Home | Community | Message Board


Myco Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar

Jump to first unread post. Pages: 1
Offlineellisdee625
enthusiast
Registered: 03/28/02
Posts: 271
Last seen: 14 years, 7 months
clone to agar
    #902638 - 09/23/02 09:38 PM (14 years, 9 months ago)

is it possible to clone from a mushy to agar? i've done multispore and mycilium transfer, but never cloned to one. just curious. i want the characteristics of this particular mushy, but want to see if i can isolate it further to maybe enhanse it a little more. thanks


--------------------
Have a smurfy day!!!


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleTripsAreForKids
addict

Registered: 04/23/01
Posts: 465
Loc: Not In The US
Re: clone to agar [Re: ellisdee625]
    #902658 - 09/23/02 09:45 PM (14 years, 9 months ago)

yea sure...

Just take the fresh mushrooms, cut the stem into a cylinder, use a razor to peel back layers around the stem like a banana, to get the fresh sterile (semisterile) inner stem portion, and toss it on the agar.


--------------------
Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 23 days, 14 hours
Re: clone to agar [Re: ellisdee625]
    #902669 - 09/23/02 09:48 PM (14 years, 9 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Offlineellisdee625
enthusiast
Registered: 03/28/02
Posts: 271
Last seen: 14 years, 7 months
Re: clone to agar [Re: TripsAreForKids]
    #902671 - 09/23/02 09:49 PM (14 years, 9 months ago)

i'll start immediately. thanks


--------------------
Have a smurfy day!!!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: clone to agar [Re: ellisdee625]
    #902734 - 09/23/02 10:13 PM (14 years, 9 months ago)

In fact you may not even have to select the best mycelium (like when you did multi) , that is advantage to doing clones , but it is reccomended that you return to spore/agar if any signs of degredation shows up . That is if strong rhizmorphic growth becomes hard to obtain from culture . :cool:
Good luck :grin: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMush-e
Male User Gallery

Registered: 04/29/16
Posts: 16
Last seen: 9 months, 27 days
Re: clone to agar [Re: ellisdee625]
    #23261228 - 05/23/16 12:13 PM (1 year, 1 month ago)

hi yes it is, pick your fastest biggest sample if you don't have a flowhood you can use hydrogen peroxide in your agar mix to help with contamination. But you still need to be sterile as possible.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJosex
 User Gallery


Registered: 11/13/15
Posts: 2,846
Loc: Flag
Last seen: 58 minutes, 41 seconds
Re: clone to agar [Re: Mush-e]
    #23261240 - 05/23/16 12:18 PM (1 year, 1 month ago)

Quote:

Mush-e said:
hi yes it is, pick your fastest biggest sample if you don't have a flowhood you can use hydrogen peroxide in your agar mix to help with contamination. But you still need to be sterile as possible.



If you add H2o2 to the agar mix, it will revert back to water during the sterilization run.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,197
Loc: Babylon
Last seen: 12 hours, 31 minutes
Re: clone to agar [Re: Josex]
    #23261328 - 05/23/16 12:43 PM (1 year, 1 month ago)

and both these bumped threads are 10+ yrs old.. none involved are around anymore.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinebodhisattaM
< Click Here >
 User Gallery

Registered: 04/30/13
Posts: 32,546
Last seen: 3 hours, 13 minutes
Trusted Cultivator
Re: clone to agar [Re: Mush-e]
    #23261362 - 05/23/16 12:56 PM (1 year, 1 month ago)

Quote:

Mush-e said:
hi yes it is, pick your fastest biggest sample if you don't have a flowhood you can use hydrogen peroxide in your agar mix to help with contamination. But you still need to be sterile as possible.



:facepalm:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* A couple ?'s re: clones, agar, & casings keyeghost 634 2 02/27/04 02:12 PM
by keyeghost
* Mushroom cloning?? dimitri211 2,367 3 05/23/01 09:49 PM
by Anno
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 all )
agar 27,891 38 12/05/11 11:40 PM
by soicyboy121
* Can I add certain % Peroxide to substrate mix? Numba9 4,452 5 08/23/01 11:31 AM
by shellacct
* Grain to Agar? Aiko Aiko 1,598 12 05/23/16 01:00 PM
by bodhisatta
* cloning...why won't it work for me? carbonhoots 739 9 02/15/02 01:47 AM
by Anno
* Agar PH. Alegalize 607 4 03/30/05 11:42 PM
by Alegalize
* peroxide % ?? growin 424 3 06/08/02 01:52 AM
by growin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
763 topic views. 16 members, 32 guests and 22 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
High Mountain Compost
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.007 seconds on 19 queries.