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bravozulu
Stranger
Registered: 09/25/08
Posts: 1
Last seen: 15 years, 5 months
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Shiitake simple method grown on Aspin chips, wheat bran and gypsom
#8987353 - 09/25/08 11:10 PM (15 years, 5 months ago) |
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This is my first post and is also my first attempt at growing mushrooms. It seems to have worked out very well so I thought I would list my very inexpensive simple method.
Basically I just used a dental pick that was flamed to pick off some tissue from the inside of a shiitake mushroom bought at Trader Joes. I added it to agar in a small jar with cotton stuffed into a small breathing hole. The dental pick also works great at cutting the agar to put it in grain spawn.
I made the potato dextrose agar from some agar I got at mothers market and added the rather concentrated juice from boiling potatoes, some dextrose and a small amount of yeast. I added 2 teaspoons agar, .25 teaspoons sugar and about .1 teaspoon yeast per 100 ml. I then put it in a pressure cooker for 30 minutes. After it cooled and hardened I used a knife cleaned with alcohol and dried to cut up pieces to put them in baby food jars so that it would easily cover the bottom when melted. I then put the extra agar in the fridge and pressure cooked the baby food jars for 30 minutes.
After cooling the jars and the agar hardening, I used the flame sterilize dental pick to cut off a tiny piece of tissue from the inside of a cap I just tore. I made about 20 of them and most were not contaminated.

After a couple of weeks, I used the flame sterilized dental pick to cut out a section of clean mycelium from the jars. I added that to grain spawn made from bird seed. Then I left them is a box for about a month.

To prepare the aspen chips (Kaytee Aspen bedding and liter), I first added some gypsum. I then just boiled it for about 15 minutes and left it to cool and dry somewhat. After about an hour it was ready to mix with the spawn.

I broke up the spawn and mixed in about 1/4 cup of wheat bran. I had many get moldy when I didn't sterilize the bran so I would suggest that in case that was the cause. I also worked out a more efficient method of inoculating the grain spawn with a mycelium culture made by putting some mycelium from the spawn jar into sterilized water and glass chips and shaking it. I borrowed the idea on this site I believe.

Spawn before adding to 3 liter plastic soft drink bottle

What it looks like when done. I found that plastic wrap keeps it humid enough for at least a month when it should be fully colonized.

What it looks like after 4 days.

After about 2 months later. It had spent time spent in fruiting chamber after being exposed to 50 degrees F for a couple of days. After a week these had sprouted.

4 days later (today) it looks like this.

I am wondering if that is how they normally look at this stage?
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combs
Stranger



Registered: 06/10/07
Posts: 219
Last seen: 7 years, 8 months
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Re: Shiitake simple method grown on Aspin chips, wheat bran and gypsom [Re: bravozulu]
#8991254 - 09/26/08 08:38 PM (15 years, 5 months ago) |
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just inspired me to try something similar! I think I'll give blue oysters a shot on a mixture of oak sawdust and maybe some paper or cardbord.
-------------------- All I wanna do is shake jars with you, stay up late at night and make spawn.
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fishfarmin
Predator Hunter


Registered: 09/04/08
Posts: 196
Last seen: 14 years, 10 months
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Re: Shiitake simple method grown on Aspin chips, wheat bran and gypsom [Re: combs]
#8995611 - 09/27/08 07:55 PM (15 years, 5 months ago) |
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would really like to see those other pics.
-------------------- "Government exist to protect us from each other.Where government has gone beyond its limits is in deciding to protect us from ourselves." Ronald Reagan "It is error alone which needs the support of government.Truth can stand by itself."Thomas Jefferson "It has been my experience that folks who have no vices have very few virtues."Abraham Lincoln
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Binder
Bind it on up!


Registered: 09/12/08
Posts: 13
Loc: 北海道
Last seen: 4 years, 9 months
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Re: Shiitake simple method grown on Aspin chips, wheat bran and gypsom [Re: bravozulu]
#8995776 - 09/27/08 08:37 PM (15 years, 5 months ago) |
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A few days ago, you had a picture of them looking all grown up. Where did it go?
However, that picture was definitely hiratake, not shiitake.
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