Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisibleslacker008
Resident Psychiatrist
 User Gallery

Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Clone to Jars?
    #8948725 - 09/18/08 10:49 AM (15 years, 5 months ago)

Im just harvested a sole fruit from a smaller clump of myc (you can find that in my grow log post) and I am taking not only spore prints, but also using the cardboard cloning technique.

If the clones succeed, I was wondering if I could transfew a peirce or all of the cloned board into a BRF jar? And if so, how?


Extras: Filter Print Post Top
Offlinegrod31
Stranger
Male


Registered: 12/06/07
Posts: 2,077
Loc: New York
Last seen: 15 years, 15 days
Re: Clone to Jars? [Re: slacker008]
    #8948733 - 09/18/08 10:51 AM (15 years, 5 months ago)

if you are  using BRF jars then the only way you can do that is if you make myc laden water with the clone. how else would you not disturb the dry verm barrier


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
Trusted Cultivator
OG Cultivator
Re: Clone to Jars? [Re: grod31]
    #8948755 - 09/18/08 10:55 AM (15 years, 5 months ago)

Quote:

Im just harvested a sole fruit




That's not exactly clone-worthy material there.  Clone a fruit from a giant cluster of healthy, potent fruits, and you'll be onto something.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Offlinebwoy
Stranger
Male User Gallery


Registered: 09/19/06
Posts: 264
Loc: Granada, Andalusia
Last seen: 11 years, 18 days
Re: Clone to Jars? [Re: RogerRabbit]
    #8948772 - 09/18/08 10:59 AM (15 years, 5 months ago)

if you clean a stipe, in clean conditions and strip away the outer surface with a flamed scalpel, you can use a syringe to spear the stipe, taking a small piece of tissue into the end of the syringe, this could then be inserted through the verm barrier.

Stupid but true.




Edited by bwoy (09/18/08 11:00 AM)


Extras: Filter Print Post Top
Invisibleslacker008
Resident Psychiatrist
 User Gallery

Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Re: Clone to Jars? [Re: RogerRabbit]
    #8948798 - 09/18/08 11:05 AM (15 years, 5 months ago)

So the decision is made, then! Clone to cardboard. When colonized, prepare jars and inoculate with myc-water. Anyone know a good tek to make mycelium laden water out of a cardboard clone?

Im so contamination-phobic though. Even cutting up and trying to clone this mushroom has got me freaking out. I keep thinking "No point. Its just going to contam any jar you put it in anyway!"

Oh, and..

Quote:

RogerRabbit said:
Quote:

Im just harvested a sole fruit




That's not exactly clone-worthy material there.  Clone a fruit from a giant cluster of healthy, potent fruits, and you'll be onto something.
RR




http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8944987/an/0/page/0

First picture. If that isnt clone-worthy, I just dont know what is.

For the record, im cloning and printing because I dont want to have to buy another golden teacher syringe!


Edited by slacker008 (09/18/08 11:13 AM)


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
Trusted Cultivator
OG Cultivator
Re: Clone to Jars? [Re: slacker008]
    #8948854 - 09/18/08 11:14 AM (15 years, 5 months ago)

The so-called cardboard tek is vastly over rated.  You'll still need to isolate clean mycelium away from the cardboard before inoculating sterile media.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Invisibleslacker008
Resident Psychiatrist
 User Gallery

Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Re: Clone to Jars? [Re: RogerRabbit]
    #8948872 - 09/18/08 11:18 AM (15 years, 5 months ago)

Quote:

RogerRabbit said:
The so-called cardboard tek is vastly over rated.  You'll still need to isolate clean mycelium away from the cardboard before inoculating sterile media.
RR




This is..that my intention isnt to isolate a certain strain. My only goal here is to propagate and not have to buy more syringes for golden teacher because these are the second ones Ive bought so I want to make sure I dont have to do it again!

Like i said, im cloning to cardboard and spore printing? Its all the same end goal for me.

As far as the clean mycelia and sterile conditions go, I suck at sterile. So I hope I dont ruin a buncha stuff trying to do this! I guess Ill never learn if i dont try!


Edited by slacker008 (09/18/08 11:19 AM)


Extras: Filter Print Post Top
Invisibleseven
.
Male

Registered: 06/09/08
Posts: 1,478
Loc: north carolina
Re: Clone to Jars? [Re: slacker008]
    #8949099 - 09/18/08 12:07 PM (15 years, 5 months ago)

why not clone to agar or a liquid culture? (clone to agar>watch for contams> transfer until you have a clean culture>transfer a piece of the clean culture to liquid culture. when that grows out hopefully you have a contamfree liquid inoculant of your clone. or clone straight to liquid culture and take a risk?


--------------------
grind


Extras: Filter Print Post Top
Invisibleslacker008
Resident Psychiatrist
 User Gallery

Registered: 05/21/08
Posts: 1,103
Loc: A wave of reality.
Re: Clone to Jars? [Re: seven]
    #8949273 - 09/18/08 12:42 PM (15 years, 5 months ago)

I just dont have any agar, honestly! Haha, if I did I would use it. Ive tried liquid culture and didnt like it too much.


Extras: Filter Print Post Top
Invisiblethemange
Senior Citizen
Male


Registered: 06/11/06
Posts: 2,809
Re: Clone to Jars? [Re: slacker008]
    #8949327 - 09/18/08 12:54 PM (15 years, 5 months ago)

since this is in relation to this thread, i thought i would mention it:

i thought I'd try a little experiment and clone a fruit that grew out of a forgotten, completely(or so i thought) dried up blob of substrate i left sitting out in the garage on a table.

i just took a biopsy of it and threw it in some rye, well i have 20quarts colonized now, and although it's not the most rhizomorphic mycelium I've ever seen, all the bags are fully colonized and being spawned.

i guess the idea at the time was that if this sucker could grow in such shitty conditions (outside of the FC with very low/no humidity, completely overlayed, etc.)that when introduced to good growing parameters, it would use it's hardy genetics to thrive.

but then again it may do absolutely nothing.

:shrug:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Jars *DELETED* mdog 606 4 10/15/02 08:13 AM
by DERRAYLD
* Grey/Black in my jars? XiC_clone 988 5 10/12/02 09:06 AM
by Magash
* Temp. of jars Danimal 1,247 5 06/16/02 05:25 PM
by SixTango
* Cloning on a low budget, how's this sound... QuitDrinking 1,258 11 12/25/02 05:49 PM
by Champion des Champignons
* mushrooms growing in cannabis cloning chamber cannabisdreams 1,721 2 07/25/02 03:13 PM
by Killa420
* Innoculating a jar with multiple strains zanchin 3,984 9 03/07/02 01:02 PM
by zanchin
* Honey Water Cloning VIA 9er tek? Moe? Anno? KingCobWeb 1,550 6 02/03/03 10:40 AM
by deanofmean
* Mushroom cloning?? dimitri211 3,955 3 05/23/01 07:49 PM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,222 topic views. 23 members, 170 guests and 51 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.008 seconds on 14 queries.