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OfflineVisigoth
enthusiast

Registered: 11/28/01
Posts: 481
Last seen: 11 years, 2 months
liquid mycelim working, but slower than spores???
    #897762 - 09/21/02 03:44 PM (21 years, 6 months ago)

My friend made a liquid mycelium solution that was looking good. He has been using it to innoculate some birdseed/grain jars. The solution works and has gotten the jars fully colonized, but it takes about 5 or so days to kick in, the spores usually germinate sooner, what's up w. that, any ideas?!?! Thanks!!!!

Vis


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"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: liquid mycelim working, but slower than spores??? [Re: Visigoth]
    #897768 - 09/21/02 03:49 PM (21 years, 6 months ago)

This sounds very unusual. I find that a mycellium innoculation is usually a day or two faster.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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InvisibleGnome69
Cultivator
Registered: 08/26/02
Posts: 146
Loc: My Gnome Abode
Re: liquid mycelim working, but slower than spores??? [Re: Visigoth]
    #897802 - 09/21/02 04:13 PM (21 years, 6 months ago)

Perhaps your friend blended the mycelium too long, thereby destroying the cell structure
or maybe the solution/culture is too old...
I also find mycelium to be faster than spores...at least for oysters....
:crazy: :tongue: :crazy:
:smirk:


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You do not believe, and that is why you fail.

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OfflineVisigoth
enthusiast

Registered: 11/28/01
Posts: 481
Last seen: 11 years, 2 months
Re: liquid mycelim working, but slower than spores??? [Re: Gnome69]
    #897820 - 09/21/02 04:21 PM (21 years, 6 months ago)

Well he didn't use a blender or anything. He used the malt extract solution tek. It's definitely weird. He has noticed that the large majority of the mycelium, the big fat healthy strands, can't be sucked up in the syringe. Any tips on how to make it all suckable?? Hahaha!!! thanks guys!!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: liquid mycelim working, but slower than spores??? [Re: Visigoth]
    #897826 - 09/21/02 04:25 PM (21 years, 6 months ago)

Well, this is your problem. You have a very low concentration of mycellium in the syringe. What I do is suck up and shoot out the water several times. This creates a very forceful stream of water that will quickly break up anything in the solution.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: liquid mycelim working, but slower than spores??? [Re: Visigoth]
    #897827 - 09/21/02 04:25 PM (21 years, 6 months ago)

Throw some broken glass into the jar.....be sure and sterilize the glass.



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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Invisiblemistyblue
Apprentice
Registered: 09/16/02
Posts: 11
Loc: Europe
Re: liquid mycelim working, but slower than spores??? [Re: Roadkill]
    #897915 - 09/21/02 05:15 PM (21 years, 6 months ago)

In reply to:

Throw some broken glass into the jar.....be sure and sterilize the glass.




why?

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: liquid mycelim working, but slower than spores??? [Re: mistyblue]
    #897929 - 09/21/02 05:21 PM (21 years, 6 months ago)

You can use the broken glass to break up the mycelium.....that is in the jar.



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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OfflinePsilosShaman
Strange Famous

Registered: 09/17/02
Posts: 128
Loc: Arkansas...man I love thi...
Last seen: 20 years, 8 months
Re: liquid mycelim working, but slower than spores??? [Re: Roadkill]
    #897955 - 09/21/02 05:34 PM (21 years, 6 months ago)

Broken Glass huh?????  Sounds like a lot of extra, and pointless work.  Sucking the solution up and spraying it back in should do the trick like Mickey said.  And why in the world would you choose broken glass anyway...out of all the dumb things that you could have thought of to throw in the jar,  why would you choose broken glass? :crazy:  No what I mean man :wink: 

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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: PsilosShaman]
    #897973 - 09/21/02 05:42 PM (21 years, 6 months ago)


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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: liquid mycelim working, but slower than spores??? [Re: matts]
    #897976 - 09/21/02 05:44 PM (21 years, 6 months ago)

Yes, and broken glass is inert.  It won't rust into your spore solution... :wink: 


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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OfflinePsilosShaman
Strange Famous

Registered: 09/17/02
Posts: 128
Loc: Arkansas...man I love thi...
Last seen: 20 years, 8 months
Re: liquid mycelim working, but slower than spores??? [Re: mickey_rourke]
    #897991 - 09/21/02 05:51 PM (21 years, 6 months ago)

Well that makes sense then.  I was wondering why you couldn't add something like nuts or bolts.  I guess glass really would be the best bet.  :frown: sorry for posting that without considering the rust factor. 

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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: liquid mycelim working, but slower than spores??? [Re: PsilosShaman]
    #897993 - 09/21/02 05:52 PM (21 years, 6 months ago)

On that note. In the past (before I started doing the syringe trick) I used stainless steel nuts and bolts. Worked perfectly and no rust.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 3 months
Re: liquid mycelim working, but slower than spores [Re: Visigoth]
    #898102 - 09/21/02 06:30 PM (21 years, 6 months ago)

"Any tips on how to make it all suckable??"

Try using a 14 gauge syringe needle, you can get them at a farm suply store. They are much larger and wont clog up as easily.

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