabbit]
    #9029727 - 10/04/08 07:10 PM (15 years, 5 months ago)

another little usefull snipet of information, noted


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
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Franks Spawning To Bulk - Monotub
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Invisiblelipa


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Re: Agar poured petri condensation question... [Re: CDS]
    #9029792 - 10/04/08 07:33 PM (15 years, 5 months ago)

If you have to put petri dishes in the fridge insulate them. Cover the dishes with 2 layers of aluminum foil and you will see a noticeable difference. Don't adjust the temperature of the fridge after inserting cultures or plates into it either. Check the temperature ahead of time.

No they won't reabsorb it.

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OfflineJuke Adro
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Re: Agar poured petri condensation question... [Re: lipa]
    #9029877 - 10/04/08 08:04 PM (15 years, 5 months ago)

I had this problem last night one dish (the top one) was at room temp which is very cold ATM so I changed the temp the petri vomited out so much moisture I could have had a drink, I had to open it up and empty in a sterile condition (that's not recommended) but it will drown your culture if too much has built up, maybe if it gets bad you can do the same.


--------------------
Someone said:  im actually not using ms, im using prints.
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OfflineDamion5050
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Re: Agar poured petri condensation question... [Re: Juke Adro]
    #9030312 - 10/04/08 10:14 PM (15 years, 5 months ago)

Same thing happen to me last night I made up 15 petri dishes for the first time ever..  How long are the dishes good for at room temp with no culture in them ?? I can't find a tek for making long term culture slants.. Anyone got a link??

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OfflineJuke Adro
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Re: Agar poured petri condensation question... [Re: Damion5050]
    #9030515 - 10/04/08 11:07 PM (15 years, 5 months ago)

Yeah RR slants


--------------------
Someone said:  im actually not using ms, im using prints.
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InvisibleCDS
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Re: Agar poured petri condensation question... [Re: Damion5050]
    #9030683 - 10/04/08 11:57 PM (15 years, 5 months ago)

Quote:

Damion5050 said:
How long are the dishes good for at room temp with no culture in them ??




What he said...

So I will take them out of the fridge when I get home, but will they reabsorb the moisture or did I permanently damage them???

(I put them in the fridge because I didn't want any contamination to be able to sneak in and start growing and I also didn't want to waste parafilm on uninoculated petris. I assume it's harder for contams to grow in cooler temps...)

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Invisibletripchip
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Re: Agar poured petri condensation question... [Re: CDS]
    #9031056 - 10/05/08 02:23 AM (15 years, 5 months ago)

how long are the dishes good for with no culture in them at room temp?  been wondering this myself since i have 20 of them ready to go for the next transfer. been sitting for 10 days maybe.. none show signs of contams. I even have 1 sitting out in the open (with parafilm around the sides) to see how long it can just sit for untill i see contams. If i have to move it i just toss it around. I dont try to be gentle with it. just seeing how long it takes till it looks bad or grows contams. the ones waiting to be used are in a tupperware on the floor. they are still in the original sleeve and I sprayed the tupperware down with lysol before storing them in there so they are pretty much in a very clean environment. I been wondering how long they'll stay good for. I peek in there every few days but then squirt more lysol inside the tupperware before I close it. I call it "my clean box" where I store things that need to remain clean, like petri dishes.. and pretty much petri dishes only. :wink:

so yeah, anyone got experience on how long they stay fresh for?


--------------------
Psilocybe Cubensis

Psilocybe comes from the Greek root "psilos" meaning bald head and Cubensis because it was first recognized as a new species from specimens collected in Cuba.

                                    :mushroom2:CHIP:mushroom2:

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Invisiblelipa


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Re: Agar poured petri condensation question... [Re: tripchip]
    #9031571 - 10/05/08 08:52 AM (15 years, 5 months ago)

I store my blank dishes by wrapping with parafilm or plastic wrap, placing into a fresh bag, tie the bag off , and then place into my still air box. I make up about 50-100 at a time and use them over the course of weeks. I see no need to refrigerate them.

When I need one I open the bag up, take one out, wipe outside with isopropyl alcohol, remove the wrap and inoculate. Works 99 % of the time. I have used 4 week old petri dishes many of times with no failure.

Edited by lipa (10/05/08 08:59 AM)

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Offlineveda_sticks
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Re: Agar poured petri condensation question... [Re: lipa]
    #9031586 - 10/05/08 08:57 AM (15 years, 5 months ago)

Nice still air box. When i start growing again i will be making something like that.

To those that complain that gloveboxs are awkword, not if you have one like his


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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InvisibleCDS
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Re: Agar poured petri condensation question... [Re: veda_sticks]
    #9031600 - 10/05/08 09:06 AM (15 years, 5 months ago)

Hey, how'd you cut those arm holes? They look super smooth...

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Offlineveda_sticks
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Re: Agar poured petri condensation question... [Re: CDS]
    #9031608 - 10/05/08 09:09 AM (15 years, 5 months ago)

Probably one of those drill bits that you get for cutting large holes in wood. the ones that are like a round saw.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisiblelipa


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Re: Agar poured petri condensation question... [Re: CDS]
    #9031611 - 10/05/08 09:10 AM (15 years, 5 months ago)

I program NC code and CAD for a job. I have 2 CNC at my disposal.

I am getting ready to start making prefab still air boxes and flow hood kits to save you guys some money and time. I will probably  start advertising here when I get finished with preparation.

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Offlineveda_sticks
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Re: Agar poured petri condensation question... [Re: lipa]
    #9031615 - 10/05/08 09:12 AM (15 years, 5 months ago)

I just noticed the ziplock bags covering the arm holes.

Its a teky getto still air box

A:hotidea:


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisiblelipa


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Re: Agar poured petri condensation question... [Re: veda_sticks]
    #9031617 - 10/05/08 09:14 AM (15 years, 5 months ago)

People here go way overboard with the still air shit. I used this box for a long time before I made my hood. I had very good succes with it.

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Offlineveda_sticks
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Re: Agar poured petri condensation question... [Re: lipa]
    #9031623 - 10/05/08 09:18 AM (15 years, 5 months ago)

Yeah, mine was just a storage container that had a lid ontop that folds open in 2 peices with badly cut arm holes. 100% success rate with it. 90% failure rate without it.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinedenger
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Re: Agar poured petri condensation question... [Re: Damion5050]
    #9031657 - 10/05/08 09:39 AM (15 years, 5 months ago)

Quote:

Damion5050 said:
How long are the dishes good for at room temp with no culture in them ??




They are good for as long as there is no growth on them and they do not dry out. I have used dishes poured two months earlier with identical results to the fresh ones.
Here is what I do: I wrap the perimeter of the dish with plastic and mark the pour date on the lid. There are a couple of types of plastic I use: paraffilm (lab film), but its $$, or narrow rolls of Saran Wrap from the grocery store. Wow, hold on a sec, the grocery store sells rolls of Saran Wrap which are almost a foot wide?!! Yes, I cut them with a sharp bread knife or with electric carving knife into small 1/2 - 3/4 inch wide rolls. These little rolls are super-handy for wrapping dishes, either empty ones for storage or the ones with the cultures.

Quote:

I can't find a tek for making long term culture slants.. Anyone got a link??




Stamets describes slants in GGMM. Simply put, its a test tube (approx. 20x125 mm usually, but more or less will work), filled 1/3 with agar media, sterilized and cooled sitting on a shallow angle so that it solidifies as a wedge. Once colonized with mycelia, it is stored in the fridge.
There is a simpler tek:
http://www.shroomery.org/8509/Resurrecting-a-Better-Method-for-Long-Term-Storage-of-Mushroom-Cultures

Also, see this thread:
Storing cultures in mineral oil ?

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OfflineRogerRabbitM
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Re: Agar poured petri condensation question... [Re: Damion5050]
    #9031800 - 10/05/08 10:48 AM (15 years, 5 months ago)

Quote:

Damion5050 said:
How long are the dishes good for at room temp with no culture in them ??




I slip them back into the sleeve they came packaged in once they cool down.

Even with my flowhood and putting back in the sleeve, they'll rarely last more than a month before spots of green mold begin to show up.  However, if you're doing a series of strain isolations, you'll use up 20 dishes long before that time.

The thing to remember is not to try to make too much at a time.  A bit over 1/2 liter is about right to pour 20 dishes.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineDamion5050
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Re: Agar poured petri condensation question... [Re: RogerRabbit]
    #9031888 - 10/05/08 11:09 AM (15 years, 5 months ago)

Yeah I did 500ml and 25 grams of PDA per the instructions on the bottle from myco supply and I was able to pour 15 dishes.. I could of got 20 but I poured a little to much into a couple lol...

I basically plan on using the agar with MS syringes and trying to make sure there is no contams in the syringes then turn a agar wedge into LC..  Then I will proceed to fruit the LC the isolate/Clone a good performing shroom on another agar dish..

Does that sound O.K.

Edited by Damion5050 (10/05/08 11:27 AM)

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OfflineDamion5050
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Re: Agar poured petri condensation question... [Re: Damion5050]
    #9032131 - 10/05/08 12:10 PM (15 years, 5 months ago)

Also how much MS should I put on a single dish  ??

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Offlinedenger
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Re: Agar poured petri condensation question... [Re: RogerRabbit]
    #9032196 - 10/05/08 12:28 PM (15 years, 5 months ago)

Quote:

RogerRabbit said:
Even with my flowhood and putting back in the sleeve, they'll rarely last more than a month before spots of green mold begin to show up. 





RR: My guess you are not sealing them individually?
Try paraffilm or Saran Wrap.
When they are not sealed individually, simple temp. fluctuations cause significant air exchange, which can bring in mold spores. I do get an occasional growth on a dish that was poured over a month ago, but thats more of a rare exception then the rule.

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Re: Agar poured petri condensation question... [Re: denger]
    #9032296 - 10/05/08 12:49 PM (15 years, 5 months ago)

I'm aware of all that.  I save the parafilm for after inoculation.  You don't need to save dishes longer than a month anyway.  Petri dishes are for the quick 'isolate and transfer' work, not expanding mycelium or long term storage.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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