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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 18 years, 10 months
dont flame me, sterilization questions
    #889086 - 09/17/02 06:25 PM (19 years, 15 days ago)

OK so I read that you gotta PC the jar for 45-60 min. doesnt that sound excessive? does it take that long to kill bacteria and spores? what about ovens or just sitting the jar on a washcloth and sticking it in a pan and let it heat up for 10 min?
if it gets contamunated, isnt it due to inocculating where you have to stick the syringe in the 4 holes on the top?


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889095 - 09/17/02 06:30 PM (19 years, 15 days ago)

not gonna flame...
NOT....GOING...TO..FLAME....
Yes it's all necessary, it takes time for the heat to fully penetrate all of the substrate. Anything less WILL contaminate. If it weren't necessary, trust me, the experts wouldn't do it.


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 18 years, 10 months
Re: dont flame me, sterilization questions [Re: Servator]
    #889125 - 09/17/02 06:39 PM (19 years, 15 days ago)

good answer there. thanks for not flaming me :smile: so hows anger management class going?  :wink: :cool:

one more ?, will the oven do or must a PC be used?


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InvisibleTM
The Mind, The Many, The Music.
Male User Gallery

Folding@home Statistics
Registered: 06/11/02
Posts: 8,282
Loc: Under The Table And Dream...
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889166 - 09/17/02 06:55 PM (19 years, 15 days ago)

*flick!* *flick!* *flick!* ...DAMN!! Lighter's outa butane!! :mad: 


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================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineShaw
hick

Registered: 06/28/01
Posts: 2,220
Loc: midwest, usa
Last seen: 9 months, 14 days
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889187 - 09/17/02 07:04 PM (19 years, 15 days ago)

i'll tell you what i told Z-tard. You don't even have to sterilize them. Just stick your thumb up your ass. Trust me, it helps make them steril.


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889193 - 09/17/02 07:08 PM (19 years, 15 days ago)

Eh, I'm feelin happy today  :tongue:
oven works for pasteurizing bulk substrates, pretty sure it's gotta be pc for jars. PF's got a method that can be used with a regular pot, check www.fanaticus.com if you don't have a pc'er.


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Servator]
    #889205 - 09/17/02 07:13 PM (19 years, 15 days ago)



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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: matts]
    #889209 - 09/17/02 07:15 PM (19 years, 15 days ago)

Yea, but I flame this kid so damn much. I feel like I owe him a freebie....
:grin:


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Servator]
    #889216 - 09/17/02 07:18 PM (19 years, 15 days ago)



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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 16 years, 5 months
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889217 - 09/17/02 07:18 PM (19 years, 15 days ago)

"if it gets contamunated, isnt it due to inocculating where you have to stick the syringe in the 4 holes on the top? "

The way that you have worded this makes it very hard to see what the question is. Contamination can be, and most of the time is introduced through innoculation, either due to a contaminated syringe, or filthy working conditions. Or it can be caused by improper sterilization.

About the 45-60 min in the PC, I've read some people only PC for like 20 min. (do a search to be sure.)


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Re-Defeat Bush in '04


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Skikid16]
    #889219 - 09/17/02 07:19 PM (19 years, 15 days ago)



Edited by matts (09/17/02 07:20 PM)


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OfflineXiC_clone
Calls Shots 'Round Here
 User Gallery

Registered: 08/27/02
Posts: 505
Loc: Earth 25XX
Last seen: 4 months, 25 days
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889282 - 09/17/02 07:42 PM (19 years, 15 days ago)

not..........................going......................to..................flame......

::runs away::


-XiC


--------------------
Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!


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Offlinecroftsk
cupcake

Registered: 07/19/02
Posts: 278
Last seen: 16 years, 6 months
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889522 - 09/17/02 09:34 PM (19 years, 15 days ago)

ya know, USAlaser would have been a much cooler name! :tongue: 


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Expose yourself to your deepest fear; after that, fear has no power, and the fear of freedom shrinks and vanishes.
You are free. - Jim Morrison


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OfflineShaw
hick

Registered: 06/28/01
Posts: 2,220
Loc: midwest, usa
Last seen: 9 months, 14 days
Re: dont flame me, sterilization questions [Re: XiC_clone]
    #889524 - 09/17/02 09:34 PM (19 years, 15 days ago)

yes you are you fucking liar


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InvisibleTM
The Mind, The Many, The Music.
Male User Gallery

Folding@home Statistics
Registered: 06/11/02
Posts: 8,282
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Re: dont flame me, sterilization questions [Re: croftsk]
    #889539 - 09/17/02 09:39 PM (19 years, 15 days ago)

I think he should've chosen "IMALoser" LOL! :grin: 


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================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineXiC_clone
Calls Shots 'Round Here
 User Gallery

Registered: 08/27/02
Posts: 505
Loc: Earth 25XX
Last seen: 4 months, 25 days
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889560 - 09/17/02 09:47 PM (19 years, 15 days ago)

If it is just the time that bothers ya, 20 mins @ 15 PSI will do the job for :
PF tek cakes
Wheat Berries
PF wheat berry cakes
Inverto's

I caught my first jar of contam about 3 weeks ago. I am guessing it was the shorter PC'ing time. But waiting for it to cool after 20 min's is a big difference than an hour. So I like the 20 min style.

-XiC


--------------------
Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 18 years, 10 months
Re: dont flame me, sterilization questions [Re: TM]
    #889569 - 09/17/02 09:52 PM (19 years, 15 days ago)

:laugh: heh! good idea but too late for that. maybe USEDbyLSA? how about LSAbuser?
or perhaps LSA2LSD

ok seriously do people ever break jars to get the mycellum cake out? someone said to break em. wont you get tiny pieces of glass, damage the cake and make a mess? also can you break the cake into pieces getting it out of the jar and bury it under soil in the fruiting container and let the shrooms grow out from the soil?
about the 4 holes, can 3 or even just 2 holes be used to reduce contamination?


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OfflineShaw
hick

Registered: 06/28/01
Posts: 2,220
Loc: midwest, usa
Last seen: 9 months, 14 days
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889577 - 09/17/02 09:57 PM (19 years, 15 days ago)

do you like getting flamed? whoever said that is an idiot.


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889581 - 09/17/02 09:58 PM (19 years, 15 days ago)

Ya gotta do what you gotta do...
Leave it at 4 for air exchange, makes it easier to innoculate more evenly as well. I don't think there would be any advantage to fewer holes.


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 18 years, 10 months
Re: dont flame me, sterilization questions [Re: Servator]
    #889594 - 09/17/02 10:04 PM (19 years, 15 days ago)

if air exchange was desired why block them using aluminum foil?


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889599 - 09/17/02 10:08 PM (19 years, 15 days ago)

You don't, jar lids or foil with holes punched only one of two.


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 18 years, 10 months
Re: dont flame me, sterilization questions [Re: Servator]
    #889619 - 09/17/02 10:23 PM (19 years, 15 days ago)

I see. I heard some mentioning of using more than one layer of foil, the first layer has 4 holes you inocculate then put 2 more layers of foil over that? even with 1/2 inch dry verm on top, contams can easily go thru after a week or so?


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889730 - 09/17/02 11:11 PM (19 years, 15 days ago)

Bat crap is useless as a substrate & so is this thread  :smirk:

Gonna flame, not gonna, gonna................ :mad: :grin: :mad: :grin: :mad: :grin: :ooo:

What's the point?

6T :tongue: 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineTRIPPYCHIK
PUPPET SLAVE
Registered: 03/15/02
Posts: 96
Loc: PUPPETVILLE
Last seen: 13 years, 2 months
Re: dont flame me, sterilization questions [Re: LSAuser]
    #890240 - 09/18/02 05:58 AM (19 years, 15 days ago)

I got away with just boiling the jars. Then I got smart and quickly realized that I wasn't gonna do cakes forever and invested in a All American 915 PC. That machine is serious ya heard. If you ever have any thoughts of advancing to grains later on, get a PC. Whether a Presto or All American just get it somehow.
Sell your body, sell crack, whatever it takes. Just kidding! or maybe not!

just my 2 sense!


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"SO FUCKIN WHAT IF IM A PUPPET SLAVE"


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Offlineblackout
 User Gallery

Registered: 07/17/00
Posts: 5,112
Last seen: 17 hours, 43 minutes
Trusted Cultivator
Re: dont flame me, sterilization questions [Re: TRIPPYCHIK]
    #890363 - 09/18/02 07:15 AM (19 years, 15 days ago)

366 posts and he still hasnt bothered to find the FAQs


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Offlinemyco_holic
mycologicalexplorer
Registered: 08/20/02
Posts: 138
Loc: SoCal
Last seen: 18 years, 6 months
Re: dont flame me, sterilization questions [Re: LSAuser]
    #890488 - 09/18/02 08:30 AM (19 years, 15 days ago)

Hey LSA ...I thought just like you did....On the 5th of Sept. I had boiled my jars for only 40 minutes. Not only that , but i think water got in them.... After they cooled i looked at them and thought , "Well they look clean , smell clean ." They must be clean then right? WRONG !!! After a strong gorwth the first day everything stopped .....Six days into the stop i noticed an odor similar to my gym socks after running a couple of miles. I popped the top and threw them in a plastic bag to stop the odor..... It really stinks..Moral of the story, If you want a smelly pile of shit pc for 10 min...You'll have some red funk growin in no time....Thats what you' re tryin to grow right?


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I believe in making the world safe for our children, but not our children's children, because I don't think children should be having sex.

Jack Handey.


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Offlinebladerunnerss
journeyman
Registered: 07/16/02
Posts: 64
Last seen: 7 years, 7 months
Re: dont flame me, sterilization questions [Re: myco_holic]
    #891756 - 09/18/02 07:40 PM (19 years, 14 days ago)

lmfao @/w/ myco haulic! =O


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bladeruNNer



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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: dont flame me, sterilization questions [Re: LSAuser]
    #891779 - 09/18/02 07:46 PM (19 years, 14 days ago)

"PENETRATION' it is all about penetration !
Are you sure you were the fastest sperm in the bunch ? :smirk: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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