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iMtRiPpIn
Lookin' fer Luv


Registered: 06/04/08
Posts: 530
Last seen: 11 years, 3 months
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Needle Flame and Alcohol
#8957073 - 09/19/08 11:12 PM (15 years, 4 months ago) |
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Is it ok to flame the needle AND wipe it with 90% rubbing alcohol when inoculating multiple jars of grain from a single syringe? Im wondering because Im not getting any growth and Im thinking that maybe the alcohol on the needle killed the spores as they came out? I waited several seconds for it to dry before I shot it out. I flamed, wiped, and shot. Also, I flamed the needle between jars. Is that right? Or do I only need to flame once at the beginning and wipe with alcohol only between jars?
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Krez
RamRod




Registered: 01/08/08
Posts: 1,504
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Re: Needle Flame and Alcohol [Re: iMtRiPpIn]
#8957085 - 09/19/08 11:15 PM (15 years, 4 months ago) |
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Wiping with alcohol does nothing. The first few drops of spore solution shouldve cooled the syringe. Flame the needle in between in inoculation. How long has it been?
-------------------- Kids, you tried your best and you failed miserably. The lesson is, never try. (Homer) Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer) All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)
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slacker008
Resident Psychiatrist


Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
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Re: Needle Flame and Alcohol [Re: Krez]
#8957101 - 09/19/08 11:20 PM (15 years, 4 months ago) |
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Why does alcohol wipes do nothing? I dont like using flame, it leaves my needles charred and discolored. I fill a shotglass with rubbing alcohol and while im doing something else or between inoculations, the needle is in the alcohol glass.
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Krez
RamRod




Registered: 01/08/08
Posts: 1,504
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Re: Needle Flame and Alcohol [Re: slacker008]
#8957129 - 09/19/08 11:24 PM (15 years, 4 months ago) |
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Alcohol doesnt sterilize, it sanitizes.
-------------------- Kids, you tried your best and you failed miserably. The lesson is, never try. (Homer) Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer) All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)
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billyboy36
Stranger
Registered: 05/04/08
Posts: 352
Loc: Canada
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Re: Needle Flame and Alcohol [Re: Krez]
#8957196 - 09/19/08 11:44 PM (15 years, 4 months ago) |
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Quote:
I dont like using flame, it leaves my needles charred and discolored.
That means it's working. Imagine what your mold spores look like. Alcohol doesn't lyse the cells of every bacteria. Red hot is where it's at.
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themange
Senior Citizen



Registered: 06/11/06
Posts: 2,809
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Re: Needle Flame and Alcohol [Re: billyboy36]
#8957203 - 09/19/08 11:46 PM (15 years, 4 months ago) |
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you know whats more sterile than a red hot needle?
nothing.
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LightShedder
Trading currencies



Registered: 08/30/05
Posts: 3,026
Loc: AustinDenverLA
Last seen: 4 years, 7 months
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Re: Needle Flame and Alcohol [Re: themange]
#8957216 - 09/19/08 11:49 PM (15 years, 4 months ago) |
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I always just rub the needle with an alcohol pad with no contaminates to date.
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Krez
RamRod




Registered: 01/08/08
Posts: 1,504
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Re: Needle Flame and Alcohol [Re: LightShedder]
#8957218 - 09/19/08 11:51 PM (15 years, 4 months ago) |
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Then you have been very lucky and do not have a very high mold count in your dwelling.
-------------------- Kids, you tried your best and you failed miserably. The lesson is, never try. (Homer) Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer) All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)
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LightShedder
Trading currencies



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Posts: 3,026
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Re: Needle Flame and Alcohol [Re: Krez]
#8957249 - 09/19/08 11:59 PM (15 years, 4 months ago) |
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still air glove box. and I wouldn't call 0 % contamination out of easily 500 jars luck. I would call it good sterile techniques. And that is still air glove box and a good spray of oust and alcohol pads. I blew myself up one time with a flame in a glovebox because I was being a complete dumbass so since then I am terrified to light a lighter in my still air glovebox even with nothing else in there.
Edited by LightShedder (09/19/08 11:59 PM)
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Krez
RamRod




Registered: 01/08/08
Posts: 1,504
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Re: Needle Flame and Alcohol [Re: LightShedder]
#8957260 - 09/20/08 12:04 AM (15 years, 4 months ago) |
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Some people have really sterile techniques and use glove boxes and still manage to fail. If you blew yourself up (did the hair grow back yet ) why not try putting the alcohol lamp outside the glove box. People are gonna have different results cause there are different factors to consider. Just trying to inform people that using alcohol is just sanitizing not sterilizing.
-------------------- Kids, you tried your best and you failed miserably. The lesson is, never try. (Homer) Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer) All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)
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LightShedder
Trading currencies



Registered: 08/30/05
Posts: 3,026
Loc: AustinDenverLA
Last seen: 4 years, 7 months
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Re: Needle Flame and Alcohol [Re: Krez]
#8957274 - 09/20/08 12:12 AM (15 years, 4 months ago) |
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Yeah I finally have eyebrows again! lol. Yeah I know flame sterilization works great but I just don't agree that "wiping with alcohol does nothing". If you wipe the needle very thoroughly multiple times with 70% alchohol pads in a sterile environment then that is totally effective IME.
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Krez
RamRod




Registered: 01/08/08
Posts: 1,504
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Re: Needle Flame and Alcohol [Re: LightShedder]
#8957328 - 09/20/08 12:36 AM (15 years, 4 months ago) |
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Or in your case a still air environment. Dont get me wrong alcohol is useful as it sanitizes the surface. For inoculations itll work. When you switch over to agar you just can beat flame sterilizing. Im just completely anal about it.
-------------------- Kids, you tried your best and you failed miserably. The lesson is, never try. (Homer) Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer) All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 2 days
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Re: Needle Flame and Alcohol [Re: Krez]
#8957420 - 09/20/08 01:13 AM (15 years, 4 months ago) |
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Alcohol does nothing to kill mold spores INside the hollow needle. Flame until red hot before the first jar and between each jar. Keep the torch outside the glovebox. Use alcohol to wipe the lids or the micropore tape on the lids before inoculations, but not the needle itself. As already said, you can't get more sterile than a red hot needle. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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